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Environmental Science and Pollution Research

, Volume 26, Issue 9, pp 8821–8827 | Cite as

Relationship between arsenic accumulation in tissues and hematological parameters in mullet caught in Faro Lake: a preliminary study

  • Francesco FazioEmail author
  • Concetta Saoca
  • Vincenzo Ferrantelli
  • Gaetano Cammilleri
  • Gioele Capillo
  • Giuseppe Piccione
Research Article

Abstract

The authors investigated the arsenic (As) accumulation in different tissues (muscle, gill, liver, stomach, and intestine) and the possible correlation between tissue concentration and hematological parameters in mullet (Mugil cephalus Linnaeus, 1758) caught in Faro Lake (Messina, Sicily, Italy). On all fish, hematological analyses of blood samples, measurement of biometric indices, and the removal of the muscles, gills, liver, stomach, and intestine for the determination of arsenic concentration were performed. A hemogram was performed to find effects of arsenic concentration in tissues on hematological variables. One-way analysis of variance showed significant differences of arsenic concentration in different tissues, with higher values in the gill. The correlation between hematological parameters and tissue arsenic concentration showed a statistical significance for red blood cell (RBC), hemoglobin concentration (Hb), and hematocrit (Hct) with the liver As concentration. Biometric indices (weight, length, and fork length) showed a significant correlation with As concentration of the muscle and liver also. Our results indicate the role of some hematological parameters as biomarkers useful to monitoring anthropogenic load of arsenic in water and sediment, because variations of these parameters represent one of the effects that arsenic exposure can have on fish.

Keywords

Arsenic Bioaccumulation Blood parameters Environment Fish tissues Mugil cephalus 

Notes

Compliance with ethical standard

Conflict of interest

The authors declare that they have no conflict of interest.

Statement on welfare of animals

All experimental procedures were carried out in accordance with European legislation regarding the protection of animals used for scientific purposes (European Directive 2010/63). CISS ethical committee code 017/2017.

