Environmental Science and Pollution Research

, Volume 26, Issue 4, pp 4070–4077 | Cite as

Effects of biochar and dicyandiamide combination on nitrous oxide emissions from Camellia oleifera field soil

  • Bang-Liang Deng
  • Shu-Li Wang
  • Xin-Tong Xu
  • Hua Wang
  • Dong-Nan Hu
  • Xiao-Min Guo
  • Qing-Hua Shi
  • Evan Siemann
  • Ling ZhangEmail author
Research Article


Greenhouse gas emissions from agricultural soils contribute substantially to global atmospheric composition. Nitrous oxide (N2O) is one important greenhouse gas induces global warming. Nitrification inhibitors (NI) or biochar can be effective soil N2O emission mitigation strategies for agricultural soils. However, due to differences in crop physiological traits or agricultural management, the effectiveness of mitigation strategies varies among agricultural systems. Camellia oleifera is a woody oil plant widely grown and requires intensive N input, which will potentially increase N2O emissions. Thereby, mitigation of N2O emissions from C. oleifera field soil is vital for sustainable C. oleifera development. Besides NI, incorporation of C. oleifera fruit shell-derived biochar into its soil will benefit waste management and simultaneous mitigation of N2O emissions but this has not been investigated. Here, we conducted two studies to examine effects of biochar addition and NI (dicyandiamide, DCD) application on N2O emissions from C. oleifera field soil with different N (urea or NH4NO3) and incubation temperatures. Biochar effects on nitrification rates varied among N treatments. Biochar applied in combination with DCD further reduced nitrification rates (for urea treatment, decreased from 1.1 to 0.3 mg kg−1 day−1). Biochar addition consistently increased soil N2O emissions (for urea treatment, increased from 0.03 to 0.08 ng g−1 h−1) and their temperature sensitivity. DCD application reduced soil N2O emissions with greater reductions with urea application. In future cultivation of intensively managed C. oleifera gardens, NI should be applied to mitigate N2O emissions if biochar is added, especially when urea is used.


Biochar application Nitrous oxide Nitrification inhibitors Nitrogen mineralization Camellia oleifera Nitrification Warming 


Funding information

This work was financially supported by the National Natural Science Foundation of China (41501317), National Science and Technology Research Projects (2012BAD14B14-4), China and Jiangxi Postdoctoral Science Foundation (2017M612153, 2017KY18), and Key Science and Technology Project of Jiangxi Education Department (GJJ160348).

Supplementary material

11356_2018_3900_MOESM1_ESM.docx (538 kb)
ESM 1 (DOCX 538 kb)


