Environmental Science and Pollution Research

, Volume 26, Issue 10, pp 9508–9523 | Cite as

Green sol–gel synthesis of novel nanoporous copper aluminosilicate for the eradication of pathogenic microbes in drinking water and wastewater treatment

  • Bahaa Ahmed HemdanEmail author
  • Amany Mohamed El Nahrawy
  • Abdel-Fatah M. Mansour
  • Ali Belal Abou Hammad
Research Article


We used a green sol–gel synthesis method to fabricate a novel nanoporous copper aluminosilicate (CAS) material. Nanoporous CAS was characterized using X-ray powder diffraction (XRD), field emission transmission and scanning electron microscopies (FE-TEM/FE-SEM), Fourier transform infrared (FTIR) spectroscopy, and optical analyses. The CAS was also evaluated for use as a promising disinfectant for the inactivation of waterborne pathogens. The antimicrobial action and minimum inhibitory concentration (MIC) of this CAS disinfectant were determined against eight microorganisms (Escherichia coli, Salmonella enterica, Pseudomonas aeruginosa, Listeria monocytogenes, Staphylococcus aureus, Enterococcus faecalis, Candida albicans, and Aspergillus niger). An antimicrobial susceptibility testing of CAS was measured. Results of disc diffusion method pointed out that the diameters of the zone using well diffusion were wider than disc diffusion methods, and the findings also showed that the MIC of the CAS disinfectant against E. coli, S. enterica, and P. aeruginosa was 100 mg/L within 20 min of contact time. Meanwhile, the MIC of the CAS disinfectant was 100 mg/L within 40 min of contact time for the other strains. The efficacy of antimicrobial action (100%) reached within 20 to 40 min against all tested microbes. Herein, the antimicrobial susceptibility testing of CAS disinfectant showed no toxicity for human and bacterial cells. It can be concluded that nanoporous CAS is a promising, economically, and worthy weapon for water disinfection.


Green sol–gel Nanoporous CAS Pathogenic microbes Disinfection Water purification 


Funding information

The authors would like to thank the National Research Centre (NRC), Egypt, for their financial support and providing the equipment required.

Compliance with ethical standards

Conflict of interests

The authors declare that they have no conflict of interest.


