Protective effect of cinnamon against acetaminophen-mediated cellular damage and apoptosis in renal tissue
Acetaminophen, APAP, is a common over-the-counter drug with antipyretic-analgesic action. When APAP is used in large doses, it causes hepatotoxicity and nephrotoxicity but safe at therapeutic doses. Cinnamon (Cinnamomum zeylanicum) is extensively used in folk medicine due to its high content of natural antioxidants. The current investigation was planned to study the possible ameliorative effect of cinnamon toward induced APAP-apoptosis and cellular damage in renal cells. Four groups (nine rats each) were used; negative control group administrated distilled water for 15 days; positive control APAP group administrated a single dose of APAP (1 g/kg) orally on the last day; APAP+Cin L (200 mg/kg) and APAP+Cin H (400 mg/kg) aqueous extract of cinnamon orally once a day for 15 days. An hour after the last dose of cinnamon, all rats in the third and fourth group were administrated a single dose of APAP (1 g/kg) orally. GC/MS analysis was performed to identify the plant used in the study. APAP markedly increased serum levels of creatinine, BUN, and glucose and decreased levels of albumin and total protein. In addition, APAP could also exert severe alteration in the kidney histopathology along with upregulation of caspase-3 and PCNA. However, pre-treatment with cinnamon ameliorated the APAP-induced cellular alterations and apoptosis, possibly through its high content of antioxidants.
KeywordsAcetaminophen Nephrotoxicity Cinnamomum zeylanicum GC/MS Free radical Antioxidant Anti-apoptosis Caspase-3 PCNA
All authors are appreciating the technical support provided by the staff members of the Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University for implementing this study.
Compliance with ethical standards
All the procedures related to animal experimentation were performed according to the guide for the care and use of laboratory animals of the National Institutes of Health (NIH publication No. 85–23, revised 1996) on the protection of animals used for scientific purposes and approved by the ethics committee, Faculty of Veterinary Medicine, Benha University, Egypt.
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