Tritium (3H) is a radioactive isotope of hydrogen. In the environment, the most common form of tritium is tritiated water (HTO). However, tritium can also be incorporated into organic molecules, forming organically bound tritium (OBT). The present study characterized the effects of tritium on the health of the fathead minnow, Pimephales promelas. Fish were exposed to a gradient of HTO (activity concentrations of 12,000, 25,000, and 180,000 Bq/L) and OBT using food spiked with tritiated amino acids (OBT only, with an activity concentration of 27,000 Bq/L). A combined exposure condition where fish were placed in 25,000 Bq/L water and received OBT through feed was also studied. Fish were exposed for 60 days, followed by a 60-day depuration period. A battery of health biomarkers were measured in fish tissues at seven time points throughout the 120 days required to complete the exposure and depuration phases. HTO and OBT were also measured in fish tissues at the same time points. Results showed effects of increasing tritium activity concentrations in water after 60 days of exposure. The internal dose rates of tritium, estimated from the tissue free-water tritium (TFWT) and OBT activity concentrations, reached a maximum of 0.65 μGy/h, which is relatively low considering background levels. No effects were observed on survival, fish condition, and metabolic indices (gonado-, hepato-, and spleno-somatic indexes (GSI, HSI, SSI), RNA/DNA and proteins/DNA ratios). Multivariate analyses showed that several biomarkers (DNA damages, micronucleus frequency, brain acetylcholinesterase, lysosomal membrane integrity, phagocytosis activity, and reactive oxygen species production) were exclusively correlated with fish tritium internal dose rate, showing that tritium induced genotoxicity, as well as neural and immune responses. The results were compared with another study on the same fish species where fish were exposed to tritium and other contaminants in natural environments. Together with the field study, the present work provides useful data to identify biomarkers for tritium exposure and better understand modes of action of tritium on the fathead minnow.
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The authors are grateful for Jerry Piekarski at Lipid Analytical (University of Guelph) for performing the lipid analysis. The authors also want to thank Matt Bond, Jennifer Olfert, and Joanne Ball (CNL) for carefully reviewing the manuscript.
– Following a previously published field investigation, this study aims to characterize tritium effects on fish health under controlled conditions in a laboratory setting.
– Fathead minnows were exposed to tritium activity concentrations up to 180,000 Bq/L.
– At the highest levels of exposure, tritium increased DNA damage and modulated the immune responses.
– Other markers were affected, including the neural system, oxidative stress, and fatty acid composition.
– No effects are reported on the measured health indices and antioxidant activities.
Responsible editor: Philippe Garrigues
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Gagnaire, B., Gosselin, I., Festarini, A. et al. Effects of in vivo exposure to tritium: a multi-biomarker approach using the fathead minnow, Pimephales promelas. Environ Sci Pollut Res 27, 3612–3623 (2020). https://doi.org/10.1007/s11356-018-3781-5
- Fathead minnow, Pimephales promelas
- Tritium internal dose rate
- In vivo exposure
- Immune system response
- Oxidative stress response
- Neural response
- Fatty acid composition