Environmental Science and Pollution Research

, Volume 26, Issue 10, pp 10306–10318 | Cite as

Toxicological effects benzotriazole to the marine scallop Chlamys nobilis: a 2-month exposure study

  • Tang-tian He
  • Tao Zhang
  • Shi-bo Liu
  • Jing-chun Shi
  • Yi-sheng Huang
  • Huai-ping Zheng
  • Wen-hua LiuEmail author
Research Article


Benzotriazole and its associated derivatives (BTs) are widely used as ultraviolet stabilizers and corrosion inhibitors. They have been extensively found in marine environments and are bioaccumulative through the food chain. However, the toxicities of BTs to marine organisms are seldom identified and no assessment has been conducted for filter-feeding bivalves. In this study, a marine scallop Chlamys nobilis was exposed to 0, 0.01, 0.1, and 1.0 mg/L of BT for 60 days. Effects of BT on endocrine system, cytochrome P450 activity, antioxidant activity, and neural activity of C. nobilis were examined. The results showed that BT exerted significant estrogenic effects on both male and female scallops and inhibited EROD activities of C. nobilis even at 0.01 mg/L level. BT at ≥ 0.01 mg/L levels also caused significant oxidative stress on C. nobilis. Moreover, most of the adverse effects of BT to C. nobilis were found from day 35 and 0.01 mg/L was the lowest concentration with observed effects, showing the long-term toxic effects of BT to C. nobilis. Thus, the adverse effects of BT and its derivatives to marine benthic communities deserve more attention in future research.


Benzotriazole Chlamys nobilis Vitellogenin EROD Oxidative stress 



The help of Yuelin Li in scallop collection and laboratory work are greatly appreciated. Dr. Ping Li, Cui Guo, Diwen Wang, Huibin Cao, Huihui Liu, and Tong Chen from Shantou University also provided invaluable assistance and guidance to this study.

Funding information

This work was supported by grants from the Marine Public Welfare Project of China (no. 201005012) and 211 Project of Guangdong Province (no. 20091686).

Supplementary material

11356_2019_4201_MOESM1_ESM.docx (854 kb)
ESM 1 (DOCX 854 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Tang-tian He
    • 1
    • 2
  • Tao Zhang
    • 1
  • Shi-bo Liu
    • 1
    • 3
  • Jing-chun Shi
    • 1
  • Yi-sheng Huang
    • 1
  • Huai-ping Zheng
    • 1
  • Wen-hua Liu
    • 1
    Email author
  1. 1.Marine Biology InstituteShantou UniversityShantouPeople’s Republic of China
  2. 2.State Key Laboratory in Marine PollutionCity University of Hong KongKowloonChina
  3. 3.Songliao River Basin Water Environment Monitoring CenterJilinPeople’s Republic of China

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