Environmental Science and Pollution Research

, Volume 26, Issue 9, pp 8928–8938 | Cite as

Beneficial influences of pelelith and dicyandiamide on gaseous emissions and the fungal community during sewage sludge composting

  • Jishao JiangEmail author
  • Youwei Pan
  • Xianli Yang
  • Juan Liu
  • Haohao Miao
  • Yuqing Ren
  • Chunyan Zhang
  • Guangxuan Yan
  • Jinghua Lv
  • Yunbei LiEmail author
Research Article


Reducing the emissions of NH3 and greenhouse gases (GHGs) during composting is essential for improving compost quality and controlling environmental pollution. This paper investigates the effects of pelelith (P) combined with dicyandiamide (DCD) on gaseous emissions and the fungal community diversity during sewage sludge (SS) composting. Results showed that the P and P + DCD treatments decreased the cumulative gaseous emissions by 41% and 22% for NH3, 21% and 34% for N2O, and 31.5% and 33.0% for CH4, respectively. The evolution of the fungal community analysis showed that Ascomycota and unclassified fungi dominated during the thermophilic stage, while only Ascomycota was the dominant fungal phylum during the maturity stage, composing 62%, 66%, and 73% of the total fungal community in the control, P, and P + DCD, respectively. The P and P + DCD significantly increased the fungal community richness at the genus level. Fungal community abundance was found to be significantly related to temperature, pH, organic matter, and total Kjeldahl nitrogen, which also influence the gaseous emissions during SS composting. It suggested that the combined addition of pelelith and dicyandiamide (DCD) was an effective method for reducing the emissions of NH3 and greenhouse gases during SS composting.


Composting Pelelith Dicyandiamide NH3 Greenhouse gases Fungal community 


Funding information

Financial support for this investigation was provided by National Natural Science Foundation of China (41805123, 51508167, and 41807327), the Key Research Project of Colleges and Universities for Education Department of Henan Province (17A610006 and 17B610006), and Natural Science Foundation of Henan Province of China (182300410153).


