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Water pollution and observation of acquired antibiotic resistance in Bayou Lafourche, a major drinking water source in Southeast Louisiana, USA

  • Kyle Bird
  • Raj Boopathy
  • Rajkumar Nathaniel
  • Gary LaFleur
Appropriate Technologies to Combat Water Pollution

Abstract

Antibiotics are known to enter the environment, not only by human excretion but also through livestock/aquaculture, healthcare facilities, and pharmaceutical industry waste. Once in the environment, antibiotics have the ability to provide a selective pressure in microbial communities thus selecting for resistance. Bayou Lafourche of Southeastern Louisiana serves as the raw source of drinking water for 300,000 people in the region and has previously been shown to receive high amounts of fecal contamination. Four sites along the bayou and one site from its input source on the Mississippi River were monitored for water chemistry, total and fecal coliform estimates, and presence of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARG) for a period of 1 year. Four waste-associated bacterial isolates were tested for resistance to antibiotics (tetracycline, sulfamethoxazole/trimethoprim, cefoxitin, meropenem, imipenem, erythromycin, and vancomycin). Resistant bacteria were further examined with PCR/electrophoresis to confirm the presence of antibiotic resistance genes (Sul1, tet(A), tet(W), tet(X), IMP, KPC, and OXA-48). The bayou appears to meet the Louisiana Department of Environmental Quality (LDEQ) criteria for water chemistry, yet fecal coliforms were consistently higher than LDEQ thresholds, thus indicating fecal contamination. Enterobacteriaceae isolates showed 13.6%, 10.9%, and 19.8% resistant to tetracycline, sulfamethoxazole/trimethoprim, and cefoxitin, respectively, and 11 isolates were confirmed for presence of either tet(A) or Sul1 resistance genes. High fecal coliforms and presence of ARB/ARG may both indicate a presence of anthropogenic or agricultural source of fecal contamination.

Keywords

Antibiotics Antibiotic-resistant bacteria Antibiotic resistance genes Tetracycline Sulfonamide Fecal coliform Total coliform 

Notes

Funding

This work was supported by the funds from Louisiana Board of Regents through EPSCoR P-Fund.

