Environmental Science and Pollution Research

, Volume 26, Issue 10, pp 9931–9937 | Cite as

Algae turf scrubber and vertical constructed wetlands combined system for decentralized secondary wastewater treatment

  • Gleison de Souza CelenteEmail author
  • Gustavo Stolzenberg Colares
  • Ênio Leandro Machado
  • Eduardo Alexis Lobo
Research Article


Water shortage is a current problem faced by many regions. The deterioration of water bodies driven by the directly discard of untreated wastewater worsens the water shortage and implies in more costly treatments to meet local standards for water quality. In rural areas, the problem is even worse, once conventional centralized treatment plants do not encompass them. Decentralized treatment systems must present low-cost, local availability, standards-meeting efficiency, and simplified operation. The present study examines the combined use of algae turf scrubber and down-flow vertical constructed wetlands for a University’s sanitary wastewater treatment. After a hydraulic detention time of 21 days, the unit was able to reach 49%, 48%, 98%, 82%, 99.2%, 70.1%, 44%, 83%, 72%, 86%, 69%, 95%, and 99.9% for conductivity, total soluble solids, turbidity, apparent color, N-NH3, total nitrogen, P-soluble, total carbon, chemical oxygen demand, inorganic carbon, TOC, Escherichia coli, and total coliforms. In accord to the Brazilian standard ABNT 13969/97, the treated effluent is eligible for reuse in floor and sidewalks washing, garden irrigation, and landscaping purposes.


Decentralized treatment Algae turf scrubber Vertical constructed wetland Ecological engineering 


Funding information

This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001.


