Ameliorative effect of Bacillus subtilis, Saccharomyces boulardii, oregano, and calcium montmorillonite on growth, intestinal histology, and blood metabolites on Salmonella-infected broiler chicken

  • Alaeldein M. AbudabosEmail author
  • Hemiar A.A. Alhouri
  • Ibrahim A. Alhidary
  • Mohammed A. Nassan
  • Ayman A. Swelum
Research Article


This study evaluated the effects of Bacillus subtilis, Saccharomyces boulardii, oregano, and calcium montmorillonite on the physical growth, intestinal histomorphology, and blood metabolites in Salmonella-challenged birds during the finisher phase. In this study, a total of 600 chicks (Ross 308) were randomly distributed into the following dietary treatments: basal diet with no treatment; infected with Salmonella; T1, infected + avilamycin; T2, infected + Bacillus subtilis; T3, Saccharomyces boulardii; T4, infected + oregano; T5, infected + calcium montmorillonite. Our results indicated that feed consumption, body weight gain, total body weight, and feed conversion ratio increased significantly (P < 0.01) in T1 and T2. Villus width increased significantly (P < 0.01) in T1 while the total area was significantly (P < 0.01) higher in T1 and T2 among the treatment groups. Blood protein was significantly (P < 0.01) high in T3 and T4; however, the glucose concentration was significantly (P < 0.01) high in T2, T3, and T4. The treatments increased significantly (P< 0.01) in the treatment groups compared to the negative control. Aspirate aminotransferase (AST) was significantly (P < 0.05) low in T3 compared to the positive control. In conclusion, the results indicated that supplementation of Bacillus subtilis and calcium montmorillonite improved the production performance compared to other feed additives in broiler chicks infected with Salmonella during the finisher phase.


Chickens probiotic growth intestine biochemical 


Funding information

This study was financially supported by the Deanship of Scientific Research at King Saud University through Research Group Project No. RGP-273.

Compliance with ethical standards

The study was approved by the departmental committee on ethics and welfare of animals approved by the Department of Animal Production, King Saud University, Saudi Arabia.


