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Environmental Science and Pollution Research

, Volume 26, Issue 19, pp 19468–19480 | Cite as

Phytochemical profile and insecticidal activity of Agave americana leaf extract towards Sitophilus oryzae (L.) (Coleoptera: Curculionidae)

  • Asma Mami MaazounEmail author
  • Soumaya Haouel Hamdi
  • Feten Belhadj
  • Jouda Mediouni Ben Jemâa
  • Chokri Messaoud
  • Mohamed Nejib Marzouki
Research Article
  • 150 Downloads

Abstract

The main objective of the present study is to introduce a new and ecologically safe method for managing the rice weevil, Sitophilus oryzae. Therefore, the Agave americana leaf extract’s phytochemical profile, and its insecticidal activity against the adults of S. oryzae were evaluated. The A. americana leaf extract was screened for the following phytochemicals: total phenolics (14.70 ± 0.31 mg GAE/g FW), total flavonoids (5.15 ± 0.18 mg RE/g FW) and saponins (10.32 ± 0.20 mg OAE/g FW). The HPLC-ESI/TOF-MS analysis results revealed that flavonoid glycosides (kaempferol, quercetin, and isorhamnetin derivates) were the major phenolic compounds of the A. americana leaf extract. In addition, the GC-MS analysis identified n-alkanes (77.77%) as significant compounds of the lipophilic fraction from the leaf extract. Moreover, the insecticidal potential was assessed through contact and repellent bioassays towards the rice weevil adults. The LD50, LC50, and RC50 values were 10.55 μg/insect, 8.99 μg/cm2, and 0.055 μg/cm2 for topical application method, treated filter-paper method, and repellent bioassay, respectively. Furthermore, the A. americana leaf extract inhibited digestive enzyme activities, and median inhibition concentrations IC50 were evaluated to be 146.06 ± 1.74 and 86.18 ± 1.08 μg/mL for α-amylase and protease, respectively. Overall, our results highlighted the promising potential of the leaf extract against S. oryzae adults, allowing us to recommend the extract under investigation as an ecofriendly alternative to synthetic insecticides.

Keywords

Botanical insecticide Phenolic compounds Saponins Rice weevil Contact toxicity Repellency 

Notes

Acknowledgments

This work was supported by the Ministry of Higher Education and Scientific Research, Tunisia.

Compliance with ethical standards

The authors reported no potential conflict of interests.This paper does not contain any experiments with human participants or any other vertebrates.

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Authors and Affiliations

  1. 1.Laboratory of Biotechnology Applied to Agriculture (LBAA), National Agricultural Research Institute of Tunisia, INRAT, Ministry of Higher Education and Scientific ResearchUniversity of CarthageTunis CedexTunisia
  2. 2.Laboratory of Protein Engineering and Bioactive Molecules, (LIP-MB), National Institute of Applied Sciences and Technology, INSAT, Ministry of Higher Education and Scientific ResearchUniversity of CarthageTunis CedexTunisia
  3. 3.Laboratory of Plant Biotechnology, National Institute of Applied Sciences and Technology, INSAT, Ministry of Higher Education and Scientific ResearchUniversity of CarthageTunis CedexTunisia

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