  1. Alghamdi H, Dakhane A, Alum A, Abbaszadegan M, Mobasher B, Neithalath N (2018) Synthesis and characterization of economical, multi-functional porous ceramics based on abundant aluminosilicates. Mater Des 152:10–21. CrossRefGoogle Scholar
  2. American Public Health Association, American Water Works Association, Water Environment Federation (2012) Standard methods for the examination of water and wastewater, 22nd edn. Am Public Heal Assoc, Washingto, DC, USA ISBN 9780875532356Google Scholar
  3. Bagchi B, Kar S, Dey SK, Bhandary S, Roy D, Mukhopadhyay TK, Das S, Nandy P (2013) In situ synthesis and antibacterial activity of copper nanoparticle loaded natural montmorillonite clay based on contact inhibition and ion release. Colloids Surf B Biointerfaces. 108:358–365. CrossRefGoogle Scholar
  4. Bian N, Mayanovic RA, Benamara M (2018) Synthesis and characterization of Co3O4-MnxCo3-xO4Core-Shell nanoparticles. MRS Advances, InGoogle Scholar
  5. Bogdanović U, Lazić V, Vodnik V, Budimir M, Marković Z, Dimitrijević S (2014) Copper nanoparticles with high antimicrobial activity. Mater Lett 128:75–78. CrossRefGoogle Scholar
  6. Chaturvedi KS, Henderson JP (2014) Pathogenic adaptations to host-derived antibacterial copper. Front Cell Infect Microbiol 4.
  7. Craun MF, Craun GF, Calderon RL, Beach MJ (2006) Waterborne outbreaks reported in the United States. J Water Health 4:19–30. CrossRefGoogle Scholar
  8. Dankovich TA, Smith JA (2014) Incorporation of copper nanoparticles into paper for point-of-use water purification. Water Res 63:245–251. CrossRefGoogle Scholar
  9. De Velasco-Maldonado PS, Hernández-Montoya V, Montes-Morán MA et al (2018) Surface modification of a natural zeolite by treatment with cold oxygen plasma: characterization and application in water treatment. Appl Surf Sci 434:1193–1199. CrossRefGoogle Scholar
  10. Dollwet HHA, Sorenson JRJ (1985) Historic uses of copper compounds in medicine. Trace Elem Med 2:80–87Google Scholar
  11. El Hotaby W, Sherif HHA, Hemdan BA et al (2017) Assessment of in situ-prepared polyvinylpyrrolidone-silver nanocomposite for antimicrobial applications. Acta Phys Pol A 131:1554–1560. CrossRefGoogle Scholar
  12. Elith J, Leathwick JR (2009) Species distribution models: ecological explanation and prediction across space and time. Annu Rev Ecol Evol Syst 40:677–697. CrossRefGoogle Scholar
  13. El Nahrawy AM (2015) Structural studies of sol gel prepared nano-crystalline silica zinc titanate ceramic. Intr J Advanc Engin Technol Computer Sci 2:15–18Google Scholar
  14. El Nahrawy A, AbouHammad AB (2016) A facile co-gelation sol gel route to synthesize cao: P2o5: Sio2 xerogel embedded in chitosan nanocomposite for bioapplications. Int J Pharm Tech Res 9:16–21Google Scholar
  15. El Nahrawy AM, Ali AI (2014) Influence of reaction conditions on sol-gel process producing SiO2 and SiO2 -P2O5 gel and glass. J Glas Ceram 04:42–47.
  16. El Nahrawy AM, Kim YS, Ali AI (2016) Synthesis of hybrid chitosan/calcium aluminosilicate using a sol-gel method for optical applications. J Alloys Compd 676:432–439.
  17. El Nahrawy AM, Moez AA, Saad AM (2018) Sol-gel preparation and spectroscopic properties of modified sodium silicate /Tartrazine dye nanocomposite. Silicon 10:2117–2122.
  18. Elsaesser A, Howard CV (2012) Toxicology of nanoparticles. Adv Drug Deliv Rev 64:129–137CrossRefGoogle Scholar
  19. Elwakeel KZ, El-Liethy MA, Ahmed MS et al (2018) Facile synthesis of magnetic disinfectant immobilized with silver ions for water pathogenic microorganism’s deactivation. Environ Sci Pollut Res 25:22797–22809. CrossRefGoogle Scholar
  20. Esteban-Cubillo A, Pecharromán C, Aguilar E, et al (2006) Antibacterial activity of copper monodispersed nanoparticles into sepiolite. In: J Materials Sci 41(16):5208–5212.
  21. Farag AAM, Mansour AM, Ammar AH, Rafea MA, Farid AM (2012) Electrical conductivity, dielectric properties and optical absorption of organic based nanocrystalline sodium copper chlorophyllin for photodiode application. J Alloys Compd 513:404–413. CrossRefGoogle Scholar
