Environmental Science and Pollution Research

, Volume 26, Issue 10, pp 10148–10158 | Cite as

Ionomic profile and arsenic speciation in Semisulcospira cancellata, a freshwater shellfish from a mine-impacted river in China

  • Fan Du
  • Lin WangEmail author
  • Zhaoguang Yang
  • Peng Liu
  • Deliang LiEmail author
Research Article


Freshwater aquatic environment close to cities and industrial areas is more sensitive than marine environment. The freshwater shellfish Semisulcospira cancellate was introduced as a bioindicator to monitor the heavy metal contamination in the river through ionomic profiles and arsenic speciation. The shellfish samples were collected near four cities along the Xiang River in China. The concentrations of elements including Mg, Al, Ca, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, As, Sr, Cd, Sn, Sb, Ba, and Pb were determined using ICP-MS. Multivariate statistical analyses such as Pearson’s correlation analysis and principle component analysis (PCA) were employed to identify the possible sources of the elements in the shellfish samples. Three principle components were extracted from the ionomic matrix and were associated with natural existence, biological pathways, and mining and smelting activities, respectively. The ionomic profiles of the shellfish samples were evaluated through hierarchical cluster analysis (HCA) which was exhibited in the form of heatmap. The shellfish samples were categorized according to the sampling sites with different contamination levels. Six As species including arsenite [As(III)], arsenate [As(V)], monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), arsenobetaine (AsB), and arsenocholine (AsC) were separated and quantified using HPLC-ICP-MS. The concentrations of As(III) and As(V) were linearly increased with total As concentration increasing. However, the proportion of AsB was decreased with total As while the AsB concentration was irrelevant to total As.


Bioindicator Ionomics Heavy metal Heatmap Arsenobetaine Biotransformation 


Funding information

This work was financially supported by Natural Science Foundation of China (Grant No. 21607175) and Special Fund for Agro-scientific Research in the Public Interest of China (No. 201503108).

Supplementary material

11356_2019_4489_MOESM1_ESM.pdf (599 kb)
ESM 1 (PDF 598 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Chemistry and Chemical EngineeringCentral South UniversityChangshaChina
  2. 2.Center for Environment and Water ResourcesCentral South UniversityChangshaChina
  3. 3.Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, College of Animal Science and TechnologyHunan Agricultural UniversityChangshaChina

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