References

  1. Abernathy CO, Thomas DJ, Calderon L (2003) Health effects and risk assessment of arsenic. J Nutr 133:1536–1538CrossRefGoogle Scholar
  2. Adhikari S, Sarkar B, Chatterjee A, Mahapatra CT, Ayyappan S (2004) Effects of cypermethrin and carbofuran hematological parameters and prediction of their recovery in a freshwater teleost, Labeo rohita (Hamilton). Ecotoxicol Environ Saf 58:220–226CrossRefGoogle Scholar
  3. Akan JC, Salwa M, Yikala BS, Chellube ZM (2012) Study on the distribution of heavy metals in different tissues of fishes from river Benue in Vinikilang, Adamawa State, Nigeria. Br J Appl Sci Technol 2:311–333CrossRefGoogle Scholar
  4. Allen T, Singhal R, Rana SVS (2004) Resistance to oxidative stress in a freshwater fish Channa punctatus after exposure to inorganic arsenic. Biol Trace Elem Res 98:63–72CrossRefGoogle Scholar
  5. Allin CJ, Wilson RW (2000) Effects of pre-acclimation to aluminium on the physiology and swimming behavior of juvenile rainbow trout (Oncorhynchus mykiss) during a pulsed exposure. Aquat Toxicol 51:213–224CrossRefGoogle Scholar
  6. Bears H, Richards JG, Schulte PM (2006) Arsenic exposure alters hepatic arsenic species composition and stress-mediated gene expression in the common killifish (Fundulus heteroclitus). Aquat Toxicol 77:257–266CrossRefGoogle Scholar
  7. Bhattacharya A, Bhattacharya S (2007) Induction of oxidative stress by arsenic in Clarias batrachus: involvement of peroxisomes. Ecotoxicol Environ Saf 66:178–187CrossRefGoogle Scholar
  8. Bottari A, Bottari C, Carveni P, Giacobbe S, Spanò N (2005) Genesis and geomorphologic and ecological evolution of the Ganzirri salt marsh (Messina, Italy). Quat Int 140-141:150–158CrossRefGoogle Scholar
  9. Capillo G, Savoca S, Costa R, Sanfilippo M, Rizzo C, Lo Giudice A, Albergamo A, Rando R, Bartolomeo G, Spanò N, Faggio C (2018a) New insights into the culture method and antibacterial potential of Gracilaria gracilis. Mar Drugs 16:1–21CrossRefGoogle Scholar
  10. Capillo G, Panarello G, Savoca S, Sanfilippo M, Albano M, Li Volsi R, Consolo G, Spanò N (2018b) Intertidal ponds of Messina’s beachrock faunal assemblage, evaluation of ecosystem dynamics and communities’ interactions. Atti Della Accad Peloritana Dei Pericolanti - Cl Di Sci Fis Mat E Nat 96: in press Google Scholar
  11. Capillo G, Silvestro S, Sanfilippo M, Fiorino E, Giangrosso G, Ferrantelli V, Vazzana I, Faggio C (2018c) Assessment of electrolytes and metals profile of the Faro Lake (Capo Peloro Lagoon, Sicily, Italy) and its impact on Mytilus galloprovincialis. Chem Biodivers 15(5)Google Scholar
  12. Carvalho CS, Fernandes N (2006) Effect of temperature on copper toxicity and hematological responses in the neotropical fish, Prochilodus scrofa at low and high pH. Aquaculture 251:109–117CrossRefGoogle Scholar
  13. Chokkalingam K, Annamalai M, Satyanarayanan SK, Mathan R (2010) Toxicological effects of arsenate exposure on hematological, biochemical and liver transaminases activity in an Indian major carp, Catla catla. Food ChemToxicol 28:2848–2854Google Scholar
  14. Chowdhury MJ, Pane EF, Wood CM (2004) Physiological effects of dietary cadmium acclimation and waterborne cadmium challenge in rainbow trout: respiratory, ionoregulatory, and stress parameters. Comp Biochem Physiol C 139:163–173CrossRefGoogle Scholar
  15. Cockell KA, Hilton JW, Bettger WJ (1991) Chronic toxicity of dietary disodium arsenate heptahydrate to juvenile rainbow trout (Oncorhynchus mykiss). Arch Environ Contam Toxicol 21:518–552CrossRefGoogle Scholar
  16. Das S, Unni B, Bhattacharjee M, Wann SB, Rao PG (2012) Toxicological effects of arsenic exposure in a freshwater teleost fish, Channa punctatus. Afr J Biotechnol 11:4447–4454Google Scholar
  17. Datta S, Ghosh D, Saha DR, Bhattacharaya S, Mazumder S (2009) Chronic exposure to low concentration of arsenic is immunotoxic to fish: role of head kidney macrophages arsenic biomarkers of arsenic toxicity to Clarias batrachus. Aquat Toxicol 92:86–94CrossRefGoogle Scholar