  22. Fenwick A (2006) Waterborne infectious diseases - could they be consigned to history?. Science 313(5790):1077–1081.
  23. Flokstra BR, Van Aken B, Schnoor JL (2008) Microtox® toxicity test: detoxification of TNT and RDX contaminated solutions by poplar tissue cultures. Chemosphere 71:1970–1976. CrossRefGoogle Scholar
  24. Ford TE (1999) Microbiological safety of drinking water: United States and global perspectives. Environ Health Perspect 107:191. CrossRefGoogle Scholar
  25. Gaballah ST, El-Nazer HA, Abdel-Monem RA et al (2019) Synthesis of novel chitosan-PVC conjugates encompassing Ag nanoparticles as antibacterial polymers for biomedical applications. Int J Biol Macromol 121:707–717. CrossRefGoogle Scholar
  26. Grass G, Rensing C, Solioz M (2011) Metallic copper as an antimicrobial surface. Appl Environ Microbiol 77:1541–1547CrossRefGoogle Scholar
  27. Guo X, Li W, Nakanishi K, Kanamori K, Zhu Y, Yang H (2013) Preparation of mullite monoliths with well-defined macropores and mesostructured skeletons via the sol-gel process accompanied by phase separation. J Eur Ceram Soc 33:1967–1974. CrossRefGoogle Scholar
  28. Gupta N, Pant P, Gupta C, Goel P, Jain A, Anand S, Pundir A (2018) Engineered magnetic nanoparticles as efficient sorbents for wastewater treatment: a review. Mater Res Innov 22:434–450. CrossRefGoogle Scholar
  29. Hao OJ, Lin C-F, Fu-Tien J, Chien-Jen S (1995) A review of Microtox test and its applications. Toxicol Environ Chem 52:57–76CrossRefGoogle Scholar
  30. Hellard ME, Sinclair MI, Forbes AB, Fairley CK (2001) A randomized, blinded, controlled trial investigating the gastrointestinal health effects of drinking water quality. Environ Health Perspect 109:773–778. CrossRefGoogle Scholar
  31. Hisatomi T, Kubota J, Domen K (2014) Recent advances in semiconductors for photocatalytic and photoelectrochemical water splitting. Chem Soc Rev 43:7520–7535CrossRefGoogle Scholar
  32. Hongquan Z, Yuhua Y, Youfa W, Shipu L (2003) Morphology and formation mechanism of hydroxyapatite whiskers from moderately acid solution. Mater Res 6:111–115CrossRefGoogle Scholar
  33. Jamil TS, Mansor ES, Azab El-Liethy M (2015) Photocatalytic inactivation of E. Coli using nano-size bismuth oxyiodide photocatalysts under visible light. J Environ Chem Eng 3:2463–2471. CrossRefGoogle Scholar
  34. Jin R, Yang Y, Xing Y, Chen L, Song S, Jin R (2014) Facile synthesis and properties of hierarchical double-walled copper silicate hollow nanofibers assembled by nanotubes. ACS Nano 8:3664–3670. CrossRefGoogle Scholar
  35. Jin Y, Deng J, Liang J, Shan C, Tong M (2015) Efficient bacteria capture and inactivation by cetyltrimethylammonium bromide modified magnetic nanoparticles. Colloids Surf B Biointerfaces 136:659–665. CrossRefGoogle Scholar
  36. Jones GL (1973) Bacterial growth kinetics: measurement and significance in the activated-sludge process. Water Res 7:1475–1492. CrossRefGoogle Scholar
  37. Kajihara K (2013) Recent advances in sol-gel synthesis of monolithic silica and silica-based glasses. J Asian Ceram Soc 1(2):121–133.
  38. Karpanen TJ, Casey AL, Lambert PA, Cookson BD, Nightingale P, Miruszenko L, Elliott TSJ (2012) The antimicrobial efficacy of copper alloy furnishing in the clinical environment: a crossover study. Infect Control Hosp Epidemiol 33:3–9. CrossRefGoogle Scholar
  39. Kaur K, Singh KJ, Anand V, Bhatia G, Singh S, Kaur H, Arora DS (2016) Magnesium and silver doped CaO–Na2O–SiO2–P2O5 bioceramic nanoparticles as implant materials. Ceram Int 42:12651–12662. CrossRefGoogle Scholar
  40. Kaur P, Singh KJ, Yadav AK, Sood H, Kaur S, Kaur R, Arora DS, Kaur S (2018) Preliminary investigation of the effect of doping of copper oxide in CaO-SiO2-P2O5-MgO bioactive composition for bone repair applications. Mater Sci Eng C 83:177–186. CrossRefGoogle Scholar
  41. Khezerlou A, Alizadeh-Sani M, Azizi-Lalabadi M, Ehsani A (2018) Nanoparticles and their antimicrobial properties against pathogens including bacteria, fungi, parasites and viruses. Microb Pathog 123:505–526. CrossRefGoogle Scholar
