Effects of exposure to triphenyltin (TPT) contaminant on sperm activity in adulthood of Calomys laucha exposed through breastfeeding
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Triphenyltin (TPT) is an organotin compound (OT), primarily used in agriculture and in the composition of antifouling paints for ships worldwide. Studies have showed its effects as an endocrine disrupter in several organisms by preventing enzymatic expression and causing reproductive toxicity. This study aimed to evaluate the effects of exposure to TPT, via breastfeeding, on reproductive physiology in the Calomys laucha species. The experimental design was compound of five groups, two controls and three with different doses of TPT. Moreover, females were exposed by gavage to the TPT for 20 days, from the 1st day postpartum to the 21st postnatal day (PND). Then, the pups were euthanized and the kinetics, organelles, and biochemistry of the sperm were evaluated. The results presented a reduction in total motility in the groups exposed to TPT. Regarding cellular organelles analysis, a loss in membrane integrity was evidenced; the functionality of mitochondria showed diminution followed by increased acrosome reaction. In conclusion, the TPT causes alteration of the reproductive parameters, decreasing the activity and sperm quality in individuals exposed in the breastfeeding phase.
KeywordsTriphenyltin TPT Breastfeeding Sperm activity Spermatic kinetics
Compliance with ethical standards
All procedures were carried out in accordance with applicable standards and in the normative resolutions and guidelines of the National Council for Control of Animal Experimentation, approved by the Ethics Committee on Animal Use (CEUA-FURG) under the number Pq017 / 2014.
- Abdollahi M, ranjbar A, shadnia S, nikfar S, rezaie A (2004) Pesticides and oxidative stress: a review. Med Sci Monit 10:141–147Google Scholar
- Colares EP (1997) Aspectos da Aspectos da fisiologia reprodutiva de Calomys laucha OLFERS, 1818 (Mammalia: Rodentia). Tese de doutorado, USP – São PauloGoogle Scholar
- De Simplicio P, Dacasto P, Carletti M, Giannerini F, Nebbia C (2000) Changes in hepatic and renal glutathione-dependent enzyme activities in rabbits and lambs subchronically treated with triphenyltin acetate. Vet Hum Toxicol 42(3):159–162Google Scholar
- Domínguez-Rebolledo AE, Martínez-Pastor F, Bisbal AF, Ros-Santaella JL, García-Álvarez O, Maroto-Morales A, Soler AJ, Garde JJ, Fernández-Santos MR (2011) Response of thawed epidydimal red deer spermatozoa to increasing concentrations of hydrogen peroxide, and importance of individual male variability. Reprod Domest Anim 46:93–403CrossRefGoogle Scholar
- FAO Pesticide residues in food (1991) Evaluations, part I – residues. Food and Agriculture Organization of the United Nations, Rome, pp 337–371Google Scholar
- Fitas AL, Amaral D, Lopes L (2014) Aromatase inhibitors in male adolescents with idiopathic short stature. Revista Portuguesa de Endocrinologia. Diabetes Metab 9(1):53–58Google Scholar
- Hiroguchi T (2006) Masculinization of female gastropod mollusks induced by organotin compounds, focusing on mechanism of actions of tributyltin and triphenyltin for development of imposex. Environ Sci 13(2):77–87Google Scholar
- Mortimer ST (2000) CASA- practical aspects. J Androl:515–524Google Scholar
- Smith KS (1981) 14-C-TPTH residue levels in milk and tissues of lactating dairy cows. Cannon Laboratories, USA. Report Project of 7489 [cited in FAO, 1991]Google Scholar
- Wang BA, Li M, Mu YM, Lu ZH, Li JY (2006) Effects of tributyltin chloride (TBT) and triphenyltin chloride (TPT) on rat testicular Leydig cells. Zhonghua Nan Ke Xue - Natl J Androl 12:516–519Google Scholar