Optimization of physical parameters for enhanced production of lipase from Staphylococcus hominis using response surface methodology

  • Ashis Ranjan Behera
  • Amrutha Veluppal
  • Kasturi DuttaEmail author
Appropriate Technologies to Combat Water Pollution


Lipase, a versatile hydrolytic enzyme, is gaining more importance in environmental applications such as treatment of oil and grease containing wastewater, pretreatment of solid waste/industrial wastewater for anaerobic treatment. In the present study, the attempts have been made to improve the production of lipase from Staphylococcus hominis MTCC 8980 by optimization of pH, temperature, and agitation speed in lab scale shake flasks culture. The experiments were designed using the full factorial central composite design of experiment. A total of 20 experiments were conducted, and the optimized pH, temperature, and agitation speed were found to be 7.9, 33.1 °C, and 178.4 rpm, respectively. The results of the analysis of variance (ANOVA) test revealed that the linear terms for temperature and agitation were significant (p value < 0.05). Interaction for pH and agitation speed was found to have a significant effect on lipase production from S. hominis MTCC 8980. A 150% increase in enzyme activity was observed under the optimized conditions with the maximum lipase activity of 1.82 U/ml. Further enhancement of enzyme activity can be expected from the optimization of medium components.


Lipase Optimization Central composite design Statistical analysis Staphylococcus hominis 


Compliance with ethical standards

We have followed the accepted principles of ethical and professional conduct.

Conflict of interest

The authors declare that they have no conflict of interest.

Human and animal rights and informed consent

The work did not involve human participants and/or animals.


  1. Basheer SM, Chellappan S, Beena PS, Sukumaran RK, Elyas KK, Chandrasekaran M (2011) Lipase from marine Aspergillus awamori BTMFW032 production, partial purification and application in oil effluent treatment. New Biotechnol 28(6):627–638CrossRefGoogle Scholar
  2. Bharathi D, Rajalakshmi G, Komathi S (2018) Optimization and production of lipase enzyme from bacterial strains isolated from petrol spilled soil. J King Saud Univ-SciGoogle Scholar
  3. Bosley JA, Clayton JC (1994) Blueprint for a lipase support use of hydrophobic controlled pore glasses as model systems. Biotechnol Bioeng 43:934–938CrossRefGoogle Scholar
  4. Charton E, Macrae AR (1992) Substrate specificities for lipases A and B from Geotrichum candidum CMICC 33546. Biochim Biophys Acta 1123:59–64CrossRefGoogle Scholar
  5. Chauhan M, Chauhan RS, Garlapati VK (2013) Modeling and optimization studies on a novel lipase production by Staphylococcus arlettae through submerged fermentation. Enzyme Res 2013:1–8CrossRefGoogle Scholar
  6. Colla LM, Primaz AL, Benedetti S, Loss RA, Lima MD, Reinehr CO, Bertolin TE, Costa JA (2016) Surface response methodology for the optimization of lipase production under submerged fermentation by filamentous fungi. Braz J Microbiol 47(2):461–467CrossRefGoogle Scholar
  7. Dharmsthiti S, Kuhasuntisuk B (1998) Lipase from Pseudomonas aeruginosa LP602 biochemical properties and application for wastewater treatment. J Ind Microbiol Biotechnol 21(1–2):75–80CrossRefGoogle Scholar
  8. Dutta K, Dasu VV, Hegde K (2013) Development of medium and kinetic modeling for enhanced production of cutinase from Pseudomonas cepacia NRRL B-2320. Adv Microbiol 3(6):479. CrossRefGoogle Scholar
  9. Javed S, Azeem F, Hussain S, Rasul I, Siddique MH, Riaz M, Nadeem H (2018) Bacterial lipases: a review on purification and characterization. Prog Biophys Mol Biol 132:23–34Google Scholar
  10. Kanmani P, Aravind J, Kumaresan K (2015) An insight into microbial lipases and their environmental facet. Int J Appl Biol Pharm 2(3):1147–1162Google Scholar
  11. Kaushik R, Saran S, Isar J, Saxena RK (2006) Statistical optimization of medium components and growth conditions by response surface methodology to enhance lipase production by Aspergillus carneus. J Mol Catal B Enzym 40(3–4):121–126CrossRefGoogle Scholar
  12. Larbidaouadi K, Benattouche Z, Abbouni B (2015) Screening selection identification production and optimization of bacterial lipase isolated from industrial rejection of gas station. Int J Biotechnol Allied Fields 3(9):146–153Google Scholar
  13. Liu CH, Lu WB, Chang JS (2006) Optimizing lipase production of Burkholderia sp. by response surface methodology. Process Biochem 41(9):1940–1944CrossRefGoogle Scholar
  14. Mahdi BA, Bhattacharya A, Gupta A (2012) Enhanced lipase production from Aeromonas sp. S1 using Sal deoiled seed cake as novel natural substrate for potential application in dairy wastewater treatment. J Chem Technol Biotechnol 87(3):418–426CrossRefGoogle Scholar
  15. Marimuthu K (2013) Isolation and characterization of Staphylococcus hominis JX961712 from oil contaminated soil. Int J Pharm Sci Res 7(3):252–256Google Scholar
  16. Momsia T, Momsia P (2013) A review on microbial lipase-versatile tool for industrial applications. Int J life Sci Biotechnol Pharma Res 2(4):2250–3137Google Scholar
  17. Moon HC, Song IS (2011) Enzymatic hydrolysis of foodwaste and methane production using UASB bioreactor. Int J Green Energy 8(3):361–371CrossRefGoogle Scholar
  18. de Morais WG Jr, Kamimura ES, Ribeiro EJ, Pessela BC, Cardoso VL, de Resende MM (2016) Optimization of the production and characterization of lipase from Candida rugosa and Geotrichum candidum in soybean molasses by submerged fermentation. Protein Expr Purif 123:26–34CrossRefGoogle Scholar
  19. Pignède G, Wang H, Fudalej F, Gaillardin C, Seman M, Nicaud JM (2000) Characterization of an extracellular lipase encoded by LIP2 in Yarrowia lipolytica. J Bacteriol 182(10):2802–2810CrossRefGoogle Scholar
  20. Prasad MP, Manjunath K (2011) Comparative study on biodegradation of lipid-rich wastewater using lipase producing bacterial species. Indian J Biotechnol 10(1):121–124Google Scholar
  21. Sharma A, Bardhan D, Patel R (2009) Optimization of physical parameters for lipase production from Arthrobacter sp. BGCC# 490. Indian J Biochem Biophys 46(2):178–183Google Scholar
  22. Singh R, Kumar M, Mittal A, Mehta PK (2016) Microbial enzymes: industrial progress in 21st century 3. Biotech 6(2):174Google Scholar
  23. Teng Y, Xu Y (2008) Culture condition improvement for whole-cell lipase production in submerged fermentation by Rhizopus chinensis using statistical method. Bioresour Technol 99(9):3900–3907CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Ashis Ranjan Behera
    • 1
  • Amrutha Veluppal
    • 1
  • Kasturi Dutta
    • 1
    Email author
  1. 1.Department of Biotechnology and Medical EngineeringNational Institute of TechnologyRourkelaIndia

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