  1. Awasthi MK, Wang Q, Ren X, Zhao J, Huang H, Awasthi SK, Lahori AH, Li R, Zhou L, Zhang Z (2016) Role of biochar amendment in mitigation of nitrogen loss and greenhouse gas emission during sewage sludge composting. Bioresour Technol 219:270–280CrossRefGoogle Scholar
  2. Awasthi MK, Li J, Kumar S, Awasthi SK, Wang Q, Chen H, Wang M, Ren X, Zhang Z (2017) Effects of biochar amendment on bacterial and fungal diversity for co-composting of gelatin industry sludge mixed with organic fraction of municipal solid waste. Bioresour Technol 246:214–223CrossRefGoogle Scholar
  3. Awasthi MK, Wang Q, Awasthi SK, Wang M, Chen H, Ren X, Zhao J, Zhang Z (2018a) Influence of medical stone amendment on gaseous emissions, microbial biomass and abundance of ammonia oxidizing bacteria genes during biosolids composting. Bioresour Technol 247:970–979CrossRefGoogle Scholar
  4. Awasthi MK, Wang Q, Chen H, Wang M, Awasthi SK, Ren X, Cai H, Li R, Zhang Z (2018b) In-vessel co-composting of biosolid: focusing on mitigation of greenhouse gases emissions and nutrients conservation. Renew Energy 129:814–823CrossRefGoogle Scholar
  5. Barrington S, Choiniere D, Trigui M, Knight W (2002) Effect of carbon source on compost nitrogen and carbon losses. Bioresour Technol 83:189–194CrossRefGoogle Scholar
  6. Bernal MP, Alburquerque JA, Moral R (2009) Composting of animal manures and chemical criteria for compost maturity assessment. A review. Bioresour Technol 100:5444–5453CrossRefGoogle Scholar
  7. Boulter JI, Trevors JT, Boland GJ (2002) Microbial studies of compost: bacterial identification, and their potential for turfgrass pathogen suppression. World J Microbiol Biotechnol 18:661–671CrossRefGoogle Scholar
  8. Chan MT, Selvam A, Wong JW (2016) Reducing nitrogen loss and salinity during ‘struvite’ food waste composting by zeolite amendment. Bioresour Technol 200:838–844CrossRefGoogle Scholar
  9. Chowdhury MA, de Neergaard A, Jensen LS (2014) Potential of aeration flow rate and bio-char addition to reduce greenhouse gas and ammonia emissions during manure composting. Chemosphere 97:16–25CrossRefGoogle Scholar
  10. Deer WA (1992) An introduction to rock-forming minerals. Longman Scientific & Technical 66:509–517Google Scholar
  11. Edgar RC (2010) Search and clustering orders of magnitude faster than BLAST. Bioinformatics 26:2460–2461CrossRefGoogle Scholar
  12. Gomez-Brandon M, Lazcano C, Dominguez J (2008) The evaluation of stability and maturity during the composting of cattle manure. Chemosphere 70:436–444CrossRefGoogle Scholar
  13. Groat LE (1992) The chemistry of vesuvianite. Can Mineral 30:19–48Google Scholar
  14. Haddadin MS, Haddadin J, Arabiyat OI, Hattar B (2009) Biological conversion of olive pomace into compost by using Trichoderma harzianum and Phanerochaete chrysosporium. Bioresour Technol 100:4773–4782CrossRefGoogle Scholar
  15. IPCC (2007) Climate change 2007: impacts, adaptation and vulnerability. Contribution of working group II to the fourth assessment report of the intergovernmental panel on climate change. Cambridge University Press, CambridgeGoogle Scholar
  16. Jain MS, Jambhulkar R, Kalamdhad AS (2018) Biochar amendment for batch composting of nitrogen rich organic waste: effect on degradation kinetics, composting physics and nutritional properties. Bioresour Technol 253:204–213CrossRefGoogle Scholar
  17. Jeong YK, Hwang SJ (2005) Optimum doses of Mg and P salts for precipitating ammonia into struvite crystals in aerobic composting. Bioresour Technol 96:1–6CrossRefGoogle Scholar
  18. Jiang J, Huang Y, Liu X, Huang H (2014) The effects of apple pomace, bentonite and calcium superphosphate on swine manure aerobic composting. Waste Manag 34:1595–1602CrossRefGoogle Scholar
  19. Jiang T, Ma X, Tang Q, Yang J, Li G, Schuchardt F (2016) Combined use of nitrification inhibitor and struvite crystallization to reduce the NH3 and N2O emissions during composting. Bioresour Technol 217:210–218CrossRefGoogle Scholar