References

  1. Abraham EP, Newton GGF (1961) The structure of cephalosporin C. Biochem J 79:377–393CrossRefGoogle Scholar
  2. Aminov RI, Garrigues-Jeanjean N, Mackie RI (2001) Molecular ecology of tetracycline resistance: development and validation of primers for detection of tetracycline resistance genes encoding ribosomal protection proteins. Appl Environ Microbiol 67:22–32CrossRefGoogle Scholar
  3. APHA (1998) Standard methods for analysis of water and wastewater, 20th edn. American Public Health Association, AlexandriaGoogle Scholar
  4. Barr V, Barr K, Millar MR, Lacey RW (1986) β-Lactam antibiotics increase the frequency of plasmid transfer in Staphylococcus aureus. J Antimicrob Chemother 17:409–413CrossRefGoogle Scholar
  5. Bergeron S, Boopathy R, Nathaniel R, Corbin A, LaFleur G (2017) Presence of antibiotic resistance genes in raw source water of a drinking water treatment plant in a rural community of USA. Int Biodeterior Biodegrad 124:3–9CrossRefGoogle Scholar
  6. Blázquez J, Couce A, Rodríguez-Beltrán J, Rodríguez-Rojas A (2012) Antimicrobials as promoters of genetic variation. Curr Opin Microbiol 15:561–569CrossRefGoogle Scholar
  7. Byrne-Bailey KG, Gaze WH, Kay P, Boxall ABA, Hawkey PM, Wellington EMH (2009) Prevalence of sulfonamide resistance genes in bacterial isolates from manured agricultural soils and pig slurry in the United Kingdom. Antimicrob Agents Chemother 53:696–702CrossRefGoogle Scholar
  8. Cabello FC, Godfrey HP, Tomova A, Ivanova L, Dölz H, Millanao A, Buschmann AH (2013) Antimicrobial use in aquaculture re-examined: its relevance to antimicrobial resistance and to animal and human health. Environ Microbiol 15:1917–1942CrossRefGoogle Scholar
  9. Centers for Disease Control and Prevention (CDC) (2013) Antibiotic resistance threats in the United States. Atlanta. http://CDC.gov. Accessed 05 July 2013
  10. Chopra I, Hawkey PM, Hinton M (1992) Tetracy-clines, molecular and clinical aspects. J Antimicrob Chemother 29:245–277CrossRefGoogle Scholar
  11. Cusumano CK, Pinkner JS, Han Z, Greene SE, Ford BA, Crowley JR, Henderson JP, Janetka JW, Hultgren SJ (2011) Treatment and prevention of urinary tract infection with orally active FimH inhibitors. Sci Transl Med 3:109ra115-109ra115CrossRefGoogle Scholar
  12. Delost MD (2014) Introduction to diagnostic microbiology for the laboratory sciences. Jones & Bartlett Publishers, BurlingtonGoogle Scholar
  13. Eliopoulos GM, Huovinen P (2001) Resistance to trimethoprim-sulfamethoxazole. Clin Infect Dis 32:1608–1614CrossRefGoogle Scholar
  14. Everage TJ, Boopathy R, Nathaniel R, LaFleur G, Doucet J (2014) A survey of antibiotic-resistant bacteria in a sewage treatment plant in Thibodaux, Louisiana, USA. Int Biodeterior Biodegrad 95:2–10CrossRefGoogle Scholar
  15. FDA (2015) Summary report on antimicrobials sold or distributed for use in food-producing animals. Available at: https://docs.google.com/viewer?url=http%3A%2F%2Fwww.fda.gov%2Fdownloads%2FForIndustry%2FUserFees%2F. Accessed 22 May 2015
  16. Fick J, Soderstrom H, Lindberg RH, Phan C, Tysklind M, Larsson DGJ (2009) Contamination of surface, ground, and drinking water from pharmaceutical production. Environ Toxicol Chem 28:2522–2527CrossRefGoogle Scholar
  17. Fleming-Dutra KE, Mangione-Smith R, Hicks LA (2016) How to prescribe fewer unnecessary antibiotics: talking points that work with patients and their families. Am Fam Physician 94(3):202–204Google Scholar
  18. Gillings MR, Stokes HW (2012) Are humans increasing bacterial evolvability? Trends Ecol Evol 27:346–352CrossRefGoogle Scholar
  19. Hach (1999) Hach DR/2000 spectrophotometer handbook and manual. HACH Company, Loveland, ColoradoGoogle Scholar
  20. Halling-Sørensen B (2001) Inhibition of aerobic growth and nitrification of bacteria in sewage sludge by antibacterial agents. Arch Environ Contam Toxicol 40:451–460CrossRefGoogle Scholar
  21. Hollis A, Ahmed Z (2013) Preserving antibiotics, rationally. N Engl J Med 369:2474–2476CrossRefGoogle Scholar
  22. Iversen A, Kühn I, Rahman M, Franklin A, Burman LG, Olsson- Liljequist B, Torell E, Möllby R (2004) Evidence for transmission between humans and the environment of a nosocomial strain of Enterococcus faecium. Environ Microbiol 6:55–59CrossRefGoogle Scholar
  23. Jayachandran S, Lleras-Muney A, Smith KV (2010) Modern medicine and the twentieth century decline in mortality: evidence on the impact of sulfa drugs. Am Econ J Appl Econ 2:118–146CrossRefGoogle Scholar
