Influence of nutrient formulations on growth, lipid yield, carbon partitioning and biodiesel quality potential of Botryococcus sp. and Chlorella sp.
The study was conducted to analyse the influence of three nutrient formulations, namely BG-11 medium, BBM and TAP medium, on growth potential and lipid yield of two microalgal genera (Botryococcus sp. and Chlorella sp.) and to study the roles of N, P and other major nutrients. The study focussed on the general patterns of starch and lipid synthesis and storage and to further assess how photosynthetic carbon partitioning into starch and lipid is altered by conditions in growth media such as N and C presence as seen in BG11 medium which are known to induce neutral lipid production and the lack of it in BBM and TAP medium. BG-11 medium performed better as compared to BBM and TAP medium in terms of biomass productivity and lipid yield. The lipid yield was highest in Botryococcus sp. (63.03% dry wt.) and Chlorella sp. (50.27% dry wt.) at 30th day of incubation. Mean biomass productivity was highest for Botryococcus in BBM medium (6.14 mg/L/day) and for Chlorella in BG-11 medium (4.97 mg/L/day). Mean lipid productivity (50.78% and 39.36%) was highest in BG11 medium for both Botryococcus and Chlorella species, respectively. A sharp decline in sugar content was observed in the late stationary phase of growth from 30th day to 45th day. Fatty acid methyl ester (FAME) profile of the extracted lipids showed predominantly oleic acid, followed by palmitic acid and stearic acid in both the strains when grown in BG-11 medium. The other biodiesel quality parameters were in accordance with the international standards. A complex relationship was found between chemical composition and biodiesel properties. Proximity analysis indicated that the fuel properties of biodiesels are determined by a number of parameters and by the combination of different chemical compositions. The results provide an insight into organic carbon partitioning into lipid compounds and how the organism’s lipid metabolism changes due to N-deplete culturing in TAP medium and inorganic carbon source availability as seen in BG-11 and BBM medium.
KeywordsLipid Nutrient media Biodiesel FAMEs Biomass Microalgae
The authors are grateful to Director, IARI, New Delhi, for essential facilities.
The study is financially supported by the Department of Biotechnology, Govt. of India under Indo-Denmark Collaborative Project.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- DuBois M, Gilles KA., Hamilton JK., Rebers PA, Smith F (1956) Colorimetric Method for Determination of Sugars and Related Substances. Anal Chem 28(3):350–356Google Scholar
- El-Shimi HI et al. (2013) Biodiesel production from Spirulina-platensis microalgae by in-situ transesterification process. Journal of Sustainable Bioenergy Systems 3(3):224Google Scholar
- Franco-Navarro JD, Brumós J, Rosales MA, Cubero-Font P, Talón M, and Colmenero-Flores JM (2015) Chloride regulates leaf cell size and water relations in tobacco plants. J Exp Bot 67(3):873-891.Google Scholar
- Frankel EN (1998) Lipid oxidation. Dundee: The Oily Press. Vol 10Google Scholar
- Fung KS, Liew EWT, Ngu HLN (2013) Optimization of nutrient media composition for microalgae biomass production using central composite design. Chemeca 2013: Challenging Tomorrow, p.278Google Scholar
- Hakkalin NLS, Paz AP, Aranda DAG, Moraes LMP (2014) Enhancement of cell growth and lipid content of a freshwater microalga Scenedesmus sp. by optimizing nitrogen, phosphorus and vitamin concentrations for biodiesel production. Nat Sci 6:1044–1054Google Scholar
- Held P, Raymond K, (2011) Determination of algal cell lipids using Nile red—using microplates to monitor neutral lipids in Chlorella vulgaris Google Scholar
- Hu Q (2004) Environmental effects on cell composition. In: Richmond, A. (Ed.), Handbook of Microalgal Culture: Biotechnology and Applied Phycology. Blackwell Science, Victoria, pp. 83–93Google Scholar
- Nelson DL, Cox MM (2008) Lipid biosynthesis. In: Principles of biochemistry, 4th edn. W. H. Freeman and Company, New York, pp 805–845Google Scholar
- Raven JA (2016) Chloride: essential micronutrient and multifunctional beneficial ion. J Exp bot 68(3):359–367.Google Scholar
- Ruangsomboon S (2015) Effects of different media and nitrogen sources and levels on growth and lipid of green microalga Botryococcus braunii KMITL and its biodiesel properties based on fatty acid composition. Bioresour technol 191:377–384Google Scholar
- Sarpal AS, Costa ICR, Teixeira CMLL, Filocomo D, Candido R et al (2016) Investigation of biodiesel potential of biomasses of Microalgaes chlorella, spirulina and tetraselmis by NMR and GC-MS techniques. J Biotechnol Biomater 6:220Google Scholar
- Siaut M, Cuine S, Cagnon C, Fessler B, Naguyen M, Carrier P, Beyly A, Beisson F, Triantaphylidès C, Li-Beisson Y, Gilles P (2011) Oil accumulation in the model green alga Chlamydomonas reinhardtii: characterization, variability between common laboratory strains and relationship with starch reserves. BMC Biotechnol 11:7CrossRefGoogle Scholar
- Stanier RY, Kunisawa R, Mandel M, Cohen-Bazire G (1971) Purification and properties of unicellular blue-green algae (order Chroococcales). Bacteriol Rev 35:171–205Google Scholar
- Work VH, Radakovits R, Jinkerson RE, Meuser JE, Elliott LG, Vinyard DJ, Laurens LML, Dismukes GC, Posewitz M (2010) Increased lipid accumulation in the Chlamydomonas reinhardtii sta7-10 starchless isoamylase mutant and increased carbohydrate synthesis in complemented strain. Eukaryot Cell 9:1251–1269. https://doi.org/10.1128/EC.00075-10 CrossRefGoogle Scholar