  20. Jiang J, Kang K, Chen D, Liu N (2017) Impacts of delayed addition of N-rich and acidic substrates on nitrogen loss and compost quality during pig manure composting. Waste Manag 72:161–167CrossRefGoogle Scholar
  21. Jiang J, Kang K, Wang C, Sun X, Dang S, Wang N, Wang Y, Zhang C, Yan G, Li Y (2018) Evaluation of total greenhouse gas emissions during sewage sludge composting by the different dicyandiamide added forms: mixing, surface broadcasting, and their combination. Waste Manag 81:94–103CrossRefGoogle Scholar
  22. Kelliher FM, Clough TJ, Clark H, Rys G, Sedcole JR (2008) The temperature dependence of dicyandiamide (DCD) degradation in soils: a data synthesis. Soil Biol Biochem 40:1878–1882CrossRefGoogle Scholar
  23. Khan E, Khaodhir S, Ruangrote D (2009) Effects of moisture content and initial pH in composting process on heavy metal removal characteristics of grass clipping compost used for stormwater filtration. Bioresour Technol 100:4454–4461CrossRefGoogle Scholar
  24. Khan N, Clark I, Sánchez-Monedero MA, Shea S, Meier S, Bolan N (2014) Maturity indices in co-composting of chicken manure and sawdust with biochar. Bioresour Technol 168:245–251CrossRefGoogle Scholar
  25. Kulikowska D (2016) Kinetics of organic matter removal and humification progress during sewage sludge composting. Waste Manag 49:196–203CrossRefGoogle Scholar
  26. Lam SK, Suter H, Mosier AR, Chen D (2016) Using nitrification inhibitors to mitigate agricultural N2O emission: a double-edged sword? Glob Chang Biol 23:485–489CrossRefGoogle Scholar
  27. Li H, Ru J, Yin W, Liu X, Wang J, Zhang W (2009) Removal of phosphate from polluted water by lanthanum doped vesuvianite. J Hazard Mater 168(1):326–330CrossRefGoogle Scholar
  28. Li YB, Jin PF, Liu TT, Lv JH, Jiang JS (2018) A novel method for sewage sludge composting using bamboo charcoal as a separating material. Environ Sci Pollut Res 1–12Google Scholar
  29. Liu N, Zhou J, Han L, Ma S, Sun X, Huang G (2017) Role and multi-scale characterization of bamboo biochar during poultry manure aerobic composting. Bioresour Technol 241:190–199CrossRefGoogle Scholar
  30. Luo Y, Li G, Luo W, Schuchardt F, Jiang T, Xu D (2013) Effect of phosphogypsum and dicyandiamide as additives on NH3, N2O and CH4 emissions during composting. J Environ Sci 25:1338–1345CrossRefGoogle Scholar
  31. Man TC, Selvam A, Wong JWC (2016) Reducing nitrogen loss and salinity of “struvite” food waste composting by zeolite amendment. Bioresour Technol 200:838–844CrossRefGoogle Scholar
  32. Mao H, Lv Z, Sun H, Li R, Zhai B, Wang Z, Awasthi MK, Wang Q, Zhou L (2018) Improvement of biochar and bacterial powder addition on gaseous emission and bacterial community in pig manure compost. Bioresour Technol 258:195–202CrossRefGoogle Scholar
  33. Meng L, Li W, Zhang S, Wu C, Lv L (2017) Feasibility of co-composting of sewage sludge, spent mushroom substrate and wheat straw. Bioresour Technol 226:39–45CrossRefGoogle Scholar
  34. Meng L, Zhang S, Gong H, Zhang X, Wu C, Li W (2018) Improving sewage sludge composting by addition of spent mushroom substrate and sucrose. Bioresour Technol 253:197–203CrossRefGoogle Scholar
  35. Metcalf JL, Xu ZZ, Weiss S, Lax S, Van TW, Hyde ER, Song SJ, Amir A, Larsen P, Sangwan N (2016) Microbial community assembly and metabolic function during mammalian corpse decomposition. Science 351:158–162CrossRefGoogle Scholar
  36. Neher DA, Weicht TR, Bates ST, Leff JW, Fierer N (2013) Changes in bacterial and fungal communities across compost recipes, preparation methods, and composting times. PLoS One 8:e79512CrossRefGoogle Scholar
  37. Nsereko VL, Beauchemin KA, Morgavi DP, Rode LM, Furtado AF, Mcallister TA, Iwaasa AD, Yang WZ, Wang Y (2002) Effect of a fibrolytic enzyme preparation from Trichoderma longibrachiatum on the rumen microbial population of dairy cows. Can J Microbiol 48:14–20CrossRefGoogle Scholar
  38. Pan Y, Tian S, Zhao Y, Zhang L, Zhu X, Gao J, Huang W, Zhou Y, Song Y, Zhang Q (2018) Identifying ammonia hotspots in China using a national observation network. Environ Sci Technol 52:3926–3934CrossRefGoogle Scholar
