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Environmental Science and Pollution Research

, Volume 26, Issue 1, pp 240–249 | Cite as

Protective effect of cinnamon against acetaminophen-mediated cellular damage and apoptosis in renal tissue

  • Ahmed Abdeen
  • Afaf Abdelkader
  • Mohamed Abdo
  • Gamal Wareth
  • Mohamed Aboubakr
  • Lotfi Aleya
  • Mohamed Abdel-Daim
Research Article

Abstract

Acetaminophen, APAP, is a common over-the-counter drug with antipyretic-analgesic action. When APAP is used in large doses, it causes hepatotoxicity and nephrotoxicity but safe at therapeutic doses. Cinnamon (Cinnamomum zeylanicum) is extensively used in folk medicine due to its high content of natural antioxidants. The current investigation was planned to study the possible ameliorative effect of cinnamon toward induced APAP-apoptosis and cellular damage in renal cells. Four groups (nine rats each) were used; negative control group administrated distilled water for 15 days; positive control APAP group administrated a single dose of APAP (1 g/kg) orally on the last day; APAP+Cin L (200 mg/kg) and APAP+Cin H (400 mg/kg) aqueous extract of cinnamon orally once a day for 15 days. An hour after the last dose of cinnamon, all rats in the third and fourth group were administrated a single dose of APAP (1 g/kg) orally. GC/MS analysis was performed to identify the plant used in the study. APAP markedly increased serum levels of creatinine, BUN, and glucose and decreased levels of albumin and total protein. In addition, APAP could also exert severe alteration in the kidney histopathology along with upregulation of caspase-3 and PCNA. However, pre-treatment with cinnamon ameliorated the APAP-induced cellular alterations and apoptosis, possibly through its high content of antioxidants.

Keywords

Acetaminophen Nephrotoxicity Cinnamomum zeylanicum GC/MS Free radical Antioxidant Anti-apoptosis Caspase-3 PCNA 

Notes

Acknowledgments

All authors are appreciating the technical support provided by the staff members of the Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University for implementing this study.

Compliance with ethical standards

All the procedures related to animal experimentation were performed according to the guide for the care and use of laboratory animals of the National Institutes of Health (NIH publication No. 85–23, revised 1996) on the protection of animals used for scientific purposes and approved by the ethics committee, Faculty of Veterinary Medicine, Benha University, Egypt.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Forensic Medicine and Toxicology, Faculty of Veterinary MedicineBenha UniversityToukhEgypt
  2. 2.Department of Forensic Medicine and Clinical Toxicology, Faculty of MedicineBenha UniversityBenhaEgypt
  3. 3.Department of Anatomy and Embryology, Faculty of Veterinary MedicineUniversity of Sadat CitySadatEgypt
  4. 4.Department of Pathology, Faculty of Veterinary MedicineBenha UniversityToukhEgypt
  5. 5.Department of Pharmacology, Faculty of Veterinary MedicineBenha UniversityToukhEgypt
  6. 6.Chrono-Environment Laboratory, Bourgogne Franche-Comté UniversityUMR CNRS 6249Besançon CedexFrance
  7. 7.Pharmacology Department, Faculty of Veterinary MedicineSuez Canal UniversityIsmailiaEgypt

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