Advertisement

Physiological traits of land snails Theba pisana as simple endpoints to assess the exposure to some pollutants

  • Kawther S. El-GendyEmail author
  • Mohamed A. Radwan
  • Amira F. Gad
  • Awatef E. Khamis
  • El-Sayed H. Eshra
Research Article
  • 27 Downloads

Abstract

In the current study, the toxicity bioassay of three pollutants abamectin (ABM), thiamethoxam (TMX), and acrylamide (ACR) against land snails Theba pisana was measured. Also, the ecotoxicological effects of dietary exposure to sublethal concentration (1/20 LC50) of these pollutants for 2-week exposure and 1-week recovery on some physiological endpoints evaluated as feeding activity, growth response, and carbonic anhydrase activity as a marker in charge of shell formation and seromucoid level as a marker in charge of mucus synthesis of the snails were studied. The results exhibited that the 48-h LC50 values were 0.91, 313.8, and 45.7 μg/g dry food for ABM, TMX, and ACR, respectively. The sublethal concentrations of these pollutants in the diet after 2-week exposure were found to reduce the food consumption and inhibit growth rate of the snails. Also, the data illustrated that carbonic anhydrase activity was significantly decreased. On the other hand, there was a significant increase in the seromucoid level as a marker responsible for mucus synthesis in ABM- and TMX-exposed snails, while ACR showed significantly decreased level when compared to control. After 1-week recovery, the tested endpoints of treated snails were slightly repaired but still less than that of the untreated animals. The overall outcome of this investigation suggests the utility of this animal as a good bioindicator organism for ABM, TMX, and ACR exposure in pollution monitoring studies.

Keywords

Abamectin Feeding activity Growth response Mucus synthesis Physiological endpoints Shell formation 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