  39. Sanchez A, Artola A, Font X, Gea T, Barrena R, Gabriel D, Sanchez-Monedero MA, Roig A, Cayuela ML, Mondini C (2015) Greenhouse gas emissions from organic waste composting. Environ Chem Lett 13:223–238CrossRefGoogle Scholar
  40. Santos C, Goufo P, Fonseca J, Pereira JLS, Ferreira L, Coutinho J, Trindade H (2018) Effect of lignocellulosic and phenolic compounds on ammonia, nitric oxide and greenhouse gas emissions during composting. J Clean Prod 171:548–556CrossRefGoogle Scholar
  41. Sommer SG, Møller HB (2000) Emission of greenhouse gases during composting of deep litter from pig production-effect of straw content. J Agric Sci 134:327–335CrossRefGoogle Scholar
  42. Tian X, Yang T, He J, Chu Q, Jia X, Huang J (2017) Fungal community and cellulose-degrading genes in the composting process of Chinese medicinal herbal residues. Bioresour Technol 241:374–383CrossRefGoogle Scholar
  43. Tortosa G, Castellano-Hinojosa A, Correa-Galeote D, Bedmar EJ (2016) Evolution of bacterial diversity during two-phase olive mill waste (“alperujo”) composting by 16S rRNA gene pyrosequencing. Bioresour Technol 224:101–111CrossRefGoogle Scholar
  44. Wang Q, Wang Z, Awasthi MK, Jiang Y, Li R, Ren X, Zhao J, Shen F, Wang M, Zhang Z (2016) Evaluation of medical stone amendment for the reduction of nitrogen loss and bioavailability of heavy metals during pig manure composting. Bioresour Technol 220:297–304CrossRefGoogle Scholar
  45. Wang K, Yin X, Mao H, Chu C, Tian Y (2018a) Changes in structure and function of fungal community in cow manure composting. Bioresour Technol 255:123–130CrossRefGoogle Scholar
  46. Wang Q, Awasthi MK, Ren X, Zhao J, Li R, Wang Z, Wang M, Chen H, Zhang Z (2018b) Combining biochar, zeolite and wood vinegar for composting of pig manure: the effect on greenhouse gas emission and nitrogen conservation. Waste Manag 74:221–230CrossRefGoogle Scholar
  47. Wei H, Wang L, Hassan M, Xie B (2018) Succession of the functional microbial communities and the metabolic functions in maize straw composting process. Bioresour Technol 256:333–341CrossRefGoogle Scholar
  48. Yang F, Li G, Shi H, Wang Y (2015) Effects of phosphogypsum and superphosphate on compost maturity and gaseous emissions during kitchen waste composting. Waste Manag 36:70–76CrossRefGoogle Scholar
  49. Yu M, Zhang J, Xu Y, Xiao H, An W, Xi H, Xue Z, Huang H, Chen X, Shen A (2015) Fungal community dynamics and driving factors during agricultural waste composting. Environ Sci Pollut Res 22:19879–19886CrossRefGoogle Scholar
  50. Zhang J, Chen M, Sui Q, Tong J, Jiang C, Lu X, Zhang Y, Wei Y (2016a) Impacts of addition of natural zeolite or a nitrification inhibitor on antibiotic resistance genes during sludge composting. Water Res 91:339–349CrossRefGoogle Scholar
  51. Zhang J, Sui Q, Li K, Chen M, Tong J, Qi L, Wei Y (2016b) Influence of natural zeolite and nitrification inhibitor on organics degradation and nitrogen transformation during sludge composting. Environ Sci Pollut Res 23:1324–1334CrossRefGoogle Scholar
  52. Zhang L, Zhang H, Wang Z, Chen G, Wang L (2016c) Dynamic changes of the dominant functioning microbial community in the compost of a 90-m3 aerobic solid state fermentor revealed by integrated meta-omics. Bioresour Technol 203:1–10CrossRefGoogle Scholar
  53. Zhang B, Wang MM, Wang B, Xin Y, Gao J, Liu H (2018) The effects of bio-available copper on macrolide antibiotic resistance genes and mobile elements during tylosin fermentation dregs co-composting. Bioresour Technol 251:230–237CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Jishao Jiang
    • 1
    Email author
  • Youwei Pan
    • 1
  • Xianli Yang
    • 1
  • Juan Liu
    • 1
  • Haohao Miao
    • 1
  • Yuqing Ren
    • 1
  • Chunyan Zhang
    • 1
  • Guangxuan Yan
    • 1
  • Jinghua Lv
    • 1
  • Yunbei Li
    • 1
    Email author
  1. 1.School of Environment, Key Laboratory for Yellow River and Huai River Water Environmental and Pollution Control, Ministry of Education, Henan Key Laboratory for Environmental Pollution ControlHenan Normal UniversityXinxiangChina

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