Environmental Science and Pollution Research

, Volume 26, Issue 9, pp 8875–8884 | Cite as

Antioxidant properties and instrumental quality characteristics of spray dried Pangasius visceral protein hydrolysate prepared by chemical and enzymatic methods

  • Mohammad Aman Hassan
  • Martin Xavier
  • Subodh Gupta
  • Binaya Bhusan Nayak
  • Amjad Khansaheb BalangeEmail author
Research Article


Fish protein hydrolysates are digested form of protein with various bioactive properties where, the cleavages of molecular bonds of proteins can be broken by the enzymatic and chemical process. In this study, antioxidant properties of spray dried protein hydrolysate prepared from Pangasius viscera by using enzymatic (papain and pepsin), and chemical methods (hydrochloric acid and sodium hydroxide) were evaluated. Among the different treatments, pepsin-derived visceral protein hydrolysate showed the maximum antioxidant activity when used at higher concentrations. Essential amino acids (EAA) and hydrophobic amino acids are higher in papain-derived visceral protein hydrolysate. In pepsin-derived visceral protein hydrolysate, major proportion was contributed by glycine (Gly), glutamine (Glu), proline (Pro), and asparagine (Asp). Higher amount of aromatic amino acids are found in alkali-derived FVPH. Scanning electron microscopy (SEM) images of pepsin fish visceral protein hydrolysate showed better globular structure than the other treatments. It can be concluded that among the different treatments, the visceral protein hydrolysate prepared with pepsin had better overall quality regarding antioxidant properties and papain in nutritional point of view.


Pangasius viscera Antioxidant activity Amino acid FTIR SEM 



The authors thank the Director, ICAR-Central Institute of Fisheries Education, Mumbai. The Maulana Azad National Fellowship given by UGC, India, to the first author is gratefully acknowledged.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Mohammad Aman Hassan
    • 1
  • Martin Xavier
    • 1
  • Subodh Gupta
    • 2
  • Binaya Bhusan Nayak
    • 1
  • Amjad Khansaheb Balange
    • 1
    Email author
  1. 1.Department of Post-Harvest Technology, FRHPHM DivisionICAR-Central Institute of Fisheries EducationMumbaiIndia
  2. 2.Fish Nutrition, Biochemistry and Physiology DivisionICAR-Central Institute of Fisheries EducationMumbaiIndia

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