  1. Abdel-raouf N, Al-Homaidan A et al (2012) Microalgae and wastewater treatment. Saudi J Biol Sci 19(3):257–275 ISSN 1319-562XCrossRefGoogle Scholar
  2. ABNT, NBR 13969. (1997) Septic tanks - complementary treatment units and final disposal of liquid effluents - design, construction and operation, (In Portuguese)Google Scholar
  3. Andrade L, O'Dwyer J, O'Neill E, Hynds P (2018) Surface water flooding, groundwater contamination, and enteric disease in developed countries: a scoping review of connections and consequences. Environ Pollut 236:540–549CrossRefGoogle Scholar
  4. APHA (2012) Standard methods for the examination of water and wastewater. American Public Health AssociationGoogle Scholar
  5. Association Of Official Analytical Chemists, A. (2000) Official methods of analysis of the Association of Official Analytical Chemists. Association of Official Analytical Chemists., ISBN 0066-961XGoogle Scholar
  6. Ayaz SÇ, Aktaş Ö, Findik N, Akça L (2012) Phosphorus removal and effect of adsorbent type in a constructed wetland system. Desalin Water Treat 37(1–3):152–159 ISSN 1944-3994CrossRefGoogle Scholar
  7. Borges-Pedro JP et al (2018) Assessment of WASH scenarios in urban and rural schools of a small city in the Brazilian Amazon. Acta Amazon 48(1):75–82CrossRefGoogle Scholar
  8. Breen PF (1990) A mass balance method for assessing the potential of artificial wetlands for wastewater treatment. Water Res 24(6):689–697 ISSN 0043-1354CrossRefGoogle Scholar
  9. Coogan MA et al (2007) Algal bioaccumulation of triclocarban, triclosan, and methyl-triclosan in a North Texas wastewater treatment plant receiving stream. Chemosphere 67(10):1911–1918 5// 2007. ISSN 0045–6535. Available in: < // >CrossRefGoogle Scholar
  10. De-Bashan LE et al (2004) Microalgae growth-promoting bacteria as “helpers” for microalgae: a novel approach for removing ammonium and phosphorus from municipal wastewater. Water Res 38(2):466–474 1// 2004. ISSN 0043–1354. Available in: < // >CrossRefGoogle Scholar
  11. De Almeida M et al. (2015) Cation and anion monitoring in a wastewater treatment pilot project. Revista Facultad de Ingeniería Universidad de Antioquia, n. 76, p. 82–89. ISSN 0120-6230Google Scholar
  12. De Oliveira R et al. (1999) Relation between conductivity and total dissolved solids in samples from raw sewage and stabilization ponds. Brazilian Congress of Sanitary and Environmental Engineering 20° Feira Internacional de Tecnologias de Saneamento Ambiental, 3, ABES. p.1–6. (In Portuguese)Google Scholar
  13. Fonseca JCL, Silva MRA et al. (2006) Assessment of the analytical reliability of the total organic carbon (TOC) determinations. Eclet. Quím., São Paulo , v. 31, n. 3, p. 47–52. (In Portuguese)Google Scholar
  14. Gorazda K, Wzorek Z, Tarko B, Nowak AK, Kulczycka J, Henclik A (2013) Phosphorus cycle-possibilities for its rebuilding. Acta Biochim Pol 60(4):725–730 ISSN 0001-527XGoogle Scholar
  15. Gray NF (2004) Biology of wastewater treatment. World Scientific. ISBN 1783261188Google Scholar
  16. Gross A et al (2007) Recycled vertical flow constructed wetland (RVFCW)—a novel method of recycling greywater for irrigation in small communities and households. Chemosphere 66(5):916–923 1// 2007. ISSN 0045–6535. Available in: < >CrossRefGoogle Scholar
  17. Hai FI et al. (2014) Trace organic contaminants removal by combined processes for wastewater reuse. In: (Ed.). Advanced treatment technologies for urban wastewater reuse: Springer. p.39–77Google Scholar
  18. Horn TB, Zerwes FV, Kist LT, Machado ÊL (2014) Constructed wetland and photocatalytic ozonation for university sewage treatment. Ecol Eng 63:134–141 ISSN 0925-8574CrossRefGoogle Scholar
  19. Ji G et al (2002) Constructed subsurface flow wetland for treating heavy oil-produced water of the Liaohe Oilfield in China. Ecol Eng 18(4):459–465 2002/03/01/ 2002. ISSN 0925–8574. Available in: < >CrossRefGoogle Scholar
  20. Kadlec RH, Wallace S (2008) Treatment wetlands. CRC press. ISBN 1420012517Google Scholar
  21. Li Y et al (2011) Characterization of a microalga Chlorella sp. well adapted to highly concentrated municipal wastewater for nutrient removal and biodiesel production. Bioresour Technol 102(8):5138–5144 4// 2011. ISSN 0960–8524. Available in: < // >CrossRefGoogle Scholar
  22. Liao X, Nair VD, Canion A, Dobberfuhl DR, Foster DK, Inglett PW (2019) Subsurface transport and potential risk of phosphorus to groundwater across different land uses in a karst springs basin, Florida USA. Geoderma 338:97–106CrossRefGoogle Scholar
  23. Maberly S, Spence D (1983) Photosynthetic inorganic carbon use by freshwater plants. J Ecol, p. 705–724. ISSN 0022–0477Google Scholar
  24. Machado A et al. (2016) Overview of the state of the art of constructed wetlands for decentralized wastewater management in Brazil. J Environ Manag. ISSN 0301-4797Google Scholar
  25. Machado Ê et al. (2015) Constructed Wetlands Integrated with Advanced Oxidation Processes in Wastewater Treatment for Reuse. In: (Ed.). Advanced Treatment Technologies for Urban Wastewater Reuse: Springer. p.197-222Google Scholar
  26. Marker AFH, Nusch H et al (1980) The measurement of photosynthetic pigments in freshwater and standardization of methods: conclusion and recommendations. Arch Hydrobiol Beih 14:91–106Google Scholar
  27. Mcnabb DE (2017) Managing recycled water. In: water resource management. Palgrave Macmillan, Cham. p. 283–306Google Scholar
  28. Mehrabadi A, Craggs R, Farid MM (2015) Wastewater treatment high rate algal ponds (WWT HRAP) for low-cost biofuel production. Bioresour Technol 184:202–214CrossRefGoogle Scholar
  29. Mohr G, Lobo EA (2013) Evaluation of the efficiency of a small-scale water treatment system using bioassays. Revista Jovens Pesquisadores, v. 3, n. 1. ISSN 2237-048X. (In Portuguese)Google Scholar
