Environmental Science and Pollution Research

, Volume 26, Issue 18, pp 18240–18246 | Cite as

Effect of prenatal PFOS exposure on liver cell function in neonatal mice

  • Xiaoliu Liang
  • Guojie Xie
  • Xinmou Wu
  • Min SuEmail author
  • Bin YangEmail author
Research Article


Perfluorooctane sulfonate (PFOS), a hepatotoxic pollutant, is detected in the human cord blood, and it may induce health risk to an embryo. In this study, we established intrauterine exposure to PFOS in mice to evaluate potential impacts of PFOS on postnatal day 1 (PND1) offspring through conducting biochemical tests, quantitative PCR, and immunostaining. As results, PFOS-exposed maternal mice showed marked hepatomegaly and induced liver steatosis in a high dose of 5 mg PFOS/kg. In PND1 mice, intrahepatic contents of triglyceride, total cholesterol, and LDL were elevated by high-dose PFOS exposure, while intracellular HDL content was decreased. As shown in quantitative PCR, functional messenger RNAs of cytochrome P4A14 (CYP4A14) for fatty acid oxidation, CD36 for hepatic fatty acid uptake, and apolipoprotein B100 (APOB) and fibroblast growth factor 21 (FGF21) for hepatic export of lipids in PND1 livers were changed when compared to those in PFOS-free controls. In further validations, immunofluorescence stains showed that hepatic CYP4A14 and CD36 immunoreactive cells were increased in PFOS-exposed PND1 mice. In addition, reduced immunofluorescence-positive cells of APOB and FGF21 were observed in PND1 livers. Collectively, these preliminary findings demonstrate that prenatal exposure to PFOS may affect lipid metabolism in liver cells of PND1 mice.


Perfluorooctane sulfonate Prenatal exposure Lipid Liver cells 



Our study is granted in part by the National Natural Science Foundation of China (No. 81660091) and the National Natural Science Foundation of Guangxi (Nos. 2016GXNSFBA380055 and 2018GXNSFAA281242).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of PharmacyGuangxi Medical UniversityNanningChina
  2. 2.Department of Gynecology, Guigang City People’s HospitalThe Eighth Affiliated Hospital of Guangxi Medical UniversityGuigangPeople’s Republic of China
  3. 3.Key Laboratory of Tumor Immunology and Microenvironmental RegulationGuilin Medical UniversityGuilinPeople’s Republic of China

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