Effects of antibiotics on nitrogen uptake of four wetland plant species grown under hydroponic culture Research Article First Online: 14 February 2019 Abstract
To investigate the effects of antibiotics on nitrogen removal and uptake by wetland plants, four typical macrophyte species,
Cyperus alternifolius L., Typha angustifolia L., Lythrum salicaria L., and Acorus calamus L., were grown in hydroponic cultivation systems and fed wastewater polluted with 10 μg L −1 Ofloxacin (OFL) and Tetracycline (TET). Biomass production, nitrogen mass concentration, chlorophyll content, root exudates, and nitrogen removal efficiency of hydroponic cultivation were investigated. The results indicated that in all hydroponic systems, NH 4 +–N was entirely removed from the hydroponic substrate within 1 day and plant nitrogen accumulation was the main role of the removed NO 3 −. OFL and TET stimulated the accumulation of biomass and nitrogen of A. calamus but significantly inhibited the NO 3 −–N removal ability of L. salicaria (98.6 to 76.2%) and T. augustifolia (84.3 to 40.2%). This indicates that A. calamus may be a good choice for nitrogen uptake in wetlands contaminated with antibiotics. OFL and TET improved the concentrations of total organic carbon (TOC), total nitrogen (TN), organic acid, and soluble sugars in root exudates, especially for oxalic acid. Considering the significant correlation between TOC of root exudates and nitrogen removal efficiency, the TOC of root exudates may be an important index for choosing macrophytes to maintain nitrogen removal ability in wetlands contaminated with antibiotics. Keywords Antibiotics Macrophyte species Nitrogen Oxalic acid Plant uptake Root exudates
Responsible editor: Philippe Garrigues
Notes Funding information
The authors received financial support from the Major Science and Technology Program for Water Pollution Control and Treatment of China (NO. 2017ZX07203-005) (NO. 2012ZX07105-003).
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Ahmed MBM, Rajapaksha AU, Lim JE et al (2015) Distribution and accumulative pattern of tetracyclines and sulfonamides in edible vegetables of cucumber, tomato, and lettuce. J Agric Food Chem 63:398–405.
https://doi.org/10.1021/jf5034637 CrossRef Google Scholar
Badri DV, Vivanco JM (2009) Regulation and function of root exudates. Plant, Cell Environ 32:666–681.
https://doi.org/10.1111/j.1365-3040.2009.01926.x CrossRef Google Scholar
Bloom AJ, Sukrapanna SS, Warner RL (1992) Root Respiration Associated With Ammonium and Nitrate Absorption and Assimilation By Barley. Plant Physiol 99:1294–1301.
https://doi.org/10.1104/pp.99.4.1294 CrossRef Google Scholar
Borin M, Salvato M (2012) Effects of five macrophytes on nitrogen remediation and mass balance in wetland mesocosms. Ecol Eng 46:34–42.
https://doi.org/10.1016/j.ecoleng.2012.04.034 CrossRef Google Scholar
Caban JR, Kuppusamy S, Kim JH et al (2018) Hairy Vetch Incorporated as Green Manure Inhibits Sulfathiazole Uptake by Lettuce in Soil. Water Air Soil Pollut 229:104.
https://doi.org/10.1007/s11270-018-3710-8 CrossRef Google Scholar
Chen J, Xu H, Sun Y et al (2016) Interspecific differences in growth response and tolerance to the antibiotic sulfadiazine in ten clonal wetland plants in South China. Sci Total Environ 543:197–205.
https://doi.org/10.1016/j.scitotenv.2015.11.015 CrossRef Google Scholar
Chung AKC, Wu Y, Tam NFY, Wong MH (2008) Nitrogen and phosphate mass balance in a sub-surface flow constructed wetland for treating municipal wastewater. Ecol Eng 32:81–89.
https://doi.org/10.1016/j.ecoleng.2007.09.007 CrossRef Google Scholar
Cruz-Ortega R, Ayala-Cordero G, Anaya AL (2002) Allelochemical stress produced by the aqueous leachate of Callicarpa acuminata: Effects on roots of bean, maize, and tomato. Physiol Plant 116:20–27.
https://doi.org/10.1034/j.1399-3054.2002.1160103.x CrossRef Google Scholar
Deng H, Ye ZH, Wong MH (2004) Accumulation of lead, zinc, copper and cadmium by 12 wetland plant species thriving in metal-contaminated sites in China. Environ Pollut 132:29–40.
https://doi.org/10.1016/j.envpol.2004.03.030 CrossRef Google Scholar
Gujarathi NP, Haney BJ, Park HJ et al (2005) Hairy roots of Helianthus annuus: A model system to study phytoremediation of tetracycline and oxytetracycline. Biotechnol Prog 21:775–780.