References

  1. Bai S, Ogbourne S (2016) Eco-toxicology effects of the avermectin family with a focus on abamectin and ivermectin. Chemosphere 154:204–214Google Scholar
  2. Barker GM (2001) The Biology of Terrestrial Molluscs. CAB International, Oxon, WallingfordGoogle Scholar
  3. Barman TE (1974) Enzyme Hand book, vol 1. Spering Velag, BerlinGoogle Scholar
  4. Beeby A, Richmond L, Herpe F (2002) Lead reduces shell mass in juvenile garden snails (Helix aspersa). Environ Pollut 120:283–288Google Scholar
  5. Botias C, David AH, Elizabeth M, Goulson D (2016) Contamination of wild plants near neonicotinoid seed-treated crops, and implications for non-target insects. Sci Total Environ 566:269–278Google Scholar
  6. Çoban TA, Beydemir S, Gülçin I, Ekinci D (2007) Morphine inhibits erythrocyte carbonic anhydrase in vitro and in vivo. Biol Pharm Bull 30:2257–2261Google Scholar
  7. CoStat program (2002) Microcomputer program analysis. CoHort software, Version 2.6, MontereyGoogle Scholar
  8. Crowe TP, Smith EL, Donkin P, Barnaby DL, Rowland SJ (2004) Measurements of sub lethal effects on individual organisms indicates community-level impacts of pollution. J Appl Ecol 4:114–123Google Scholar
  9. Denny MW (1983) Molecular biomechanics of mollucan mucous secretions. In: Mollusca WK, Simkiss K, Hochka PW (eds) , vol 1. Academic Press, NY, pp 341–465Google Scholar
  10. El-Gendy KS, Radwan MA, Gad AF (2011) Feeding and growth responses of the snail Theba pisana to dietary metal exposure. Arch Environ Contam Toxicol 60:272–280Google Scholar
  11. Erzen NK, Kolar L, Flajs VC, Kuzner J, Marc I, Pogacnik M (2005) Degradation of abamectin and doramectin on sheep grazed pasture. Ecotoxicology 14:627–635Google Scholar
  12. Finney DJ (1971) Probit analysis, 3rd edn. Cambridge university press, London 318 ppGoogle Scholar
  13. Friedman M (2003) Chemistry, biochemistry, and safety of acrylamide. A review. J Agric Food Chem 51:4504–4526Google Scholar
  14. Gomot-de Vaufleury A, Bispo A (2000) Methods for toxicity assessment of contaminated soil by oral or dermal uptake in land snails. 1. Sublethal effects on growth. Environ Sci Technol 34:1865–1870Google Scholar
  15. Goulson D (2013) An overview of the environmental risks posed by neonicotinoid insecticides. J Appl Ecol 50:977–987Google Scholar
  16. Hasheesh WS, Mohamed RT (2011) Bioassay of two pesticides on Bulinus truncatus snails with emphasis on some biological and histological parameters. Pestic Biochem Physiol 100:1–6Google Scholar
  17. Jansson RK, Dybas RA (1998) Avermectins: Biochemical mode of action, biological activity and agricultural importance. In: Ishaaya I, Degheele D (eds) Insecticides with Novel Modes of Action: Mechanisms and Application. Springer-Verlag, New York, pp 152–167Google Scholar
  18. Kamble SB, Kamble NA (2014) Behavioural changes in freshwater snail Bellamya bengalensis due to acute toxicity of copper sulphate and Acacia sinuate. Int J Sci Environ Technol 3:1090–1104Google Scholar
  19. Kaya ED, Söyüt H, Beydemir S (2013) Carbonic anhydrase activity from the gilthead sea bream (Sparus aurata) liver: the toxicological effects of heavy metals. Environ Toxicol Pharmacol 36:514–521Google Scholar
  20. Kolar L, Jemec A, van Gestel CAM, Valant J, Hrzenjak R, Erzen NK, Zidar P (2010) Toxicity of abamectin to the terrestrial isopod Porcellio scaber (Isopoda, Crustacea). Ecotoxicology 19:917–927Google Scholar
  21. Krishna G, Muralidhara (2015) Inulin supplementation during gestation mitigates acrylamide-induced maternal and fetal brain oxidative dysfunctions and neurotoxicity in rats. Neurotoxicol Teratol 49:49–58Google Scholar
  22. Larguinho M, Cordeiro A, Diniz MS, Costa PM, Baptista PV (2014) Metabolic and histopathological alterations in the marine bivalve Mytilus galloprovincialis induced by chronic exposure to acrylamide. Environ Res 135:55–62Google Scholar
  23. Lionetto MG, Caricato R, Giordano ME, Schettino T (2016) Carbonic anhydrase based biomarkers: potential application in human health and environmental sciences. Curr Biomarkers 6:40–46Google Scholar
  24. Lowry OH, Rasebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275Google Scholar
  25. Ma J, Li XY (2011) Acute toxicity of lambda-cyhalothrin, imidaclopid and avermectin on Physa acuta. J Hydrol 32:100–104Google Scholar
  26. Maioli MA, de Medeiros HCD, Guelfi M, Trinca V, Pereira FTV, Mingatto FE (2013) The role of mitochondria and biotransformation in abamectin-induced cytotoxicity in isolated rat hepatocytes. Toxicol in Vitro 27:570–579Google Scholar
  27. Marin F, Le Roy N, Marie B (2012) The formation and mineralization of mollusk shell. Front Biosci S4:1099–1125Google Scholar
  28. Minakshi R, Mahajan AY (2013) Effect of thiamethoxam on oxygen consumption of the freshwater bivalve, Lamellidens marginalis (Lamarck). The bioscan 8:469–472Google Scholar
  29. Mleiki A, Irizar A, Zaldibar B, El Menif NT, Marigómez I (2016) Bioaccumulation and tissue distribution of Pb and Cd and growth effects in the green garden snail, Cantareus apertus (Born, 1778), after dietary exposure to the metals alone and in combination. Sci Total Environ 547:148–156Google Scholar