  18. Ferreira M, Moradas-Ferreira P, Reis-Henriques MA (2005) Oxidative stress biomarkers in two resident species, mullet (Mugil cephalus) and flounder (Platichthys flesus), from a polluted site in River Douro Estuary, Portugal. Aquat Toxicol 71:39–48CrossRefGoogle Scholar
  19. Fazio F, Faggio C, Marafioti S, Torre A, Sanfilippo M, Piccione G (2012a) Comparative study of haematological profile on Gobius niger in two different habitat sites: Faro Lake and Tyrrhenian Sea. Cah Biol Mar 53:213–219Google Scholar
  20. Fazio F, Filiciotto F, Marafioti S, DiStefano V, Assenza A, Placenti F, Buscaino G, Piccione G, Mazzola S (2012b) Automatic analysis to assess haematological parameters in farmed gilthead sea bream (Sparus aurata Linnaeus, 1758). Mar Freshw Behav Physiol 45:63–65CrossRefGoogle Scholar
  21. Fazio F, Piccione G, Tribulato K, Ferrantelli V, Giangrosso G, Arfuso F, Faggio C (2014) Bioaccumulation of heavy metals in blood and tissue of striped mullet in two Italian lakes. J Aquat Anim Health 26:278–284CrossRefGoogle Scholar
  22. Filiciotto F, Fazio F, Marafioti S, Buscaino G, Maccarrone V, Faggio C (2012) Assessment of haematological parameters range values using an automatic method in European sea bass (Dicentrarchus labrax L.) Natura rerum 1:29–36Google Scholar
  23. Gaim K, Gebru G, Abba S (2015) The effect of arsenic on liver tissue of experimental animals (fishes and mice)-a review article. Int J Sci Res Publ 5:1–9Google Scholar
  24. Gernhöfer M, Pawert M, Scharmm M, Muller E, Triebskom R (2001) Ultrastructural biomarkers arsenic tool to characterize the health status of fish in contaminated. J Aquat Ecosysist Stress Recover 8:241–260CrossRefGoogle Scholar
  25. Gutierrez-Mejia E, Lares ML, Sosa-Nishizaki O (2009) Mercury and arsenic in muscle and liver of the golden cownose ray, Rhinoptera steindachneri, Evermann and Jenkins, 1891, from the upper Gulf of California, Mexico. Bull Environ Contam Toxicol 83:230–234CrossRefGoogle Scholar
  26. Hamdi M, Sanchez MA, Beene LC, Liu Q, Landfear SM, Rosen BP, Liu Z (2009) Arsenic transport by zebrafish aquaglyceroporins. BMC Mol Biol 10:104CrossRefGoogle Scholar
  27. Hughes MF, Beck BD, Chen Y, Lewis AS, Thomas DJ (2011) Arsenic exposure and toxicology: a historical perspective. Toxicol Sci 123:305–332CrossRefGoogle Scholar
  28. Ismail NM, Ali SE, Mohamed IK (2017) Biochemical and histological biomarker approaches in the assessment of the water pollution in some lined and unlined watercourses of Egypt. Int J Fish Aquat 5:288–296Google Scholar
  29. Italian Ministry of the Environment (2010) Ministerial decree 260 (2010): ministry of the environment and territorial protection and sea. Italian Ministry of the Environment, RomeGoogle Scholar
  30. Kumary B, Kumar V, Sinha AK, Ahsan J, Ghosh AK, Wang H, DeBoek G (2017) Toxicology of arsenic in fish and aquatic systems. Environ Chem Lett 15:43–64CrossRefGoogle Scholar
  31. Kumar R, Banerjee TK (2012) Impact of sodium arsenite on certain biomolecules of nutritional importance of the edible components of the economically important catfish C. batrachus (Linn.). Ecol Food Nutr 51:114–127CrossRefGoogle Scholar
  32. Lavanya S, Mathan R, Chokkalingam K, Annamalai M (2011) Hematological, biochemical and ionoregulatory responses of Indian major carp Catla catla during chronic sub lethal exposure to inorganic arsenic. Chemosphere 82:977–985CrossRefGoogle Scholar
  33. Licata P, Di Bella G, Dugo G, Naccari F (2003) Organochlorine pesticides, PCBs and heavy metals in tissues of the mullet Liza aurata in Lake Ganzirri and Straits of Messina (Sicily, Italy). Chemosphere 52:231–238CrossRefGoogle Scholar
  34. Licata P, Trombetta D, Cristani M, Martino D, Naccari F (2004) Organochlorine compounds and heavy metals in the soft tissue of the mussel Mytilus galloprovincialis collected from Lake Faro (Sicily, Italy). Environ Int 30:805–810CrossRefGoogle Scholar
  35. Manganaro A, Pulicanò G, Reale A, Sanfilippo M, Sarà G (2009) Filtration pressure by bivalves affects the trophic conditions in Mediterranean shallow ecosystems. Chem Ecol 25:467–478CrossRefGoogle Scholar