  1. Bouwman AF (1990) Soils and the greenhouse effect. John Wiley, New YorkGoogle Scholar
  2. Bremner JM (1997) Sources of nitrous oxide in soils. Nutr Cycl Agroecosyst 49:7–16CrossRefGoogle Scholar
  3. Case SDC, McNamara NP, Reay DS, Stott AW, Grant HK, Whitaker J (2015) Biochar suppresses N2O emissions while maintaining N availability in a sandy loam soil. Soil Biol Biochem 81:178–185CrossRefGoogle Scholar
  4. Cheng Y, Wang J, Zhang JB, Müller C, Wang SQ (2015) Mechanistic insights into the effects of N fertilizer application on N2O-emission pathways in acidic soil of a tea plantation. Plant Soil 389:45–57CrossRefGoogle Scholar
  5. Daum D, Schenk MK (1998) Influence of nutrient solution pH on N2O and N2 emissions from a soilless culture system. Plant Soil 203:279–288CrossRefGoogle Scholar
  6. Deng BL, Li ZZ, Zhang L, Ma YC, Li Z, Zhang WY, Guo XM, Niu DK, Siemann E (2016) Increases in soil CO2 and N2O emissions with warming depend on plant species in restored alpine meadows of Wugong Mountain, China. J Soils Sediments 16:777–784CrossRefGoogle Scholar
  7. Fan C, Chen H, Li B, Xiong Z (2017) Biochar reduces yield-scaled emissions of reactive nitrogen gases from vegetable soils across China. Biogeosciences 14:2851–2863CrossRefGoogle Scholar
  8. Fan C, Li B, Xiong Z (2018) Nitrification inhibitors mitigated reactive gaseous nitrogen intensity in intensive vegetable soils from China. Sci Total Environ 612:480–489CrossRefGoogle Scholar
  9. Firestone MK, Davidson EA (1989) Microbiological basis of NO and N2O production and consumption in soil. Exchange of Trace Gases Between Terrestrial Ecosystems and the. Atmosphere 47:7–21Google Scholar
  10. He JZ, Shen JP, Zhang LM, Zhu YG, Zheng YM, Xu MG, Di HJ (2007) Quantitative analyses of the abundance and composition of ammonia-oxidizing bacteria and ammonia-oxidizing archaea of a Chinese upland red soil under long-term fertilization practices. Environ Microbiol 9:2364–2374CrossRefGoogle Scholar
  11. He TH, Liu DY, Yuan JJ, Luo JF, Lindsey S, Bolan N, Ding WX (2018) Effects of application of inhibitors and biochar to fertilizer on gaseous nitrogen emissions from an intensively managed wheat field. Sci Total Environ 628-629:121–130CrossRefGoogle Scholar
  12. Hu HW, Chen DL, He JZ (2015) Microbial regulation of terrestrial nitrous oxide formation: understanding the biological pathways for prediction of emission rates. FEMS Microbiol Rev 39:729–749CrossRefGoogle Scholar
  13. Hu Y, Zhang L, Deng B, Liu Y, Liu Q, Zheng X, Zheng L, Kong F, Guo X, Siemann E (2017) The non-additive effects of temperature and nitrogen deposition on CO2 emissions, nitrification, and nitrogen mineralization in soils mixed with termite nests. Catena 154:12–20CrossRefGoogle Scholar
  14. IPCC (2014) Agriculture, forestry and other land use (AFOLU). In: Climate change 2014: mitigation of climate change. Cambridge University Press, Cambridge, United Kingdom and New York, NY, USA.Google Scholar
  15. Jiang X, Denef K, Stewart CE, Cotrufo MF (2015a) Controls and dynamics of biochar decomposition and soil microbial abundance, composition, and carbon use efficiency during long-term biochar-amended soil incubations. Biol Fertil Soils 52:1–14CrossRefGoogle Scholar
  16. Jiang X, Hou X, Zhou X, Xin X, Wright A, Jia Z (2015b) pH regulates key players of nitrification in paddy soils. Soil Biol Biochem 81:9–16CrossRefGoogle Scholar
  17. Jiang L, Zhang L, Deng B, Liu X, Yi H, Xiang H, Li Z, Zhang W, Guo X, Niu D (2016) Alpine meadow restorations by non-dominant species increased soil nitrogen transformation rates but decreased their sensitivity to warming. J Soils Sediments 17:2329–2337CrossRefGoogle Scholar
  18. Kramer SB, Reganold JP, Glover JD, Bohannan BJ, Mooney HA (2006) Reduced nitrate leaching and enhanced denitrifier activity and efficiency in organically fertilized soils. Proc Natl Acad Sci U S A 103:4522–4527CrossRefGoogle Scholar
  19. Lehmann J, Gaunt J, Rondon M (2006) Biochar sequestration in terrestrial ecosystems–a review. Mitig Adapt Strateg Glob Chang 11:395–419CrossRefGoogle Scholar
  20. Li B, Fan CH, Xiong ZQ, Li QL, Zhang M (2015) The combined effects of nitrification inhibitor and biochar incorporation on yield-scaled N2O emissions from an intensively managed vegetable field in southeastern China. Biogeosciences 12:2003–2017CrossRefGoogle Scholar
  21. Li Z, Zhang L, Deng B, Liu Y, Kong F, Huang G, Zou Q, Liu Q, Guo X, Fu Y, Niu D, Siemann E (2017) Effects of moso bamboo (Phyllostachys edulis) invasions on soil nitrogen cycles depend on invasion stage and warming. Environ Sci Pollut R 24:24989–24999CrossRefGoogle Scholar
  22. Lin Y, Ding W, Liu D, He T, Yoo G, Yuan J, Chen Z, Fan J (2017) Wheat straw-derived biochar amendment stimulated N2O emissions from rice paddy soils by regulating the amoA genes of ammonia-oxidizing bacteria. Soil Biol Biochem 113:89–98CrossRefGoogle Scholar
