Environmental Science and Pollution Research

, Volume 25, Issue 36, pp 36136–36146 | Cite as

Effect of PM2.5 environmental pollution on rat lung

  • Biao Yang
  • Jie Guo
  • Chunling XiaoEmail author
Research Article


Particulate matter smaller than 2.5 μm (PM2.5) is a continuing challenge to pulmonary health. Here, we investigated the mechanisms involved in PM2.5 exposure-induced acute lung injury in rats. We analyzed biochemical and morphological changes following a 2-week “real-world” exposure. And then we found that PM2.5 exposure increased the concentrations of total protein, malondialdehyde, hydrogen peroxide, nitric oxide, and soluble elastin in bronchoalveolar lavage fluid, levels of cytokines in blood, and expression of MMP-9 in airways. Further, alveolar macrophage and neutrophil counts increased following PM2.5 exposure, and edema and lung lesions were observed. Our results suggest that PM2.5 exposure can induce oxidative stress and acute inflammatory responses, which can damage the micro-environment and decrease the repair ability of the lung, resulting in tissue damage.


PM2.5 Lung Injury Inflammation Oxidative stress Extracellular matrix 



particulate matter with an aerodynamic diameter less than 2.5 μm


neutral saline




hydrogen peroxide


bronchoalveolar Lavage (BAL) fluid


alveolar macrophages


hematoxylin and eosin


reactive oxygen species


ethylene diamine tetraacetic acid


phosphate buffer sulfate


bicinchoninic acid


enzyme-linked immunosorbent assay


DNA-binding dye propidium iodide


Evans blue dye


acute lung injury


analysis of variance


cholinergic neurotrophic factor




monocyte chemotactic protein 1




receptor for advanced glycation endproducts


tissue inhibitor of metalloproteinase-1


deoxyribonucleic acid


transmission electron microscopy


Funding information

This study was financially supported by the National Natural Science Foundation of China (30872083) and Shenyang Nonprofit Science and Technology Project.

Compliance with ethical standards

All studies were performed according to protocols reviewed and approved by the Ethics Committee of Animal Care and Experimentation of the National Institute for Environmental Studies, China.

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

11356_2018_3492_MOESM1_ESM.docx (38 kb)
Table S1 (DOCX 38 kb)
11356_2018_3492_MOESM2_ESM.jpg (620 kb)
ESM 1 (JPG 619 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Key Lab of Environmental Pollution and Microecology of Liaoning ProvinceShenyang Medical CollegeShenyangPeople’s Republic of China

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