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Environmental Science and Pollution Research

, Volume 26, Issue 11, pp 10790–10799 | Cite as

Bacterial indicator reduction in dairy manure using hybrid zero-valent iron (h-ZVI) system

  • Sunghwa Han
  • Yongheng Huang
  • Zong LiuEmail author
Research Article

Abstract

Novel and efficient animal wastewater treatment technologies of bacteria reduction are needed for preventing disease outbreak in animal herds and safeguarding environmental health. Zero-valent iron (ZVI) has been used to treat bacteria contaminated water for the past decades, but its passivation issue has been a major challenge. In this study, batch tests were performed to evaluate the effect of a hybrid zero-valent iron (h-ZVI) or a mixed ZVI/Fe3O4 media system on reduction of Escherichia coli (E. coli) levels. The h-ZVI media was created through a wet chemical process that uses nitrate to oxidize ZVI in the presence of externally added Fe2+ (aq.). Transforming ZVI into a h-ZVI system could overcome the passivation of ZVI and increase the reactivity of the media. The results demonstrated that E. coli cells in the bulk phase were removed rapidly by h-ZVI media. Majority of E. coli was attached (or adsorbed) to the surface of h-ZVI media within a few minutes, which suggested that adsorption was the dominant mechanism for bacterial removal in the initial phase. This adsorption was confirmed by fluorescence microscopy with CTC-DAPI double staining and transmission electron microscopy (TEM). Increasing contact time steadily inactivated E. coli; all cells were inactivated after 120 min of contact. The TEM results indicated that h-ZVI inactivated E. coli by causing direct damage on bacterial cell membrane. The results of this study strongly suggest that h-ZVI treatment can be used in water treatment industry where bacterial contamination is concerned.

Keywords

Hybrid zero-valent iron (h-ZVI) Pathogen indicator reduction Manure water treatment Bacterial adsorption Bacterial inactivation Bacterial removal mechanism 

Abbreviations

CFU

Colony forming unit

CTC

Tetrazolium salt 5-cyano-2,3-ditolyltetrazolium chloride

DAPI

4′,6-Diamidino-2-phenylindole

DDI water

Deoxygenated and deionized water

E. coli

Escherichia coli

h-ZVI

Hybrid zero-valent iron

LB

Luria-Bertani

OD

Optical density

Rpm

Revolutions per minute

TEM

Transmission electron microscopy

TSB/TSA

Tryptic soy broth/Agar

UV

Ultraviolet

Notes

Acknowledgements

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors, but was supported by Texas A&M Agrilife and administered by the Department of Bio and Agricultural engineering at Texas A&M University.

Supplementary material

11356_2019_4501_MOESM1_ESM.docx (2 mb)
ESM 1 (DOCX 2069 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Biological and Agricultural EngineeringTexas A&M UniversityCollege StationUSA

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