  23. Liu C, Wang K, Zheng X (2013a) Effects of nitrification inhibitors (DCD and DMPP) on N2O emission, crop yield and nitrogen uptake in a wheat–maize cropping system. Biogeosciences 10:2427–2437CrossRefGoogle Scholar
  24. Liu X, Zhang A, Ji C, Joseph S, Bian R, Li L, Pan G, Paz-Ferreiro J (2013b) Biochar’s effect on crop productivity and the dependence on experimental conditions—a meta-analysis of literature data. Plant Soil 373:583–594CrossRefGoogle Scholar
  25. Liu S, Lin F, Wu S, Cheng J, Sun Y, Jin Y, Li S, Li Z, Zou J (2016) A meta-analysis of fertilizer-induced soil NO and combined with N2O emissions. Glob Chang Biol 23:2520–2532CrossRefGoogle Scholar
  26. Liu J, Wu L, Chen D, Li M, Wei C (2017) Soil quality assessment of different Camellia oleifera stands in mid-subtropical China. Appl Soil Ecol 113:29–35CrossRefGoogle Scholar
  27. Niu Y, Chen Z, Müller C, Zaman MM, Kim D, Yu H, Ding W (2017) Yield-scaled N2O emissions were effectively reduced by biochar amendment of sandy loam soil under maize - wheat rotation in the North China Plain. Atmos Environ 170:58–70CrossRefGoogle Scholar
  28. Shcherbak I, Millar N, Robertson GP (2014) Global meta analysis of the nonlinear response of soil nitrous oxide (N2O) emissions to fertilizer nitrogen. Proc Natl Acad Sci U S A 111:9199–9204CrossRefGoogle Scholar
  29. Shu Q (2013) Cultivation technology for Camellia oleifera. University of Science and Technology of China Press, HefeiGoogle Scholar
  30. Teutscherova N, Lojka B, Houška J, Masaguer A, Benito M, Vazquez E (2018) Application of holm oak biochar alters dynamics of enzymatic and microbial activity in two contrasting Mediterranean soils. Eur J Soil Biol 88:15–26CrossRefGoogle Scholar
  31. Wang J, Chen Z, Xiong Z, Chen C, Xu X, Zhou Q, Kuzyakov Y (2015a) Effects of biochar amendment on greenhouse gas emissions, net ecosystem carbon budget and properties of an acidic soil under intensive vegetable production. Soil Use Manag 31:375–383CrossRefGoogle Scholar
  32. Wang Z, Kong T, Hu S, Sun H, Yang W, Kou Y, Mandlaa XH (2015b) Nitrification inhibitors mitigate earthworm-induced N2O emission—a mesocosm study. Biol Fertil Soils 51:1005–1011CrossRefGoogle Scholar
  33. Wang Q, Hu HW, Shen JP, Du S, Zhang LM, He JZ, Han LL (2017a) Effects of the nitrification inhibitor dicyandiamide (DCD) on N2O emissions and the abundance of nitrifiers and denitrifiers in two contrasting agricultural soils. J Soils Sediments 17:1635–1643CrossRefGoogle Scholar
  34. Wang S, Shan J, Xia Y, Tang Q, Xia L, Lin J, Yan X (2017b) Different effects of biochar and a nitrification inhibitor application on paddy soil denitrification: a field experiment over two consecutive rice-growing seasons. Sci Total Environ 593–594:347–356Google Scholar
  35. Wang J, Zhang B, Tian Y, Zhang H, Cheng Y, Zhang J (2018) A soil management strategy for ameliorating soil acidification and reducing nitrification in tea plantations. Eur J Soil Biol 88:36–40CrossRefGoogle Scholar
  36. Xiao Y, Zhang F, Li Y, Li T, Che Y, Deng S (2018) Influence of winter crop residue and nitrogen form on greenhouse gas emissions from acidic paddy soil. Eur J Soil Biol 85:23–29CrossRefGoogle Scholar
  37. Xie Z, Xu Y, Liu G, Liu Q, Zhu J, Tu C, Amonette J, Cadisch G, Yong JH, Hu S (2013) Impact of biochar application on nitrogen nutrition of rice, greenhouse-gas emissions and soil organic carbon dynamics in two paddy soils of China. Plant Soil 370:527–540CrossRefGoogle Scholar
  38. Yao Z, Wei Y, Liu C, Zheng X, Xie B (2015) Organically fertilized tea plantation stimulates N2O emissions and lowers NO fluxes in subtropical China. Biogeosciences 12:5915–5928CrossRefGoogle Scholar
  39. Zhang A, Liu Y, Pan G, Hussain Q, Li L, Zheng J, Zhang X (2012) Effect of biochar amendment on maize yield and greenhouse gas emissions from a soil organic carbon poor calcareous loamy soil from Central China Plain. Plant Soil 351:263–275CrossRefGoogle Scholar
  40. Zhang M, Fan CH, Li QL, Li B, Zhu YY, Xiong ZQ (2015) A 2-yr field assessment of the effects of chemical and biological nitrification inhibitors on nitrous oxide emissions and nitrogen use efficiency in an intensively managed vegetable cropping system. Agric Ecosyst Environ 201:43–50CrossRefGoogle Scholar
  41. Zhang K, Chen L, Li Y, Brookes PC, Xu J, Luo Y (2017) The effects of combinations of biochar, lime, and organic fertilizer on nitrification and nitrifiers. Biol Fertil Soils 53:77–87CrossRefGoogle Scholar
  42. Zou J, Huang Y, Jiang J, Zheng X, Sass RL (2005) A 3-year field measurement of methane and nitrous oxide emissions from rice paddies in China: effects of water regime, crop residue, and fertilizer application. Global Biogeochem Cy 19:GB2021Google Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Ministry of Education and Jiangxi Key Laboratory of Crop Physiology, Ecology and Genetic BreedingJiangxi Agricultural UniversityNanchangChina
  2. 2.College of Land Resources and EnvironmentJiangxi Agricultural UniversityNanchangChina
  3. 3.Department of BiosciencesRice UniversityHoustonUSA

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