Environmental Science and Pollution Research

, Volume 26, Issue 8, pp 7863–7872 | Cite as

Bio-removal of cadmium from aqueous solutions by filamentous fungi: Trichoderma spp. and Piriformospora indica

  • Yasser YaghoubianEmail author
  • Seyed Ataollah Siadat
  • Mohammad Reza Moradi Telavat
  • Hemmatollah Pirdashti
  • Iraj Yaghoubian
Research Article


Six strains of Trichoderma spp. and Piriformospora indica have been studied for cadmium tolerance and bioaccumulation capacities by the poisoned food technique. A quantitative assay for Trichoderma spp. and P. indica was conducted in broth cultures supplemented with different cadmium concentrations (0–500 mg/l). In addition, the growth pattern of P. indica was determined by growing the fungus in a solid medium amended with eight concentrations of cadmium (0–200 mg/l). Generally, an increasing cadmium gradient in the culture medium suppressed the ability of fungi for cadmium accumulation. However, a negative relation was observed between the biomass production of fungi and cadmium uptake (q: mg/g biomass). Results showed that Trichoderma spp., especially T. simmonsii [UTFC 10063], are tolerant to cadmium toxicity and have a high ability to cadmium bioaccumulation. The biomass production of T. simmonsii [UTFC 10063] was significantly stimulated and increased by 46.1% when cadmium concentration increased from 0 to 125 mg/l. Moreover, 91.7 and 31.2% of cadmium removal was observed at 10 and 500 mg/l of the cadmium concentration, respectively. P. indica, however, showed a lower tolerance and removal efficiency for cadmium as compared with Trichoderma spp. Therefore, Trichoderma spp., especially T. simmonsii [UTFC 10063], can be exploited as potent bio-removal agents in cadmium-polluted aqueous solutions.

Graphical abstract


Bioaccumulation efficacy Cadmium toxicity Growth pattern Removal capacities Spore germination 



We thank Dr. M.A. Tajick Ghanbari and Dr. V. Babaeizad for providing the fungal strains and Dr. M. Najafi and Dr. A. Alizadeh for technical assistance.

Funding information

The authors wish to acknowledge Ramin Agricultural and Natural Resources University (Ahvaz, Iran), Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), and Sari Agricultural Sciences and Natural Resources University (Sari, Iran) for financial support of this study.

Supplementary material

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ESM 1 (XLSX 22 kb)
11356_2019_4255_MOESM2_ESM.xlsx (19 kb)
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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Genetics and Agricultural Biotechnology Institute of TabarestanSari Agricultural Sciences and Natural Resources UniversitySariIran
  2. 2.Department of Agronomy and Plant Breeding, Faculty of AgricultureAgricultural Sciences and Natural Resources University of KhuzestanAhvazIran
  3. 3.Department of Agronomy, Faculty of Crop ScienceSari Agricultural Sciences and Natural Resources UniversitySariIran
  4. 4.Department of Agronomy, Faculty of AgricultureTarbiat Modares UniversityTehranIran

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