  30. Mohamed AM, Bakry FA, Heiba FN (2000) Effect of sublethal concentrations of Abamectin on Biomphalaria alexandrina snails and the free living stages of Schistosoma mansoni. Egyptian J Fish Biol 4:1–15Google Scholar
  31. Pawlicki JM, Pease LB, Pierce CM, Startz TP, Zhang Y, Smith AM (2004) The effect of molluscan glue proteins on gel mechanics. J Exp Biol 207:1127–1135Google Scholar
  32. Pisa LW, Amaral-Rogers V, Belzunces LP, Bonmatin JM, Downs CA, Goulson D, Kreutzweiser DP, Krupke C, Liess M, McField M, Morrissey CA, Noome DA, Settele J, Simon-Delso N, Stark JD, Van der Sluijs JP, Van Dyck H, Wiemers M (2015) Effects of neonicotinoids and fipronil on non-target invertebrates. Environ Sci Pollut Res 22:68–102Google Scholar
  33. Pryce JD (1967) Simplified microestimation of fibrinogen and seromucoid in plasma. Clin Chem 13:650–657Google Scholar
  34. Radwan MA (2016) Comparative toxic effects of some pesticides with different modes of action against the land snail, Theba pisana. Int J Zool Invest 2:170–176Google Scholar
  35. Radwan MA, El-Gendy KS, Gad AF (2010) Biomarkers of oxidative stress in the land snail, Theba pisana for assessing ecotoxicological effects of urban metal pollution. Chemosphere 79:40–46Google Scholar
  36. Ramakrishnan N (2003) Bio-monitoring approaches for water quality assessment in two waterbodies at Tiruvannamalai, Tamil Nadu India. In: Proceedings of the 3rd Int Conf Environ Health, Chennai, India, 15-17 December, pp 374 – 385.Google Scholar
  37. Regoli F, Gorbi S, Fattorini D, Tedesco S, Notti A, Machella N, Bocchetti R, Benedetti M, Piva F (2006) Use of the land snail Helix aspersa as sentinel organism for monitoring ecotoxicologic effects of urban pollution: an integrated approach. Environ Health Perspect 114:63–69Google Scholar
  38. Rodríguez-Eugenio N, McLaughlin M, Pennock D (2018) Soil Pollution: a hidden reality. FAO, Rome pp 142Google Scholar
  39. Santini O, Chahbane N, Vasseur P, Frank H (2011) Effects of low-level copper exposure on Ca2+-ATPase and carbonic anhydrase in the freshwater bivalve Anodonta anatine. Toxicol Environ Chem 93:1826–1837Google Scholar
  40. Schuytema GS, Nebeker AV, Griffis WL (1994) Effects of dietary exposure to forest pesticides on the brown garden snail Helix aspersa Müller. Arch Environ Contam Toxicol 26:23–28Google Scholar
  41. Scott DM, Major CW (1972) The effect of copper (II) on survival, respiration, and heart rate in the common blue mussel, Mytilus edulis. Biol Bull 143:679–688Google Scholar
  42. Snyman RG, Reinecke AJ, Reinecke SA (2002) Field application of a lysosomal assay as biomarker of copper oxychloride exposure, in the snail Helix aspersa. Bull Environ Contam Toxicol 69:117–122Google Scholar
  43. Subaraja M, Vanisree AJ (2015) Cerebral ganglionic variations and movement behaviors of Lumbricus terrestris on exposure to neurotoxin. Ann Neurosci 22:199–207Google Scholar
  44. Sun Y, Diao X, Zhang Q, Shen J (2005) Bioaccumulation and elimination of avermectin B1a in the earthworms (Eisenia foetida). Chemosphere 60:699–704Google Scholar
  45. Swaileh KM, Ezzughayyar A (2000) Effects of dietary Cd and Cu on feeding and growth rates of the land snail Helix engaddensis. Ecotoxicol Environ Saf 47:253–260Google Scholar
  46. Swaileh KM, Ezzughayyar A (2001) Dose-dependent effects of dietary Pb and Zn on feeding and growth rates of the land snail, Helix engaddensis. Ecotoxicol Environ Saf 50:9–14Google Scholar
  47. Tomizawa M, Casida JE (2003) Selective toxicity of neonicotinoids attributable to specificity of insect and mammalian nicotinic receptors. Annu Rev Entomol 48:339–364Google Scholar
  48. Triebskom R, Ebert D (1989) The importance of mucus production in slug's reaction to molluscicides and the impact of molluscicides on the mucus producing system. In: Henderson IF (ed) Slugs and Snails in World Agriculture. BCPC Monograph 41, Thornton Heath, pp 373–379Google Scholar
  49. Triebskorn R, Henderson IF, Martin A, Kohler H-R (1996) Slugs as targets or non-target organisms for environmental chemicals. In: Henderson IF (ed) Slug and Snail Pests in Agriculture. BCPC Monograph 66, Farnharm, pp 65–72Google Scholar
  50. Wilbur KM, Saleuddin ASM (1983) Shell formation. In: Saleuddin ASM, Wilbur KM (eds) The Mollusca, vol 4. Academic Press, New York, pp 235–287Google Scholar
  51. Wojtaszek J, Poloczek-Adamowicz A, Adamowicz A, Fuks U, Dzugaj A (1998) Cytomorphometry and seromucoid concentration in the hemolymph of selected snail species. Zoologica Poloniae 43:87–101Google Scholar
  52. Wood TJ, Goulson D (2017) The environmental risks of neonicotinoid pesticides: a review of the evidence post 2013. Environ Sci Pollut Res 24:17285–17325Google Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Kawther S. El-Gendy
    • 1
    Email author
  • Mohamed A. Radwan
    • 1
  • Amira F. Gad
    • 2
  • Awatef E. Khamis
    • 1
  • El-Sayed H. Eshra
    • 2
  1. 1.Department of Pesticide Chemistry and Technology, Faculty of Agriculture (El-Shatby)University of AlexandriaAlexandriaEgypt
  2. 2.Agriculture Research CenterPlant Protection Research InstituteAlexandriaEgypt

Personalised recommendations