Heat processing effect of luteolin on anti-metastasis activity of human glioblastoma cells U87
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Among the flavonoïds, luteolin is a flavone that has been identified in many plants. It is known for its apoptotic potential with damage to DNA and cell cycle blockage. Many studies have shown that luteolin has anti-oxidant, anti-inflammatory, and anti-cancer activities. However, it is known that heat treatment (boiling, cooking, and treating with microwaves …) can influence the structure of flavonoïds, which often leads to changes in their activities. The present study was conducted to study the effect of heated luteolin on anti-tumor activity of glioblastoma cells U87. Glioblastoma cell viability was evaluated by MTT assay. Adhesion assay was performed on different protein matrices (collagen type 1, vitronectin, fibronectin, and poly-L-lysine); migration assay was determined by modified Boyden chambers and videomicroscopy, and finally, angiogenesis was tested in vitro by capillary network formation on Matrigel™. The results obtained show that the thermal treatment significantly reduces its cytotoxic activity and ability to inhibit cell adhesion to different protein matrices. It was also found that the heat processed significantly reduced the ability of luteolin to inhibit cell migration, cell invasion, and endothelial cell angiogenesis (HMEC-1). This suggests that heat treated luteolin has a lower anti-tumor potential than native luteolin.
KeywordsHeat processing Glioblastoma Adhesion Migration Invasion Angiogenesis
Thiazolyl blue tetrazolium bromide
Human glioblastoma cells
Human microvascular endothelial cells
Fetal bovine serum
Half maximal inhibitory concentration
Bovine serum albumin
Sodium dodecyl sulfate
Human brain glioblastoma cell
Human lung cancer cells
Human breast cancer cell
Human breast cancer cell
Human prostate cancer cell
Eagle’s minimum essential medium
Dorra El Gueder was responsible for the conception and design, testing and data acquisition, analysis, and data interpretation and drafted the manuscript.
Mouna Maatouk, Zahar Kalboussi, Dhouafi Zaineb, Hind Chaabane, and Irina Ioannou were involved in drafting the manuscript.
Kamel Ghedira, Leila Chekir Ghedira, and Jose Luis have contribution to conception and revised it critically for important intellectual content.
The authors acknowledge the «Ministère Tunisienne de l’Enseignement Supérieur et de la Recherche Scientifique» for the financial support of this study. This research was supported by the Institut National de la Santé et de la Recherche Médicale (INSERM) and by grants from SIRIC (INCa, Institut National du Cancer).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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