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Degradation and detoxification of phenanthrene by actinobacterium Zhihengliuella sp. ISTPL4

  • Arti MishraEmail author
  • Rashmi Rathour
  • Rashmi Singh
  • Taruna Kumari
  • Indu Shekhar ThakurEmail author
Sustainable Industrial and Environmental Bioprocesses
  • 55 Downloads

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are universal environmental contaminants of great concern with regard to their potential exposure and deleterious effect on human health. The current study is the first report of phenanthrene degradation by a psychrotolerant (15 °C), halophilic (5% NaCl), and alkalophilic (pH 8) bacterial strain Zhihengliuella sp. ISTPL4, isolated from the sediment sample of the Pangong Lake, Ladakh, Jammu and Kashmir, India. Degradation studies revealed that the optimum specific growth rate was observed at 250 ppm of phenanthrene with 81% and 87% removal of phenanthrene in 72 h and 168 h, respectively. During the degradation of phenanthrene; 9,10-dihydrophenanthrene; 1-phenanthrenecarboxylic acid; and phthalic acid were detected as intermediates. Whole-genome sequencing of strain ISTPL4 has predicted phenanthrene; 9,10-monooxygense; and epoxide hydrolase B that are involved in the phenanthrene metabolism. Phenanthrene cytotoxicity was evaluated with human hepatic carcinoma cell line (HepG2) and it was observed that the cytotoxicity decreased with increased duration of bacterial incubation and maximum cell viability was observed at 168 h (89.92%). Our results suggest, Zhihengliuella sp. ISTPL4 may promise a great potential for environmental remediation applications.

Keywords

Polycyclic aromatic hydrocarbons Phenanthrene Biodegradation Zhihengliuella sp. ISTPL4 MTT assay Whole genome sequencing 

Notes

Acknowledgements

The authors are thankful to Dr. Kristina Medhi (SES, JNU, New Delhi) for helping in editing the manuscript. The authors are also thankful to AIRF and JNU for analytical analysis.

Funding

The authors would like to express their sincere thanks to the University Grant Commission (UGC), Government of India, UPoE-II, for financial support and also for providing the Dr. D.S. Kothari Fellowship to Arti Mishra and Rashmi Singh. Rashmi Rathour is grateful to the UGC: NFSC, New Delhi, for the Junior Research Fellowship.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

11356_2019_5478_MOESM1_ESM.docx (346 kb)
ESM 1 (DOCX 346 kb)

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© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of Environmental SciencesJawaharlal Nehru UniversityNew DelhiIndia
  2. 2.Department of StatisticsUniversity of DelhiNew DelhiIndia

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