  30. Mulbry W et al (2008) Treatment of dairy manure effluent using freshwater algae: algal productivity and recovery of manure nutrients using pilot-scale algal turf scrubbers. Bioresour Technol 99(17):8137–8142 11// 2008. ISSN 0960–8524. Available in: < // >CrossRefGoogle Scholar
  31. Nogueira R, Brito AG, Machado AP, Janknecht P, Salas JJ, Vera L, Martel G (2009) Economic and environmental assessment of small and decentralized wastewater treatment systems. Desalin Water Treat 4(1–3):16–21 ISSN 1944-3994CrossRefGoogle Scholar
  32. Oswald WJ et al (1953) Algae symbiosis in oxidation ponds: III. Photosynthetic oxygenation. Sewage and industrial wastes 25(6):692–705 ISSN 0096364X. Available in: < >Google Scholar
  33. Phillips P et al (2015) Concentrations of hormones, pharmaceuticals and other micropollutants in groundwater affected by septic systems in New England and New York. Sci Total Environ 512:43–54CrossRefGoogle Scholar
  34. Pinney ML, Westerhoff PK et al (2000) Transformations in dissolved organic carbon through constructed wetlands. Water Res 34(6):1897–1911 ISSN 0043-1354CrossRefGoogle Scholar
  35. Powley HR, Dürr HH, Lima AT, Krom MD, van Cappellen P (2016) Direct discharges of domestic wastewater are a major source of phosphorus and nitrogen to the Mediterranean Sea. Environ Sci Technol 50(16):8722–8730CrossRefGoogle Scholar
  36. Prochaska C, Zouboulis A (2006) Removal of phosphates by pilot vertical-flow constructed wetlands using a mixture of sand and dolomite as substrate. Ecol Eng 26(3):293–303 ISSN 0925-8574CrossRefGoogle Scholar
  37. Reardon DJ (1995) Turning down the power. Civ Eng 65(8):54 ISSN 0885-7024Google Scholar
  38. Renuka N et al (2013. ISSN 1573–5176. Available in: <>) Evaluation of microalgal consortia for treatment of primary treated sewage effluent and biomass production. J Appl Phycol 25(5):1529–1537.
  39. Rodriguez-Garcia G, Molinos-Senante M, Hospido A, Hernández-Sancho F, Moreira MT, Feijoo G (2011) Environmental and economic profile of six typologies of wastewater treatment plants. Water Res 45(18):5997–6010 ISSN 0043-1354CrossRefGoogle Scholar
  40. Ruiz-Marin A, Mendoza-Espinosa LG et al (2010) Growth and nutrient removal in free and immobilized green algae in batch and semi-continuous cultures treating real wastewater. Bioresour Technol 101(1):58–64 1// 2010. ISSN 0960–8524. Available in: < // >CrossRefGoogle Scholar
  41. Ruiz-Martinez A et al (2012) Microalgae cultivation in wastewater: nutrient removal from anaerobic membrane bioreactor effluent. Bioresour Technol 126:247–253 12// 2012. ISSN 0960–8524. Available in: < // >CrossRefGoogle Scholar
  42. Salati E Jr, Salati E et al (1999) Wetland projects developed in Brazil. Water Sci Technol 40(3):19–25 // 1999. ISSN 0273–1223. Available in: < // >CrossRefGoogle Scholar
  43. Scholz RW et al (2013) Sustainable use of phosphorus: a finite resource. Sci Total Environ 461–462:799–803 ISSN 0048–9697. Available in: < >CrossRefGoogle Scholar
  44. Silveira EO, Moura D, Rieger A, Machado ÊL, Lutterbeck CA (2017) Performance of an integrated system combining microalgae and vertical flow constructed wetlands for urban wastewater treatment. Environ Sci Pollut Res 24:20469–20478. CrossRefGoogle Scholar
  45. Shankar B (2011) Low-cost treatment for attenuation of nitrate from groundwater. J Eng Technol Res 3(1):16–21 ISSN 2006-9790Google Scholar
  46. Steinmann CR, Weinhart S, Melzer A (2003) A combined system of lagoon and constructed wetland for an effective wastewater treatment. Water Res 37(9):2035–2042 ISSN 0043-1354CrossRefGoogle Scholar
  47. Stottmeister U, Wießner A, Kuschk P, Kappelmeyer U, Kästner M, Bederski O, Müller RA, Moormann H (2003) Effects of plants and microorganisms in constructed wetlands for wastewater treatment. Biotechnol Adv 22(1):93–117 ISSN 0734-9750CrossRefGoogle Scholar
  48. Sturm BS, Lamer SL (2011) An energy evaluation of coupling nutrient removal from wastewater with algal biomass production. Appl Energy 88(10):3499–3506 ISSN 0306-2619CrossRefGoogle Scholar
  49. Sukačová K, Trtílek M, Rataj T (2015) Phosphorus removal using a microalgal biofilm in a new biofilm photobioreactor for tertiary wastewater treatment. Water Res 71:55–63 ISSN 0043-1354CrossRefGoogle Scholar
  50. Van Den Hende S et al (2016) Microalgal bacterial flocs originating from aquaculture wastewater treatment as diet ingredient for Litopenaeus vannamei (Boone). Aquac Res 47(4):1075–1089CrossRefGoogle Scholar
  51. Verhoeven JTA, Meuleman AFM (1999) Wetlands for wastewater treatment: opportunities and limitations. Ecol Eng 12(1):5–12 ISSN 0925–8574. Available in: < >CrossRefGoogle Scholar
  52. Vymazal J (2007) Removal of nutrients in various types of constructed wetlands. Sci Total Environ 380(1–3):48–65 ISSN 0048–9697. Available in: < >CrossRefGoogle Scholar
  53. Wang XH, Wang X, Huppes G, Heijungs R, Ren NQ (2015) Environmental implications of increasingly stringent sewage discharge standards in municipal wastewater treatment plants: case study of a cool area of China. J Clean Prod 94:278–283CrossRefGoogle Scholar
  54. WHO. Sanitation. 2015. Available in: < >. Acesso em: 14/06/2016
  55. WHO (2014). Progress on sanitation and drinking water: 2014 update. World Health OrganizationGoogle Scholar
  56. Wießner A et al (2005) Influence of the redox condition dynamics on the removal efficiency of a laboratory-scale constructed wetland. Water Res 39(1):248–256 ISSN 0043–1354. Available in: < >CrossRefGoogle Scholar
  57. Zhao YJ, Hui Z, Chao X, Nie E, Li HJ, He J, Zheng Z (2011) Efficiency of two-stage combinations of subsurface vertical down-flow and up-flow constructed wetland systems for treating variation in influent C/N ratios of domestic wastewater. Ecol Eng 37(10):1546–1554 ISSN 0925-8574CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Graduate Program in Environmental TechnologyUniversidade de Santa Cruz do Sul – UNISCSanta Cruz do SulBrazil

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