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Chitosan oligosaccharides alleviate PM2.5-induced lung inflammation in rats

  • Yingzheng Zhao
  • Guangcui Xu
  • Shouying Wang
  • Xianwen Yi
  • Weidong Wu
Research Article
  • 28 Downloads

Abstract

Air pollution of particulate matter (PM), especially PM2.5, has become a major public health problem in China. Exploration of therapeutic and preventive measures against PM2.5 toxicity is of practical significance. The aim of this study was to examine the inhibitory effects of chitosan oligosaccharides (COS) on PM2.5-induced lung inflammation in rats. Forty SPF (specific pathogen-free) male Wistar rats weighing 200–220 g were randomly divided into four groups: control group, COS group, PM2.5 group, and PM2.5+COS group. COS was pre-administered to rats by gavage at a single dose of 500 mg/kg 2 h before intratracheal instillation of PM2.5 at a single dose of 1.2 mg/kg daily for 3 consecutive days. Normal saline (NS) was used as negative control. Twenty-four hours after the last instillation of PM2.5, rats were sacrificed and subjected to bronchoalveolar lavage (BAL). The BAL fluids (BALF) were collected for measurement of levels of total proteins, lactate dehydrogenase (LDH), interleukin-1 (IL-1β), IL-8, and tumor necrosis factor-ɑ (TNF-ɑ) using colorimetric or ELISA kits. Levels of total proteins, LDH activities, and pro-inflammatory mediators including IL-1β, IL-8, and TNF-ɑ in BALF of rats in PM2.5 group significantly increased in comparison with those of the control group. Pre-treatment of rats with COS markedly blocked PM2.5-induced increase in LDH, IL-8, and TNF-ɑ levels in BALF. In conclusion, PM2.5 exposure induces rat lung inflammation, which could be ameliorated by the pre-treatment of COS.

Keywords

Air pollution Particulate matter Lung inflammation Chitosan oligosaccharides 

Abbreviations

PM

particulate matter

NS

normal saline

BALF

bronchoalveolar lavage fluids

LDH

lactate dehydrogenase

IL

interleukin

COS

chitosan oligosaccharides

LD50

the median lethal dose

TNF-ɑ

tumor necrosis factor-ɑ

ROS

reactive oxygen species

iNOS

inducible nitric oxide synthase

GSH

glutathione

SOD

superoxide dismutase

NF-κB

nuclear transcription factor κB

Notes

Author contributions

Weidong Wu designed the study and revised the manuscript. Yingzheng Zhao and Guangcui Xu conducted the experiments. Shouying Wang drafted the manuscript and analyzed the data. Xianwen Yi participated in the design of this project and revised the manuscript. All listed authors have read and approved the final manuscript.

Funding information

This study was funded by the National Natural Science Foundation of China (81573112; 81373030; 81370916), the Henan International Collaborative Laboratory for Air Pollution Health Effects and Intervention, and the Basic and Frontier Technology Research Grant of Henan Province (144300510055).

Compliance with ethical standards

Animal use and care procedures were approved by the Institutional Animal Care and Use Committee of the Xinxiang Medical University strictly in accordance with the Guidelines of Xinxiang Medical University for Animal Experiments.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Yingzheng Zhao
    • 1
  • Guangcui Xu
    • 1
  • Shouying Wang
    • 1
  • Xianwen Yi
    • 1
  • Weidong Wu
    • 1
  1. 1.School of Public HealthXinxiang Medical UniversityXinxiangPeople’s Republic of China

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