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Environmental Science and Pollution Research

, Volume 26, Issue 18, pp 18440–18450 | Cite as

Nanoencapsulated methyl salicylate as a biorational alternative of synthetic antifungal and aflatoxin B1 suppressive agents

  • Anupam Kujur
  • Amrita Yadav
  • Akshay Kumar
  • Prem Pratap Singh
  • Bhanu PrakashEmail author
Research Article
  • 67 Downloads

Abstract

In view of the suspected negative impact of synthetic fungicides to the human health, nutritional quality, and non-targeted organisms, the use of plant-based antifungal agents has gained considerable interest to the agri-food industries. The aim of this study was to explore the antifungal and aflatoxin B1 (AFB1) inhibitory activity of chitosan (low molecular weight) encapsulated methyl salicylate. The nanoencapsulation of methyl salicylate (Ne-MS) has been characterized by SEM, FTIR, and XRD analysis. The encapsulation efficiency and loading capacity of Ne-MS ranged between 32–34% and 5–7% respectively. The minimum inhibitory concentration of Ne-MS (1.00 μL/mL) against the growth and aflatoxin B1 production by Aspergillus flavus was found to be lower than the free MS (1.50 μL/mL). Mode of action studies demonstrated that the Ne-MS cause a significant decrease in the ergosterol content, leakage of vital ions (Ca2+, Mg2+, and K+), utilization of different carbon source by the A. flavus. Further, the docking result showed ver1 and omt A gene of AFB1 biosynthesis are the possible molecular site of action of methyl salicylate. The in situ study revealed that Ne-MS had no significant negative impact on the organoleptic properties of the food system (maize) which strengthen its potential as a biorational alternative of synthetic fungicides.

Keywords

Antifungal Aflatoxin B1 Methyl salicylate Mode of action Nanoencapsulation 

Notes

Acknowledgements

We are thankful to the Head, CAS in Botany, Banaras Hindu University, Varanasi, for instrumental facilities. We are also thankful to Indian Institute of Technology, Banaras Hindu University, Varanasi, for the SEM and XRD analyses.

Funding information

Financial support was by CSIR (JRF-Ref: 09/013(0706)/2017-EMR-I) and Science and Engineering Research Board (Scheme No. ECR/2016/000299) New Delhi, India.

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Anupam Kujur
    • 1
  • Amrita Yadav
    • 1
  • Akshay Kumar
    • 1
  • Prem Pratap Singh
    • 1
  • Bhanu Prakash
    • 1
    Email author
  1. 1.Centre of Advanced Study in Botany, Institute of ScienceBanaras Hindu UniversityVaranasiIndia

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