Environmental Science and Pollution Research

, Volume 26, Issue 8, pp 7785–7792 | Cite as

High efficiency inactivation of microalgae in ballast water by a new proposed dual-wave UV-photocatalysis system (UVA/UVC-TiO2)

  • Zheng Lu
  • Kun Zhang
  • Xiaolei Liu
  • Yue ShiEmail author
Research Article


A new synergistic method was developed to inactivate marine microalgae using combined longwave ultraviolet (UVA) and shortwave ultraviolet (UVC)-photocatalysis (UVA/UVC-TiO2) technology. Five kinds of representative marine microalgae in three phyla were used as inactivating targets to examine the inactivation effect. Compared with the photocatalytic systems using UVA or UVC alone as the light source, the algae inactivation ratio in the newly developed system increased by 0.31 log or 0.19 log, and the chlorophyll a removal rate increased by 17.5% or 9.7%, respectively. Total suspended solids (TSS) of the seawater did not cause remarkable impact on the inactivation process, and the increase of UV radiation intensity improved the treatment effect significantly. Further, UVA/UVC-TiO2 treatment causes irreversible damage to microalgae cell membrane. The content of lipid peroxidation product malondialdehyde (MDA) increased rapidly within a short period of time, and a large number of proteins leaked out. The results of this study indicated that UVA/UVC-TiO2 was an effective method to solve the challenge of efficient inactivation of plankton in ballast water containing a high density of suspended matter.


Ballast water Microalgae Longwave ultraviolet Shortwave ultraviolet Photocatalysis 