  36. Mdgela R, Myburgh J, Correia D, Braathen M, Ejobi F, Botha C, Sandvik M, Skaare JV (2006) Evaluation of the gill filament- based EROD assay in African sharptooth catfish (Clarias gariepinus ) arsenic a monitoring tool for waterborne PAH-type contaminants. Ecotoxicology 15:51–59CrossRefGoogle Scholar
  37. Odum WE (1970) Utilization of the direct grazing and plant detritus food chains by the striped Mullet Mugil cephalus. In. Berkeley A, Steele JJ (eds) Marine food chains. University of California, pp. 222–240Google Scholar
  38. Oladimeji AA, Qadri SU, DeFreitas AS (1984) Measuring the elimination of arsenic by the gills of rainbow trout (Salmo gairdneri) by using a two compartment respirometer. Bull Environ Contam Toxicol 32:661–668CrossRefGoogle Scholar
  39. Palaniappan PLRM, Vijayasundaram V (2009) The bioaccumulation of arsenic and the efficacy of Meso -2, 3-dimercaptosuccinic acid in the selected organ tissues of Labeo rohita fingerlings using inductively coupled plasma-optical emission spectrometry. World Appl Sci J 6:1247–1254Google Scholar
  40. Pedlar RM, Ptashynski MD, Wautier KG, Evans R, Baron CL, Klaverkamp JF (2002) The accumulation, distribution and toxicological effects of dietary arsenic exposure in lake white (Coregonus clupeaformis ) and lake trout ( Salvelinus namay-cush ). Comp Biochem Physiol C 131:73–91Google Scholar
  41. Rahman MAH, Hasegawa H (2012) Arsenic in freshwater systems: influence of eutrophication on occurrence, distribution, speciation, and bioaccumulation. Appl Geochem 27:304–314CrossRefGoogle Scholar
  42. Rahman MA, Hasegawa H, Lim RP (2012) Bioaccumulation, biotransformation and trophic transfer of arsenic in the aquatic food chain. Environ Res 116:118–135CrossRefGoogle Scholar
  43. Rossman TG (2003) Mechanism of arsenic carcinogenesis: an integrated approach. Mutat Res 533:37–65CrossRefGoogle Scholar
  44. Scoglio ME, Munao F, Di Pietro A, Picerno I (2000) Elementi in traccia` nel complesso lagunare Ganzirri-Faro. Rischio sanitario. (Trace elements in Ganzirri and Faro lakes). Ig Mod 114:243–260Google Scholar
  45. Scott GR, Sloman KA (2004) The effects of environmental pollutants on complex fish behaviour: integrating behavioural and physiological indicators of toxicity. Aquat Toxicol 68:369–392CrossRefGoogle Scholar
  46. Shah SL, Altindag A (2004) Hematological parameters on tench (Tinca tinca L.) after acute and chronic exposure to lethal and sublethal mercury treatments. Bull Environ Contam Toxicol 73:911–918CrossRefGoogle Scholar
  47. 1Spinelli A, Capillo G, Faggio C, Vitale D, Spanò N (2018) Returning of Hippocampus hippocampus (Linnaeus, 1758) (Syngnathidae) in the Faro Lake–oriented Natural Reserve of Capo Peloro, Italy. Natural Product Research.  https://doi.org/10.1080/14786419.2018.1490909
  48. Suhendrayatna Ohki A, Nakajima T, Maeda S (2002) Studies on the accumulation and transformation of arsenic in freshwater organisms II. Accumulation and transformation of arsenic compounds by Tilapia mossambica. Chemosphere 46:325–331CrossRefGoogle Scholar
  49. Taylor V, Goodale B, Raab A, Schwerdtle T, Reimer K, Conklin S, Karagas MR, Francesconi KA (2017) Human exposure to organic arsenic species from seafood. Sci Total Environ 580:266–282CrossRefGoogle Scholar
  50. Tisler T, Zagorc-Koncan J (2002) Acute and chronic toxicity of arsenic to some aquatic organisms. Bull Environ Contam Toxicol 69:421–429CrossRefGoogle Scholar
  51. Tripathi S, Sahu DB, Kumar R, Kumar A (2003) Effect of acute exposure of sodium arsenite (Na3AsO3) on some hematological parameters of Clarias batrachus (common Indian catfish) in vivo. Indian J Environ Health 45:183–188Google Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Veterinary SciencesPolo Universitario Annunziata, University of MessinaMessinaItaly
  2. 2.Experimental Zooprophylactic Institute of Sicily “A. Mirri”PalermoItaly
  3. 3.Department of Chemical, Biological, Pharmaceutical and Environmental SciencesMessinaItaly

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