Environmental Science and Pollution Research

, Volume 26, Issue 19, pp 19411–19424 | Cite as

Revealing the correlation of biomethane generation, DOM fluorescence, and microbial community in the mesophilic co-digestion of chicken manure and sheep manure at different mixture ratio

  • Liuying Song
  • Dunjie Li
  • Hongli Fang
  • Xiangyunong Cao
  • Rutao Liu
  • Qigui NiuEmail author
  • Yu-You Li
Research Article


Batch co-digestion tests of chicken manure (CM) and sheep manure (SM) at different ratio (Rs/c) were conducted under mesophilic condition (35 °C). Batch kinetic analysis of bioCH4 production, excitation-emission matrix (EEM) fluorescence of dissolved organic matter (DOM), and microbial community were investigated. The well-fitted modified Gompertz model (R2, 0.98–0.99) resulted that the co-digestion markedly improved the methane production rate and shortened the lag phase time. The highest bioCH4 yield of 219.67 mL/gVSadd and maximum production rate of 0.378 mL/gVSadd/h were obtained at an optimum Rs/c of 0.4. Additionally, a significant variation of DOM was detected at the Rs/c of 0.4 with a consistent degradation of soluble microbial byproduct-like and protein-like organics. The positive synergy effects of co-digestion conspicuously enhanced the bioCH4 production efficiency. FI370 and NADH were significantly correlated to Rs/c (p < 0.05). Moreover, the correlations among process indicator, EEM-peaks and different environmental parameters were evaluated by Pearson correlation analysis. The high diversity of acetoclastic methanogens and hydrogenotrophic methanogens in the co-digestion improved the stability of process.

Graphical Abstract


Chicken manure Sheep manure Co-digestion Rs/c EEM Microbial community 



Chicken manure


Sheep manure


Volatile solid ratio of sheep manure to chicken manure


Dissolved chemical oxygen demand


Total chemical oxygen demand


Dissolved organic matter


Three-dimensional excitation-emission matrix






Total solid


Volatile solid


Soluble microbial byproduct-like materials


The biological index


The humification index


Nicotinamide adenine dinucleotide (the reduced form)


Nicotinamide adenine dinucleotide (the oxidation form)


Total ammonia nitrogen


Free ammonia


Fluorescence regional integration


Total phosphorus


The seed sludge


The co-digestion sludge


The sludge setting in starvation


Anaerobic sludge


Funding information

The authors’ research is supported by the National Natural Science Foundation of China (Grant No. 51608304 and Grant No. U1806216) and Young Scholars Program of Shandong University (2018WLJH53). Research Fund of Tianjin Key Laboratory of Aquatic Science and Technology (TJKLAST-ZD-2017-04) and Research Fund of Jiangsu Key Laboratory of Anaerobic Biotechnology (JKLAB201702) were also partly supported this work. “The Fundamental Research Funds of Shandong University” and China Postdoctoral Science Foundation (2017M622209). also supported this study.

Supplementary material

11356_2019_5175_MOESM1_ESM.docx (28 kb)
ESM 1 (DOCX 28 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of Environmental Science and Engineering, China–America CRC for Environment & Health of Shandong ProvinceShandong UniversityQingdaoPeople’s Republic of China
  2. 2.Jiangsu Key Laboratory of Anaerobic BiotechnologyJiangnan UniversityWuxiPeople’s Republic of China
  3. 3.Department of Civil and Environmental Engineering, Graduate School of EngineeringTohoku UniversitySendaiJapan

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