Funding information

This research was financially supported by the National Key Research and Development Program of China (2017YFC1404605), the Natural Science Foundation of China (Grant No. 51579049 and 51509044), and the High Tech Ship Program.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Akram AC, Noman S, Moniri-Javid R, Gizicki JP, Reed EA, Singh SB, Basu AS, Banno F, Fujimoto M, Ram JL (2015) Development of an automated ballast water treatment verification system utilizing fluorescein diacetate hydrolysis as a measure of treatment efficacy. Water Res 70(1):404–413CrossRefGoogle Scholar
  2. American Public Health Association (1998) Standard methods for the examination of water and wastewater, twentieth ed. American Water Works Association; Water Pollution Control Federation, WashingtonGoogle Scholar
  3. Baker NR (2008) Chlorophyll fluorescence: a probe of photosynthesis in vivo. Annu Rev Plant Biol 59:89–113CrossRefGoogle Scholar
  4. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254CrossRefGoogle Scholar
  5. Cebi S, Celik M (2008) Assessment of technology options for ballast water treatment onboard merchant ships based on information axioms under fuzzy environment. In: Xia GP, Deng XQ (eds) Proceedings of the 38th international conference on computers and industrial engineering. Curran Associates Inc., Beijing, pp 652–657Google Scholar
  6. Chen C, Meng XY, Bai MD, Sun J, Meng FP (2014. Treatment system of ballast water in oceanic ships using hydroxyl radical (•OH) based on strong electric-field discharge. High Voltage Engineering 40(7):2238–2244Google Scholar
  7. de Lafontaine Y, Despatie S-P, Wiley C (2008) Effectiveness and potential toxicological impact of the PERACLEAN® ocean ballast water treatment technology. Ecotoxicol Environ Saf 71(2):355–369CrossRefGoogle Scholar
  8. Ding CS, Qin SL, Zheng YF, Miao J, Fu J (2010) Preparation and characterization of immobilized TiO2 and its photocatalytic activities. J China Univ Min Technol 39(3):431–436Google Scholar
  9. Du H, Zhang XF, Zhang ZT et al (2016) Input characteristics and risk analysis of ballast water in entry ships at China’s offshore sea area. Mar Sci Bull 35(1):112–120Google Scholar
  10. Esterbauer H, Cheeseman KH (1990) Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods Enzymol 186:407–421CrossRefGoogle Scholar
  11. Haaken D, Schmalz V, Dittmar T, Worch E (2013) Limits of UV disinfection: UV/electrolysis hybrid technology as a promising alternative for direct reuse of biologically treated wastewater. Water Supply Res Technol 62:442–451CrossRefGoogle Scholar
  12. Kim B, Kim D, Cho D, Cho S (2003) Bactericidal effect of TiO2 photocatalyst on selected food-borne pathogenic bacteria. Chemosphere 52:277–281CrossRefGoogle Scholar
  13. Kubacka A, Muñoz-Batista MJ, Ferrer M, Fernández-García M (2013) UV and visible light optimization of anatase TiO2 antimicrobial properties: surface deposition of metal and oxide (cu, Zn, ag) species. Appl Catal B Environ 140:680–690CrossRefGoogle Scholar
  14. Kühn KP, Chaberny IF, Massholder K, Stickler M, Benz VW, Sonntag HG, Erdinger L (2003) Disinfection of surfaces by photocatalytic oxidation with titanium dioxide and UVA light. Chemosphere 53:71–77CrossRefGoogle Scholar
  15. Liao XS, Wang X, Zhao KH, Zhou M (2007) Study on the influence of cyanobacterial growth by UV-C photocatalytic oxidation with Nanometric TiO2. J Wuhan Botan Res 25:457–461Google Scholar
  16. Lv BY, Cui YX, Tian W et al (2018) Abundances and profiles of antibiotic resistance genes as well as co-occurrences with human bacterial pathogens in ship ballast tank sediments from a shipyard in Jiangsu Province, China. Ecotoxicol Environ Saf 157(15):169–175CrossRefGoogle Scholar
  17. Mamlook R, Badran O, Abu-Khader MM, Holdo A, Dales J (2008) Fuzzy sets analysis for ballast water treatment systems: best available control technology. Clean Techn Environ Policy 10:397–407CrossRefGoogle Scholar
  18. Maness PC, Smolinski S, Blake DM, Huang Z, Wolfrum EJ, Jacoby WA (1999) Bactericidal activity of photocatalytic TiO2 reaction: toward an understanding of its killing mechanism. Appl Environ Microbiol 65(9):4094–4098Google Scholar
  19. Matafonova GG, Batoev VB, Linden KG (2012) Impact of scattering of UV radiation from an exciplex lamp on the efficacy of photocatalytic inactivation of Escherichia coli cells in water. J Appl Spectrosc 79(2):296–301CrossRefGoogle Scholar
  20. Naik K, Chatterjee A, Prakash H, Kowshik M (2013) Mesoporous TiO2 nanoparticles containing ag ion with excellent antimicrobial activity at remarkable low silver concentrations. J Biomed Nanotechnol 9(4):664–673CrossRefGoogle Scholar
  21. Rey A, Basurko OC, Rodríguez-Ezpeleta N (2018) The challenges and promises of genetic approaches for ballast water management. J Sea Res 133:134–145CrossRefGoogle Scholar
  22. Shie JL, Lee CH, Chiou CS, Chang CT, Chang CC, Chang CY (2008) Photodegradation kinetics of formaldehyde using light sources of UVA, UVC and UVLED in the presence of composed silver titanium oxide photocatalyst. J Hazard Mater 155(1–2):164–172CrossRefGoogle Scholar
  23. Stehouwer PP, van Slooten C, Peperzak L (2013) Microbial dynamics in acetate-enriched ballast water at different temperatures. Ecotoxicol Environ Saf 96(6):93–98CrossRefGoogle Scholar
  24. Sung-Suh HM, Choi JR, Hah HJ, Koo SM, Bae YC (2004) Comparison of ag deposition effects on the photocatalytic activity of nanoparticulate TiO2 under visible and UV light irradiation. J Photochem Photobiol A Chem 163(1):37–44CrossRefGoogle Scholar
  25. Suri RPS, Thornton HM, Muruganandham M (2012) Disinfection of water using Pt- and ag-doped TiO2 photocatalysts. Environ Technol 33:1651–1659CrossRefGoogle Scholar
  26. Tao P, Xu Y, Zhou Y, Song C, Shao M, Wang T (2017) Coal-based carbon membrane coupled with electrochemical oxidation process for the enhanced microalgae removal from simulated ballast water. Water Air Soil Pollut 228(11):421CrossRefGoogle Scholar
  27. United States Environmental Protection Agency (2010) Environmental technology verification program (ETV) generic protocol for the verification of ballast water treatment technology, version version 5.1, Report number EPA/600/R-10/146. Washington, DC, USAGoogle Scholar
  28. Wang WJ, Huang GC, Yu JC, Wong PK (2015) Advances in photocatalytic disinfection of bacteria: development of photocatalysts and mechanisms. J Environ Sci 34(1):232–247CrossRefGoogle Scholar
  29. Zacarías SM, Vaccari MC, Alfano OM, Irazoqui HA, Imoberdorf GE (2010) Effect of the radiation flux on the photocatalytic inactivation of spores of Bacillus subtilis. J Photochem Photobiol A Chem 214:171–180CrossRefGoogle Scholar
  30. Zacchini M, de Agazio M (2004) Spread of oxidative damage and antioxidative response through cell layers of tobacco callus after UV-C treatment. Plant Physiol Biochem 42:445–450CrossRefGoogle Scholar
  31. Zhao J, Krishna V, Hua B, Moudgil B, Koopman B (2009) Effect of UVA irradiance on photocatalytic and UVA inactivation of Bacillus cereus spores. J Photochem Photobiol B Biol 94:96–100CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Power and Energy EngineeringHarbin Engineering UniversityHarbinChina
  2. 2.School of Economics and ManagementHarbin Engineering UniversityHarbinChina

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