https://doi.org/10.1021/bp0496225 CrossRef Google Scholar
He S, Dong D, Zhang X et al (2018) Occurrence and ecological risk assessment of 22 emerging contaminants in the Jilin Songhua River (Northeast China). Environ Sci Pollut Res 25:24003–24012.
https://doi.org/10.1007/s11356-018-2459-3 CrossRef Google Scholar
Hillis DG, Fletcher J, Solomon KR, Sibley PK (2011) Effects of ten antibiotics on seed germination and root elongation in three plant species. Arch Environ Contam Toxicol 60:220–232.
https://doi.org/10.1007/s00244-010-9624-0 CrossRef Google Scholar
Huang L, Gao X, Liu M et al (2012) Correlation among soil microorganisms, soil enzyme activities, and removal rates of pollutants in three constructed wetlands purifying micro-polluted river water. Ecol Eng 46:98–106.
https://doi.org/10.1016/j.ecoleng.2012.06.004 CrossRef Google Scholar
Huang X, Lakso AN, Eissenstat DM (2005) Interactive effects of soil temperature and moisture on Concord grape root respiration. J Exp Bot 56:2651–2660.
https://doi.org/10.1093/jxb/eri258 CrossRef Google Scholar
Huang X, Liu C, Li K et al (2015) Performance of vertical up-flow constructed wetlands on swine wastewater containing tetracyclines and tet genes. Water Res 70:109–117.
https://doi.org/10.1016/j.watres.2014.11.048 CrossRef Google Scholar
Huang XD, El-Alawi Y, Penrose DM et al (2004) A multi-process phytoremediation system for removal of polycyclic aromatic hydrocarbons from contaminated soils. Environ Pollut 130:465–476.
https://doi.org/10.1016/j.envpol.2003.09.031 CrossRef Google Scholar
Kayashima T (2002) Oxalic acid is available as a natural antioxidant in some systems. Biochim Biophys Acta - Gen Subj 1573:1–3.
https://doi.org/10.1016/S0304-4165(02)00338-0 CrossRef Google Scholar
Leto C, Tuttolomondo T, La Bella S et al (2013) Effects of plant species in a horizontal subsurface flow constructed wetland - phytoremediation of treated urban wastewater with Cyperus alternifolius L. and Typha latifolia L. in the West of Sicily (Italy). Ecol Eng 61:282–291.
https://doi.org/10.1016/j.ecoleng.2013.09.014 CrossRef Google Scholar
Lin YF, Jing SR, Wang TW, Lee DY (2002) Effects of macrophytes and external carbon sources on nitrate removal from groundwater in constructed wetlands. Environ Pollut 119:413–420.
https://doi.org/10.1016/S0269-7491(01)00299-8 CrossRef Google Scholar
Liu D, Ge Y, Chang J et al (2009) Constructed wetlands in China: Recent developments and future challenges. Front Ecol Environ 7:261–268.
https://doi.org/10.1890/070110 CrossRef Google Scholar
Liu L, Liu C, Zheng J et al (2013a) Chemosphere Elimination of veterinary antibiotics and antibiotic resistance genes from swine wastewater in the vertical flow constructed wetlands. Chemosphere 91:1088–1093.
https://doi.org/10.1016/j.chemosphere.2013.01.007 CrossRef Google Scholar
Liu L, Liu YH, Liu CX et al (2013b) Potential effect and accumulation of veterinary antibiotics in Phragmites australis under hydroponic conditions. Ecol Eng 53:138–143.
https://doi.org/10.1016/j.ecoleng.2012.12.033 CrossRef Google Scholar
Liu X, Zhang H, Li L et al (2016) Levels, distributions and sources of veterinary antibiotics in the sediments of the Bohai Sea in China and surrounding estuaries. Mar Pollut Bull 109:597–602.
https://doi.org/10.1016/j.marpolbul.2016.05.033 CrossRef Google Scholar
Ma T, Zhou L, Chen L et al (2016) Oxytetracycline Toxicity and Its Effect on Phytoremediation by Sedum plumbizincicola and Medicago sativa in Metal-Contaminated Soil. J Agric Food Chem 64:8045–8053.
https://doi.org/10.1021/acs.jafc.6b02140 CrossRef Google Scholar
McClure PR, Kochian LV, Spanswick RM, Shaff JE (1990) Evidence for cotransport of nitrate and protons in maize roots: I. Effects of nitrate on the membrane potential. Plant Physiol 93:281–289.
https://doi.org/10.1104/pp.93.1.281 CrossRef Google Scholar
Michelini L, La Rocca N, Rascio N, Ghisi R (2013) Structural and functional alterations induced by two sulfonamide antibiotics on barley plants. Plant Physiol Biochem 67:55–62.
https://doi.org/10.1016/j.plaphy.2013.02.027 CrossRef Google Scholar
Michelini L, Reichel R, Werner W et al (2012) Sulfadiazine uptake and effects on salix fragilis l. and zea mays l. plants. Water Air Soil Pollut 223:5243–5257.