  42. Kim YH, Lee DK, Cha HG, Kim CW, Kang YC, Kang YS (2006a) Preparation and characterization of the antibacterial Cu nanoparticle formed on the surface of SiO2 nanoparticles. J Phys Chem B 110:24923–24928. CrossRefGoogle Scholar
  43. Kim YH, Lee DK, Cha HG, Kim CW, Kang YC, Kang YS (2006b) Preparation and characterization of the antibacterial Cu nanoparticle formed on the surface of SiO2 nanoparticles. J Phys Chem B 110:24923–24928. CrossRefGoogle Scholar
  44. Kim YH, Lee DK, Cha HG, Kim CW, Kang YS (2007) Synthesis and characterization of antibacterial Ag - SiO2nanocomposite. J Phys Chem C 111:3629–3635. CrossRefGoogle Scholar
  45. Köck R, Becker K, Cookson B, et al (2010) Methicillin-resistant staphylococcus aureus (MRSA): burden of disease and control challenges in Europe. Eurosurveillance 15(41):19688.
  46. Kunz AN, Brook I (2010) Emerging resistant gram-negative aerobic bacilli in hospital-acquired infections. Chemotherapy 56:492–500. CrossRefGoogle Scholar
  47. Mahapatra O, Bhagat M, Gopalakrishnan C, Arunachalam KD (2008) Ultrafine dispersed CuO nanoparticles and their antibacterial activity. J Exp Nanosci 3:185–193. CrossRefGoogle Scholar
  48. Maniprasad P, Santra S (2012) Novel copper (cu) loaded core-shell silica nanoparticles with improved cu bioavailability: synthesis, characterization and study of antibacterial properties. J Biomed Nanotechnol 8:558–566. CrossRefGoogle Scholar
  49. Mansour AM, El-Menyawy EM, Mahmoud GM et al (2017a) Structural, optical and galvanomagnetic properties of nanocrystalline se 51.43 in 44.67 Pb 3.9 thin films. Mater Res Express 4:115903. CrossRefGoogle Scholar
  50. Mansour AM, El-Taweel FMAA, Abu El-Enein RANN, El-Menyawy EM (2017b) Structural, optical, electrical and photoelectrical properties of 2-Amino-4-(5-bromothiophen-2-yl)-5,6-dihydro-6-methyl-5-oxo-4H-pyrano[3,2-c] quinoline-3-carbonitrile films. J Electron Mater 46:1–8. CrossRefGoogle Scholar
  51. Navarro E, Baun A, Behra R, Hartmann NB, Filser J, Miao AJ, Quigg A, Santschi PH, Sigg L (2008) Environmental behavior and ecotoxicity of engineered nanoparticles to algae, plants, and fungi. Ecotoxicology 17:372–386CrossRefGoogle Scholar
  52. Øye G, Glomm WR, Vrålstad T, Volden S, Magnusson H, Stöcker M, Sjöblom J (2006) Synthesis, functionalisation and characterisation of mesoporous materials and sol-gel glasses for applications in catalysis, adsorption and photonics. Adv Colloid Interf Sci 123-126:17–32CrossRefGoogle Scholar
  53. PA W (2015) CLSI Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard—tenth edition. CLSI document M07-A10. Clinical and Laboratory Standards Institute, Wayne, PAGoogle Scholar
  54. Pandey PK, Kass PH, Soupir ML, et al (2014) Contamination of water resources by pathogenic bacteria. AMB Express 4:51.
  55. Raffi M, Mehrwan S, Bhatti TM, Akhter JI, Hameed A, Yawar W, ul Hasan MM (2010) Investigations into the antibacterial behavior of copper nanoparticles against Escherichia coli. Ann Microbiol 60:75–80. CrossRefGoogle Scholar
  56. Ren G, Hu D, Cheng EWC, Vargas-Reus MA, Reip P, Allaker RP (2009a) Characterisation of copper oxide nanoparticles for antimicrobial applications. Int J Antimicrob Agents 33:587–590. CrossRefGoogle Scholar
  57. Ren G, Hu D, Cheng EWC, Vargas-Reus MA, Reip P, Allaker RP (2009b) Characterisation of copper oxide nanoparticles for antimicrobial applications. Int J Antimicrob Agents 33:587–590. CrossRefGoogle Scholar
  58. Reyes-Jara A, Cordero N, Aguirre J, Troncoso M, Figueroa G (2016) Antibacterial effect of copper on microorganisms isolated from bovine mastitis. Front Microbiol 7:1–10. CrossRefGoogle Scholar
  59. Reynolds KA, Mena KD, Gerba CP (2008) Risk of waterborne illness via drinking water in the United States. Rev Environ Contam Toxicol 192:117–158.
  60. Rice LB (2007) Emerging issues in the management of infections caused by multidrug-resistant gram-negative bacteria. Cleve Clin J Med 74:S12. CrossRefGoogle Scholar
  61. Sedlak DL, Von Gunten U (2011) The chlorine dilemma. Science 331(6013):42–43.