  1. Abudabos AM, Alyemni AH, Dafalla YM, Khan RU (2016) The effect of phytogenic feed additives to substitute in-feed antibiotics on growth traits and blood biochemical parameters in broiler chicks challenged with Salmonella typhimurium. Environ Sci Pollut Res 23:24151–24157CrossRefGoogle Scholar
  2. Abudabos AM, Alyemni AH, Dafalla YM, Khan RU (2017) Effect of organic acid blend and Bacillus subtilis alone or in combination on growth traits, blood biochemical and antioxidant status in broilers exposed to Salmonella typhimurium challenge during the starter phase. J Appl Anim Res 45:538–542CrossRefGoogle Scholar
  3. Abudabos AM, Alyemni AH, Dafalla YM, Khan RU (2018) The effect of phytogenics on growth traits, blood biochemical and intestinal histology in broiler chickens exposed to Clostridium perfringens challenge. J Appl Anim Res 46:691–695CrossRefGoogle Scholar
  4. Alhidary IA, Abdelrahman MM, Uallh Khan R, Harron RM (2016) Antioxidant status and immune responses of growing camels supplemented a long acting multi trace minerals rumen bolus. Ital J Anim Sci 15:343–349CrossRefGoogle Scholar
  5. Bajpai VK, Baek KH, Kang SC (2012) Control of Salmonella in foods by using essential oils, A review. Food Res Intern 45:722–734CrossRefGoogle Scholar
  6. Chand N, Faheem H, Khan RU, Qureshi MS, Alhidary IA, Abudabos AM (2016) Anticoccidial effect of mananoligosacharide against experimentally induced coccidiosis in broiler. Environ Sci Pollut Res 23:14414–14421CrossRefGoogle Scholar
  7. Ching C, Cravens RL (2016) Calcium montmorillonite, based dietary supplement attenuates necrotic enteritis induced by Eimeria maxima and Clostridium perfringens in broilers. J Poult Sci 53:329–340CrossRefGoogle Scholar
  8. Khan RU, Naz S (2013) Application of probiotics in poultry production. World’s Poultry Sci J 69:621–632CrossRefGoogle Scholar
  9. Khan RU, Naz S, Nikousefat Z, Tufarelli V, Laudadio V (2012a) Thymus vulgaris; alternative to antibiotics in poultry feed. Worlds Poult Sci 68:401–408CrossRefGoogle Scholar
  10. Khan RU, Naz S, Nikousefat Z, Tufarelli V, Javdani M, Qureshi MS, Laudadio V (2012b) Potential applications of ginger (Zingiber officinale) in poultry diet. Worlds Poult Sci 68(2):245–252CrossRefGoogle Scholar
  11. Khan RU, Naz S, Javdani M, Nikousefat Z, Selvaggi M, Tufarelli V, Laudadio V (2012c) The use of turmeric (Curcuma longa) in poultry diets. World’s Poultry Sci J 68(1):97–103CrossRefGoogle Scholar
  12. Khan RU, Nikousefat Z, Tufarelli V, Naz S, Javdani M, Laudadio V (2012d) Garlic (Allium sativa) supplementation in poultry diet, effect on production and physiology. World’s Poultry Sci J 68:417–424CrossRefGoogle Scholar
  13. Khan RU, Naz S, Dhama K, Kathrik K, Tiwari R, Abdelrahman MM, Alhidary IA, Zahoor A (2016) Direct-fed microbial, beneficial applications, modes of action and prospects as a safe tool for enhancing ruminant production and safeguarding health. Int J Pharmacol 12:220–231CrossRefGoogle Scholar
  14. Lillehoj HS, Lee SH, Park SS, Jeong M, Lim Y, Mathis GF, Lumpkins B, Chi F, Ching C, Cravens RL (2016) Calcium montmorillonite-based dietary supplement attenuates necrotic enteritis induced by Eimeria maxima and Clostridium perfringens in broilers. J Poult Sci 53:329–340CrossRefGoogle Scholar
  15. National Research Council (1994) Nutrient requirements of poultry, 9th edn. National Academy of Sciences, Washington, DCGoogle Scholar
  16. Rahman S, Khan S, Chand N, Sadique U, Khan RU (2017) In vivo effects of Allium cepa L. on the selected gut microflora and intestinal histomorphology in broiler. Acta Histochem 119:446–450CrossRefGoogle Scholar
  17. SAS (2003) Statistical Analysis System user’s guide; Statistics version 9. SAS Institute, Cary, p 1Google Scholar
  18. Shahid SN, Chand RU, Khan SM, Suhail and NA Khan (2015). Alterations in cholesterol and fatty acids composition in egg yolk of Rhode Island Red x Fyoumi Hens Fed with Hemp Seeds (Cannabis sativa L.). Journal of Chemistry, Article ID 362936.
  19. Tehseen M, Tahir M, Khan RU, Jabbar A, Ahmad B, Ahsan T, Khan MS, Khan S, Abudabos AM (2016) Additive effect of Nigella sativa and Zingiber officinale herbal mixture on performance and cholesterol profile in broiler. Philipp Agric Sci 99:408–413Google Scholar
  20. Zia Rahman, S Naz, Khan RU, and Tahir M (2017). An update on the potential application of L-carnitine in poultry. World’s Poultry Science Journal 73: 823–830Google Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Alaeldein M. Abudabos
    • 1
    Email author
  • Hemiar A.A. Alhouri
    • 1
  • Ibrahim A. Alhidary
    • 1
  • Mohammed A. Nassan
    • 2
  • Ayman A. Swelum
    • 1
  1. 1.Department of Animal Production, College of Food and Agriculture SciencesKing Saud UniversityRiyadhSaudi Arabia
  2. 2.Department of Pathology, Faculty of Veterinary MedicineZagazig UniversityZagazigEgypt

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