https://doi.org/10.1007/s11270-012-1275-5 CrossRef Google Scholar
Mikes O, Trapp S (2010) Acute toxicity of the dissociating veterinary antibiotics trimethoprim to willow trees at varying pH. Bull Environ Contam Toxicol 85:556–561.
https://doi.org/10.1007/s00128-010-0150-6 CrossRef Google Scholar
Miller AJ, Smith SJ (1996) Nitrate transport and compartmentation in cereal root cells. J Exp Bot 47:843–854.
https://doi.org/10.1093/jxb/47.7.843 CrossRef Google Scholar
Naamala J, Jaiswal SK, Dakora FD (2016) Antibiotics Resistance in Rhizobium: Type, Process, Mechanism and Benefit for Agriculture. Curr Microbiol 72:804–816.
https://doi.org/10.1007/s00284-016-1005-0 CrossRef Google Scholar
Rewald B, Kunze ME, Godbold DL (2016) NH4: NO3nutrition influence on biomass productivity and root respiration of poplar and willow clones. GCB Bioenergy 8:51–58.
https://doi.org/10.1111/gcbb.12224 CrossRef Google Scholar
Song C, Zhang C, Kamira B et al (2017) Occurrence and human dietary assessment of fluoroquinolones antibiotics in cultured fish around tai lake, China. Environ Sci Pollut Res 36:2899–2905.
https://doi.org/10.1002/etc.3876 Google Scholar
Sun L, Lu Y, Kronzucker HJ, Shi W (2016) Quantification and enzyme targets of fatty acid amides from duckweed root exudates involved in the stimulation of denitrification. J Plant Physiol 198:81–88.
https://doi.org/10.1016/j.jplph.2016.04.010 CrossRef Google Scholar
Tanner CC (2001) Growth and nutrient dynamics of soft-stem bulrush in constructed wetlands treating nutrient- rich wastewaters. Wetl Ecol Manag 9:49–73
CrossRef Google Scholar
Vymazal J (2010) Constructed Wetlands in the Czech Republic: 20 Years of Experience (Water and Nutrient Management in Natural and Constructed Wetlands). Springer, Dordrecht
Vymazal J (2007) Removal of nutrients in various types of constructed wetlands. Sci Total Environ 380:48–65.
https://doi.org/10.1016/j.scitotenv.2006.09.014 CrossRef Google Scholar
Wang L, Gan H, Wang F et al (2010) Characteristic analysis of plants for the removal of nutrients from a constructed wetland using reclaimed water. Clean - Soil, Air, Water 38:35–43.
https://doi.org/10.1002/clen.200900162 CrossRef Google Scholar
Wang W, Wang H, Zhang W et al (2017) Occurrence, distribution, and risk assessment of antibiotics in the Songhua River in China. Environ Sci Pollut Res 24:19282–19292.
https://doi.org/10.1007/s11356-017-9471-x CrossRef Google Scholar
Wang Z, Liu C, Liao J et al (2014) Nitrogen removal and N
O emission in subsurface vertical fl ow constructed wetland treating swine wastewater : Effect of shunt ratio. Ecol Eng 73:446–453.
https://doi.org/10.1016/j.ecoleng.2014.09.109 CrossRef Google Scholar
Weir TL, Bais HP, Stull VJ et al (2006) Oxalate contributes to the resistance of Gaillardia grandiflora and Lupinus sericeus to a phytotoxin produced by Centaurea maculosa. Planta 223:785–795.
https://doi.org/10.1007/s00425-005-0192-x CrossRef Google Scholar
Wu H, Wang X, He X et al (2017) Effects of root exudates on denitrifier gene abundance, community structure and activity in a micro-polluted constructed wetland. Sci Total Environ 598:697–703.
https://doi.org/10.1016/j.scitotenv.2017.04.150 CrossRef Google Scholar
Wu H, Xu K, He X, Wang X (2016) Removal of Nitrogen by Three Plant Species in Hydroponic Culture: Plant Uptake and Microbial Degradation. Water Air Soil Pollut 227:324.
https://doi.org/10.1007/s11270-016-3036-3 CrossRef Google Scholar
Zacchini M, De Agazio M (2004) Spread of oxidative damage and antioxidative response through cell layers of tobacco callus after UV-C treatment. Plant Physiol Biochem 42:445–450.
https://doi.org/10.1016/j.plaphy.2004.03.007 CrossRef Google Scholar
Zhao S, Liu X, Cheng D et al (2016) Temporal–spatial variation and partitioning prediction of antibiotics in surface water and sediments from the intertidal zones of the Yellow River Delta, China. Sci Total Environ 569–570:1350–1358.
https://doi.org/10.1016/j.scitotenv.2016.06.216 CrossRef Google Scholar Copyright information
© Springer-Verlag GmbH Germany, part of Springer Nature 2019