Toxicological effects benzotriazole to the marine scallop Chlamys nobilis: a 2-month exposure study
Benzotriazole and its associated derivatives (BTs) are widely used as ultraviolet stabilizers and corrosion inhibitors. They have been extensively found in marine environments and are bioaccumulative through the food chain. However, the toxicities of BTs to marine organisms are seldom identified and no assessment has been conducted for filter-feeding bivalves. In this study, a marine scallop Chlamys nobilis was exposed to 0, 0.01, 0.1, and 1.0 mg/L of BT for 60 days. Effects of BT on endocrine system, cytochrome P450 activity, antioxidant activity, and neural activity of C. nobilis were examined. The results showed that BT exerted significant estrogenic effects on both male and female scallops and inhibited EROD activities of C. nobilis even at 0.01 mg/L level. BT at ≥ 0.01 mg/L levels also caused significant oxidative stress on C. nobilis. Moreover, most of the adverse effects of BT to C. nobilis were found from day 35 and 0.01 mg/L was the lowest concentration with observed effects, showing the long-term toxic effects of BT to C. nobilis. Thus, the adverse effects of BT and its derivatives to marine benthic communities deserve more attention in future research.
KeywordsBenzotriazole Chlamys nobilis Vitellogenin EROD Oxidative stress
The help of Yuelin Li in scallop collection and laboratory work are greatly appreciated. Dr. Ping Li, Cui Guo, Diwen Wang, Huibin Cao, Huihui Liu, and Tong Chen from Shantou University also provided invaluable assistance and guidance to this study.
This work was supported by grants from the Marine Public Welfare Project of China (no. 201005012) and 211 Project of Guangdong Province (no. 20091686).
- Breedveld GD, Roseth R, Hem L, Sparrevik M (2002) Triazoles in the terrestrial environment. 20001103-1 final report. Norwegian geotechnical institute, OsloGoogle Scholar
- Coburn C, Hudgens R, Mullen M (1999) Environmental effects of engine coolant additives. SAE technical paper 1999-01-0137. https://doi.org/10.4271/1999-01-0137
- Fent K, Woodin BR, Stegeman JJ (1998) Effects of triphenyltin and other organotins on hepatic monooxygenase system in fish. Comp Biochem Physiol Part C Toxicol Pharmacol 121:277–288Google Scholar
- Health Council of The Netherlands (2000) Dutch expert committee on occupational standards (DECOS): 1,2,3-Benzotriazole. Health Council of the Netherlands, the Hague (publication no. 2000/14OSH)Google Scholar
- Konuma S, Ogawa A, Masunaga S, Nakamura Y (2009) UV filters in seawater and sediment: partitioning and horizontal distributions on the coastal shoreline in and around the Tokyo Bay. In: 18th Symposium on Environmental Chemistry, pp 448–449Google Scholar
- Montesdeoca-Esponda S, Sosa-Ferrera Z, Santana-Rodríguez JJ (2012) On-line solid-phase extraction coupled to ultra-performance liquid chromatography with tandem mass spectrometry detection for the determination of benzotriazole UV stabilizers in coastal marine and wastewater samples. Anal Bioanal Chem 403:867–876CrossRefGoogle Scholar
- Nakata H, Shinohara RI, Nakazawa Y, Isobe T, Sudaryanto A, Subramanian A, Tanabe S, Zakaria MP, Zheng GJ, Lam PKS, Kim EY, Min BY, We SU, Viet PH, Tana TS, Prudente M, Frank D, Lauenstein G, Kannan K (2012) Asia-Pacific mussel watch for emerging pollutants: distribution of synthetic musks and benzotriazole UV stabilizers in Asian and US coastal waters. Mar Pollut Bull 64:2211–2218CrossRefGoogle Scholar
- Song S, Ruan T, Wang T, Liu R, Jiang G (2014) Occurrence and removal of benzotriazole ultraviolet stabilizers in a wastewater treatment plant in China. Environ Sci: Process Impacts 16:1076–1082Google Scholar
- Vaccaro E, Meucci V, Intorre L, Soldani G, Di Bello D, Longo V, Gervasi PG, Pretti C (2005) Effects of 17 beta-estradiol, 4-nonylphenol and PCB 126 on the estrogenic activity and phase 1 and 2 biotransformation enzymes in male sea bass (Dicentrarchus labrax). Aquat Toxicol 75:293–305CrossRefGoogle Scholar