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Environmental Science and Pollution Research

, Volume 26, Issue 19, pp 19560–19574 | Cite as

Oral administration of graphene oxide nano-sheets induces oxidative stress, genotoxicity, and behavioral teratogenicity in Drosophila melanogaster

  • Subhashree Priyadarsini
  • Shraban Kumar Sahoo
  • Swetapadma Sahu
  • Sumit Mukherjee
  • Garudadhwaj Hota
  • Monalisa MishraEmail author
Research Article

Abstract

The current study checks the effect of various concentrations of dietary graphene oxide (GO) nano-sheets on the development of Drosophila melanogaster. GO was synthesized and characterized by XRD, FTIR, FESEM, and TEM analytical techniques. Various concentrations of GO were mixed with the fly food and flies were transferred to the vial. Various behavioral and morphological as well as genetic defects were checked on the different developmental stages of the offspring. In the larval stage of development, the crawling speed and trailing path change significantly than the control. GO induces the generation of oxygen radicals within the larval hemolymph as evidenced by nitroblue tetrazolium assay. GO induces DNA damage within the gut cell, which was detected by Hoechst staining and within hemolymph by comet assay. Adult flies hatched after GO treatment show defective phototaxis and geotaxis behavior. Besides behavior, phenotypic defects were observed in the wing, eye, thorax bristles, and mouth parts. At 300 mg/L concentration, wing spots were observed. Altogether, the current study finds oral administration of GO which acts as a mutagen and causes various behavioral and developmental defects in the offspring. Here for the first time, we are reporting GO, which acts as a teratogen in Drosophila, besides its extensive medical applications.

Keywords

Nano-toxicity Bristle defect Wing spot assay Climbing assay Light/dark assay Comet assay 

Notes

Acknowledgments

Three anonymous reviewers are thankfully acknowledged for their valuable comments on the manuscript.

Funding information

SP and SM received financial support from MHRD. SS received financial support from DST-Inspire. GH and MM received financial support from DBT (Grant No. BT/PR21857/NNT/28/1238/2017).

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Subhashree Priyadarsini
    • 1
  • Shraban Kumar Sahoo
    • 2
  • Swetapadma Sahu
    • 1
  • Sumit Mukherjee
    • 1
  • Garudadhwaj Hota
    • 2
  • Monalisa Mishra
    • 1
    Email author
  1. 1.Neural Developmental Biology Lab, Department of Life ScienceNIT RourkelaRourkelaIndia
  2. 2.Department of ChemistryNIT RourkelaRourkelaIndia

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