Environmental Science and Pollution Research

, Volume 26, Issue 18, pp 18403–18410 | Cite as

The effect of silver nanoparticles on the mutagenic and the genotoxic properties of the urban wastewater liquid sludges

  • Messaouda Khallef
  • Djamel Eddine Benouareth
  • Muhsin KonukEmail author
  • Recep Liman
  • Sara Bouchelaghem
  • Sara Hazzem
  • Khadra Kerdouci
Research Article


Nanoparticles are very effective compounds to transform and detoxicate common environmental contaminants. For this reason, crude urban liquid wastewater sludges were treated by silver nanoparticles (Ag-NPs, 100 nm) for 24 h. Both Ag-NPs’ treated and untreated sludges were examined for the evaluation if there are possible mutagenic/anti-mutagenic, cytotoxic, and genotoxic/anti-genotoxic effects by Ames and Allium cepa tests. The results were then subjected to statistical analyses by using SPSS software and p < 0.05 was accepted as a significant value. The data obtained from the Ames test showed that while untreated crude liquid sludge had a significant mutagenic effect, Ag-NP-treated one decreased its mutagenicity. Similar effects were also observed in the chromosome aberration-Allium cepa tests. Significant chromosome aberrations observed were C-metaphase, sticky metaphase, sticky anaphase, anaphase bridge, vagrant chromosome, and multipolar anaphases. Both tests demonstrated that silver nanoparticle treatment decreased the major mutagenicity and genotoxicity detected in the liquid wastewater sludges.


Wastewater sludges Ag-NPs Ames test Allium cepa test 



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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Faculté des Sciences de la Nature et de la Vie et des Sciences de la Terre et de l’UniversUniversité 8 Mai 1945 GuelmaGuelmaAlgeria
  2. 2.Department of Molecular Biology and Genetics, Faculty of Engineering and Natural SciencesÜsküdar UniversityIstanbulTurkey
  3. 3.Department of Molecular Biology and Genetics, Faculty of Arts and SciencesUsak UniversityUsakTurkey

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