  62. Sharma RK, Pant P (2009) Preconcentration and determination of trace metal ions from aqueous samples by newly developed gallic acid modified Amberlite XAD-16 chelating resin. J Hazard Mater 163:295–301. CrossRefGoogle Scholar
  63. Simões M, Simões LC, Vieira MJ (2010) A review of current and emergent biofilm control strategies. LWT - Food Sci Technol 43:573–583CrossRefGoogle Scholar
  64. Singh A, Krishna V, Angerhofer A, Do B, MacDonald G, Moudgil B (2010) Copper coated silica nanoparticles for odor removal. Langmuir 26:15837–15844. CrossRefGoogle Scholar
  65. Tiwari DK, Behari J, Sen P (2008) Application of nanoparticles in waste water treatment. Carbon Nanotub 3:417–433. CrossRefGoogle Scholar
  66. Tripathi VS, Kandimalla VB, Ju H (2006) Preparation of ormosil and its applications in the immobilizing biomolecules. Sensors Actuators B Chem 114:1071–1082Google Scholar
  67. Trojanowicz M, Bojanowska-Czajka A, Bartosiewicz I, Kulisa K (2018) Advanced oxidation/reduction processes treatment for aqueous perfluorooctanoate (PFOA) and perfluorooctanesulfonate (PFOS) – a review of recent advances. Chem Eng J 336:170–199CrossRefGoogle Scholar
  68. UNCF (2014) The State of the World’s Children 2014 -Every Child Counts.United Nations Children’s Fund 2014.Google Scholar
  69. United States Environmental Protection Agency (2017) Clean Water Act Section 303(d): Impaired Waters and Total Maximum Daily Loads (TMDLs). In: United States Environ. Prot. AgencyGoogle Scholar
  70. Usman MS, Zowalaty ME, El Shameli K et al (2013) Synthesis, characterization, and antimicrobial properties of copper nanoparticles. Int J Nanomedicine 8:4467–4479Google Scholar
  71. Vincent M, Hartemann P, Engels-Deutsch M (2016) Antimicrobial applications of copper. Int J Hyg Environ Health 219:585–591. CrossRefGoogle Scholar
  72. Wang Y, Lin F, Shang B, Peng B, Deng Z (2018) Self-template synthesis of nickel silicate and nickel silicate/nickel composite nanotubes and their applications in wastewater treatment. J Colloid Interface Sci 522:191–199. CrossRefGoogle Scholar
  73. WHO (2012) Good health adds life to years - global brief for world health day 2012. World Heal Organ.
  74. WHO (2014) GLASS 2014 Report. Investing in water and sanitation: increasing access, reducing inequalities - UN-water global analysis and assessment of sanitation and drinking water. World Health Organization, GenevaGoogle Scholar
  75. Yoon KY, Hoon Byeon J, Park JH, Hwang J (2007) Susceptibility constants of Escherichia coli and Bacillus subtilis to silver and copper nanoparticles. Sci Total Environ 373:572–575. CrossRefGoogle Scholar
  76. Young M, Santra S (2014) Copper (Cu)-silica nanocomposite containing valence-engineered Cu: a new strategy for improving the antimicrobial efficacy of cu biocides. J Agric Food Chem 62:6043–6052. CrossRefGoogle Scholar
  77. Youssef AM, El-Nahrawy AM, Abou Hammad AB (2017) Sol-gel synthesis and characterizations of hybrid chitosan-PEG/calcium silicate nanocomposite modified with ZnO-NPs and (E102) for optical and antibacterial applications. Int J Biol Macromol 97:561–567. CrossRefGoogle Scholar
  78. Zazouli MA, Kalankesh LR (2017) Removal of precursors and disinfection byproducts (DBPs) by membrane filtration from water; a review. J Environ Heal Sci Eng 15(1):25.
  79. Zhan S, Yang Y, Shen Z, Shan J, Li Y, Yang S, Zhu D (2014) Efficient removal of pathogenic bacteria and viruses by multifunctional amine-modified magnetic nanoparticles. J Hazard Mater 274:115–123. CrossRefGoogle Scholar
  80. Zhan G, Yec CC, Zeng HC (2015) Mesoporous bubble-like manganese silicate as a versatile platform for design and synthesis of nanostructured catalysts. Chem - A Eur J 21:1882–1887. CrossRefGoogle Scholar
  81. Zhang S, Fu R, Dingcai W et al (2004) Preparation and characterization of antibacterial silver-dispersed activated carbon aerogels. Carbon N Y 42:3209–3216. CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Water Pollution Research Department, Environmental Research DivisionNational Research CentreGizaEgypt
  2. 2.Solid-State Physics Department, Physics Research DivisionNational Research CentreGizaEgypt

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