Environmental Science and Pollution Research

, Volume 25, Issue 36, pp 36545–36554 | Cite as

Heat processing effect of luteolin on anti-metastasis activity of human glioblastoma cells U87

  • Dorra El GuederEmail author
  • Mouna Maatouk
  • Zahar Kalboussi
  • Zaineb Daouefi
  • Hind Chaaban
  • Irina Ioannou
  • Kamel Ghedira
  • Leila Chekir Ghedira
  • José Luis
Research Article


Among the flavonoïds, luteolin is a flavone that has been identified in many plants. It is known for its apoptotic potential with damage to DNA and cell cycle blockage. Many studies have shown that luteolin has anti-oxidant, anti-inflammatory, and anti-cancer activities. However, it is known that heat treatment (boiling, cooking, and treating with microwaves …) can influence the structure of flavonoïds, which often leads to changes in their activities. The present study was conducted to study the effect of heated luteolin on anti-tumor activity of glioblastoma cells U87. Glioblastoma cell viability was evaluated by MTT assay. Adhesion assay was performed on different protein matrices (collagen type 1, vitronectin, fibronectin, and poly-L-lysine); migration assay was determined by modified Boyden chambers and videomicroscopy, and finally, angiogenesis was tested in vitro by capillary network formation on Matrigel™. The results obtained show that the thermal treatment significantly reduces its cytotoxic activity and ability to inhibit cell adhesion to different protein matrices. It was also found that the heat processed significantly reduced the ability of luteolin to inhibit cell migration, cell invasion, and endothelial cell angiogenesis (HMEC-1). This suggests that heat treated luteolin has a lower anti-tumor potential than native luteolin.

Graphical abstract


Heat processing Glioblastoma Adhesion Migration Invasion Angiogenesis 





Thiazolyl blue tetrazolium bromide


Human glioblastoma cells


Human microvascular endothelial cells


Dimethyl sulfoxide


Fetal bovine serum


Carbon dioxide


Half maximal inhibitory concentration


Extracellular matrix


Bovine serum albumin


Sodium dodecyl sulfate


Human brain glioblastoma cell


Human lung cancer cells


Human breast cancer cell


Human breast cancer cell


Human prostate cancer cell




Heated luteolin


Eagle’s minimum essential medium


Author contributions

Dorra El Gueder was responsible for the conception and design, testing and data acquisition, analysis, and data interpretation and drafted the manuscript.

Mouna Maatouk, Zahar Kalboussi, Dhouafi Zaineb, Hind Chaabane, and Irina Ioannou were involved in drafting the manuscript.

Kamel Ghedira, Leila Chekir Ghedira, and Jose Luis have contribution to conception and revised it critically for important intellectual content.

Funding information

The authors acknowledge the «Ministère Tunisienne de l’Enseignement Supérieur et de la Recherche Scientifique» for the financial support of this study. This research was supported by the Institut National de la Santé et de la Recherche Médicale (INSERM) and by grants from SIRIC (INCa, Institut National du Cancer).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Dorra El Gueder
    • 1
    • 2
    Email author
  • Mouna Maatouk
    • 2
  • Zahar Kalboussi
    • 2
  • Zaineb Daouefi
    • 1
    • 2
  • Hind Chaaban
    • 3
  • Irina Ioannou
    • 3
  • Kamel Ghedira
    • 4
  • Leila Chekir Ghedira
    • 2
  • José Luis
    • 5
  1. 1.Faculty of Sciences of TunisUniversity of Tunis El ManarTunisTunisia
  2. 2.Faculty of Dental Medicine, Unity of Bioactive and Natural Substances and Biotechnology UR17ES49University of MonastirMonastirTunisia
  3. 3.National School of Agronomy and Food Industries, Laboratory of Bimolecular EngineeringNational Polytechnics Institute of Lorraine ENSAIA-INPLNancyFrance
  4. 4.Faculty of Pharmacy, Department of Pharmaceutical SciencesUniversity of MonastirMonastirTunisia
  5. 5.CNRS, Institut de NeurophysiopathologieAix Marseille UniversityMarseilleFrance

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