  24. Kalff J (2002) Limnology; inland water ecosystems. Prentice Hall, New JerseyGoogle Scholar
  25. Kay P, Blackwell PA, Boxall AB (2005) Column studies to investigate the fate of veterinary antibiotics in clay soils following slurry application to agricultural land. Chemosphere 60:497–507CrossRefGoogle Scholar
  26. Knapp CW, Dolfing J, Ehlert PA, Graham DW (2009) Evidence of increasing antibiotic resistance gene abundances in archived soils since 1940. Environ Sci Technol 44:580–587CrossRefGoogle Scholar
  27. Kong KF, Schneper L, Mathee K (2010) Beta-lactam antibiotics: from antibiosis to resistance and bacteriology. Apmis 118:1–36CrossRefGoogle Scholar
  28. Levy SB (1992) Active efflux mechanisms for antimicrobial resistance. Antimicrob Agents Chemother 36:695–703CrossRefGoogle Scholar
  29. Louisiana Department of Environmental Quality (2006) Barataria basin 2005 land use/land cover [land use map]. Louisiana Department of Environmental Quality, Baton Rouge Map No. 200601001Google Scholar
  30. Louisiana Department of Environmental Quality 2018a Date accessed: 2018. “Bayou Lafourche Watershed implementation plan: Upper Bayou Lafourche Watershed subsegment 020401.” http://nonpoint.deq.louisiana.gov/wqa/links/watershedplan/Barataria/Bayou%20Lafourche_Final.pdf
  31. Louisiana Department of environmental Quality (2018b) Title 33: environmental quality; Part IX: water quality. http://deq.louisiana.gov/assets/docs/Water/33v09-201605WaterQuality.pdf. Accessed 07 MAy 2018
  32. Naquin A, Shrestha A, Sherpa M, Nathaniel R, Boopathy R (2015) Presence of antibiotic resistance genes in a sewage treatment plant in Thibodaux, Louisiana, USA. Bioresour Technol 188:79–83CrossRefGoogle Scholar
  33. Naquin T, Robichaux K, Belding C, Bergeron S, Boopathy R (2017) Presence of sulfonamide and carbapenem resistance genes in a sewage treatment plant in Southeast Louisiana, USA. Int Biodeterior Biodegrad 124:10–16CrossRefGoogle Scholar
  34. Prabhu DIG, Pandian RS, Vasan PT (2007) Pathogenicity, antibiotic susceptibility and genetic similarity of environmental and clinical isolates of Vibrio cholerae. Indian J Exp Biol 45:817–823Google Scholar
  35. Roberts MC (2005) Update on acquired tetracycline resistance genes. FEMS Microbiol Lett 245:195–203CrossRefGoogle Scholar
  36. Rodríguez C, Lang L, Wang A, Altendorf K, García F, Lipski A (2006) Lettuce for human consumption collected in Costa Rica contains complex communities of culturable oxytetracycline-and gentamicin-resistant bacteria. Appl Environ Microbiol 72:5870–5876CrossRefGoogle Scholar
  37. Sarmah AK, Meyer MT, Boxall AB (2006) A global perspective on the use, sales, exposure pathways, occurrence, fate and effects of veterinary antibiotics (VAs) in the environment. Chemosphere 65(5):725–759CrossRefGoogle Scholar
  38. Schaeffer AJ (2003) The expanding role of fluoroquinolones. Dis Mon 49(2):129–147CrossRefGoogle Scholar
  39. Suarez G, Nathans D (1965) Inhibition of aminoacyl tRNA binding to ribosomes by tetracycline. Biochem Biophys Res Commun 18:743–750CrossRefGoogle Scholar
  40. Suda KJ, Hicks LA, Roberts RM, Hunkler RJ, Taylor TH (2014) Trends and seasonal variation in outpatient antibiotic prescription rates in the United States, 2006 to 2010. Antimicrob Agents Chemother 58:2763–2766CrossRefGoogle Scholar
  41. Sun L, Klein EY, Laxminarayan R (2012) Seasonality and temporal correlation between community antibiotic use and resistance in the United States. Clin Infect Dis 55:687–694CrossRefGoogle Scholar
  42. Thompson SA, Maani EV, Lindell AH, King CJ, McArthur JV (2007) Novel tetracycline resistance determinant isolated from an environmental strain of Serratia marcescens. Appl Environ Microbiol 73:2199–2206CrossRefGoogle Scholar
  43. Vila J, Marti S, Sanchez-Cespedes J (2007) Porins, efflux pumps and multidrug resistance in Acinetobacter baumannii. J Antimicrob Chemother 59:1210–1215CrossRefGoogle Scholar
  44. Watts CD, Crathorne B, Fielding M, Killops SD (1982) Non-volatile organic-compounds in treated waters. Environ Health Perspect 46:87–99CrossRefGoogle Scholar
  45. Zhang XX, Zhang T, Fang HH (2009) Antibiotic resistance genes in water environment. Appl Microbiol Biotechnol 82:397–414CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Kyle Bird
    • 1
  • Raj Boopathy
    • 1
  • Rajkumar Nathaniel
    • 1
  • Gary LaFleur
    • 1
  1. 1.Department of Biological SciencesNicholls State UniversityThibodauxUSA

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