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Environmental Science and Pollution Research

, Volume 25, Issue 36, pp 36117–36123 | Cite as

Cytogenetic and genotoxic effects of 2-chlorophenol on Allium cepa L. root meristem cells

  • Derya Küçük
  • Recep Liman
Research Article
  • 89 Downloads

Abstract

2-Chlorophenol (2-CP), a class of chlorinated organic pollutants like other chlorophenols, is used as intermediate in the synthesis of the higher chlorinated congeners, certain dyes, preservatives, herbicides, fungicides, and plastics. In this study, cytotoxic and genotoxic effects of 2-CP were investigated on the root meristem cells of Allium cepa for its effects on root growth, mitotic index (MI), mitotic phases, chromosomal abnormalities (CAs), and DNA damage by using Allium anaphase-telophase and Comet assays. EC50 of 2-CP value was determined as approximately 25 mg/L by Allium root growth inhibition test. Three concentrations of 2-CP (12.5, 25, and 50 mg/L), distilled water (negative control), and methyl methane sulfonate (MMS, 10 mg/L, positive control) were applied to onion stem cells under different exposure periods (24, 48, 72, and 96 h). All the applied doses of 2-CP slightly decreased MIs. 2-CP induced total CAs such as disturbed anaphase-telophase, chromosome laggards, stickiness, and bridges and also DNA damage at significant levels. These results demonstrate that 2-CP has genotoxic effects in A. cepa root meristematic cells.

Keywords

2-Chlorophenol Allium cepa Genotoxicity Toxicity Comet assay Chromosome aberration 

Notes

Funding information.

This work was financially supported by Usak University BAP (Project no. 2017/TP027).

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Faculty of Arts and Sciences, Molecular Biology and Genetics DepartmentUşak UniversityUşakTurkey

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