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Environmental Science and Pollution Research

, Volume 26, Issue 18, pp 18509–18519 | Cite as

Efficient biodegradation of polyethylene (HDPE) waste by the plastic-eating lesser waxworm (Achroia grisella)

  • Harsha Kundungal
  • Manjari Gangarapu
  • Saran Sarangapani
  • Arunkumar Patchaiyappan
  • Suja Purushothaman DevipriyaEmail author
Research Article
  • 167 Downloads

Abstract

Polyethylene (PE) is one of the major persistent plastic that is not biodegradable at considerable rates in most environments, and is the major source of unceasing environmental pollution. Recently, biodegradation of plastic wastes through waxworms and mealworms were reported. The present study focuses on the high-density polyethylene (HDPE) degradation capabilities of the larvae of Achroia grisella (lesser waxworm) and its ability to complete its life cycle when fed with HDPE. Effects of added nutrition on PE degradation were assessed, providing wax comb as co-feed (PE-WC). The egested frass of the waxworm fed on waxcomb (WC), PE, and PE-WC were studied by analyzing the changes in physiochemical properties through FTIR and 1H NMR techniques in addition to weight loss percentage of PE and survival rates of the tested lesser waxworms. The post-degradation studies of WC and PE showed 90.5 ± 1.2% and 43.3 ± 1.6% weight loss, respectively, by a group of 100 lesser waxworms. Over an 8-day period, PE consumption increased with an ingestion of 1.83 mg of PE per day per larvae. Supplementing the PE feed of lesser waxworms with WC facilitated enhanced PE degradation showing 69.6 ± 3.2% weight loss. Twenty-eight day survival rates for lesser waxworms fed on WC, PE, and PE-WC were 91.3 ± 1.01%, 74.6 ± 2.9%, and 86 ± 1.4%, respectively. The FTIR and 1H NMR analysis of egested frass indicated formation of new functional organic groups, supporting biodegradation of PE in lesser waxworms. The frass of the lesser waxworm fed on PE samples shows the presence of new carbonyl and alcoholic groups with increase in unsaturated hydrocarbon indicating formation of biodegraded intermediates. Lesser waxworms fed with WC, PE, and PE-WC completed all life cycle stages (larvae, pupae, moth, and egg) developing into a second generation. The second generation of PE-WC fed larvae of A. grisella efficiently degrades PE at par with first generation counterparts.

Keywords

Plastic wastes Polyethylene Biodegradation Waxworm Achroia grisella 

Notes

Acknowledgements

The authors are thankful to the Central Instrumentation Facility, Pondicherry University, India, for providing the instrumentation facility for characterization. The financial support from Maulana Azad National Fellowship program, University Grants Commission, India (F1-17.1/2015-16/MANF-2015-17-KER-65616) is acknowledged.

Supplementary material

11356_2019_5038_MOESM1_ESM.docx (2.5 mb)
ESM 1 (DOCX 2555 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Harsha Kundungal
    • 1
  • Manjari Gangarapu
    • 1
  • Saran Sarangapani
    • 1
  • Arunkumar Patchaiyappan
    • 1
  • Suja Purushothaman Devipriya
    • 1
    Email author
  1. 1.Department of Ecology and Environmental SciencesPondicherry UniversityPuducherryIndia

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