Environmental Science and Pollution Research

, Volume 25, Issue 34, pp 34247–34261 | Cite as

Silver and gold nanoparticles biosynthesized by aqueous extract of burdock root, Arctium lappa as antimicrobial agent and catalyst for degradation of pollutants

  • Thi Thanh-Ngan Nguyen
  • Thanh-Truc Vo
  • Bich Ngoc-Huong Nguyen
  • Dinh-Truong Nguyen
  • Van-Su Dang
  • Chi-Hien Dang
  • Thanh-Danh NguyenEmail author
Research Article


This study presents an efficient and facile method for biosynthesis of silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) using aqueous extract of burdock root (BR), A. lappa, and their applications. The nanoparticles were characterized by ultraviolet-visible spectrophotometry, X-ray diffraction, transmission electron microscopy, energy dispersive X-ray, thermogravimetry, and differential thermal analysis. AgNPs capped the BR extract (BR-AgNPs) possessed roughly spherical geometry with an average diameter of 21.3 nm while uneven geometry of AuNPs capped the BR extract (BR-AuNPs) showed multi shapes in average size of 24.7 nm. The BR-AgNPs strongly inhibited five tested microorganism strains. In particular, the nanoparticles showed excellent catalytic activity for the conversion of pollutants within wastewater. Pseudo-first-order rate constants for the degradation of 4-nitrophenol, methyl orange, and rhodamine B were respectively found 6.77 × 10−3, 3.70 × 10−3, and 6.07 × 10−3 s−1 for BR-AgNPs and 6.87 × 10−3, 6.07 × 10−3, and 7.07 × 10−3 s−1 for BR-AuNPs.

Graphical abstract


Nanoparticles Degradation Pollutants Arctium lappa Antimicrobial Catalyst 


Funding information

This project was funded by Vietnam Academy of Science and Technology (VAST) (No. ĐLTE00.04/18-19).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Ahmed S, Ahmad M, Saifullah SBL, Ikram S (2016) Green synthesis of silver nanoparticles using Azadirachta indica aqueous leaf extract. J Radiat Res Appl Sci 9:1–7. CrossRefGoogle Scholar
  2. Ai L, Jiang J (2013) Catalytic reduction of 4-nitrophenol by silver nanoparticles stabilized on environmentally benign macroscopic biopolymer hydrogel. Bioresour Technol 132:374–377. CrossRefGoogle Scholar
  3. Albanese A, Tang PS, Chan WC (2012) The effect of nanoparticles size, shape, and surface chemistry on biological systems. Annu Rev Biomed Eng 14:1–16. CrossRefGoogle Scholar
  4. Arya G, Kumari RM, Gupta N, Kumar A, Channdra R, Nimesh S (2017) Green synthesis of silver nanoparticles using Prosopis juliflora bark extract: reaction optimization, antimicrobial and catalytic activities. Artif Cells Nanomed Biotechnol 18:1–9. CrossRefGoogle Scholar
  5. Balouiri M, Sadiki M, Ibnsouda SK (2016) Methods for in vitro evaluating antimicrobial activity: a review. J Pharm Anal 6:71–79. CrossRefGoogle Scholar
  6. Bello BA, Bello A, Khan SA, Khan JA, Syed FQ, Anwar Y, Khan SB (2017) Antiproliferation and antibacterial effect of biosynthesized AgNPs from leaves extract of Guiera senegalensis and its catalytic reduction on some persistent organic pollutants. J Photochem Photobiol B 175:99–108. CrossRefGoogle Scholar
  7. Chan YS, Cheng LN, Wu JH, Chan E, Kwan YW, Lee SMY, Leung GPH, Yu PHF, Chan SW (2011) A review of the pharmacological effects of Arctium lappa (burdock). Inflammopharmacol 19:245–254. CrossRefGoogle Scholar
  8. Choi Y, Kang S, Cha SH, Kim HS, Song K, Lee YJ, Kim K, Kim YS, Cho S, Park Y (2018) Platycodon saponins from Platycodi radix (Platycodon grandiflorum) for the green synthesis of gold and silver nanoparticles, Nanoscale Res Lett (2018) 13:23
  9. Corbett JF (1972) Pseudo first-order kinetics. J Chem Educ 49:663CrossRefGoogle Scholar
  10. Dutta PP, Bordoloi M, Gogoi K, Roy S, Narzary B, Bhattacharyya DR, Mohapatra PK, Mazumder B (2017) Antimalarial silver and gold nanoparticles: green synthesis, characterization and in vitro study. Biomed Pharmacother 91:567–580. CrossRefGoogle Scholar
  11. Fayaz AM, Balaji K, Girilal M, Yadav R, Kalaichelvan PT, Venketesan R (2010) Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics: a study against gram-positive and gram-negative bacteria. Nanomedicine 6:103–109. CrossRefGoogle Scholar
  12. Francis S, Joseph S, Koshy EP, Mathew B (2017) Synthesis and characterization of multifunctional gold and silver nanoparticles using leaf extract of Naregamia alata and their applications to catalysis and control of mastitis. New J Chem 41:14288–14298. CrossRefGoogle Scholar
  13. Gavade NL, Kadam AN, Suwarnkar MB, Ghodake VP, Garadkar KM (2015) Biogenic synthesis of multi-applicative silver nanoparticles by using Ziziphus jujuba leaf extract. Spectrochim Acta A Mol Biomol Spectrosc 136:953–960. CrossRefGoogle Scholar
  14. Gondwal M, Pant GJN (2018) Synthesis and catalytic and biological activities of silver and copper nanoparticles using Cassia occidentalis. Int J Biomater Article ID 6735426. CrossRefGoogle Scholar
  15. Hadacek F, Greger H (2000) Testing of antifungal natural products: methodologies, comparability of results and assay choice. Phytochem Anal 11:137–147.<137::AID-PCA514>3.0.CO;2-I CrossRefGoogle Scholar
  16. Hernandez-Pinero JL, Terron-Rebolledo M, Foroughbakhch R, Moreno-Limón S, Melendrez MF, Solís-Pomar F, Pérez-Tijerina E (2016) Effect of heating rate and plant species on the size and uniformity of silver nanoparticles synthesized using aromatic plant extracts. Appl Nanosci 6:1183–1190. CrossRefGoogle Scholar
  17. Iravani S (2011) Green synthesis of metal nanoparticles using plants. Green Chem 13:2638–2650. CrossRefGoogle Scholar
  18. Joseph S, Mathew B (2015a) Microwave assisted facile green synthesis of silver and gold nanocatalysts using the leaf extract of Aerva lanata. Spectrochim Acta A Mol Biomol Spectrosc 136:1371–1379. CrossRefGoogle Scholar
  19. Joseph S, Mathew B (2015b) Facile synthesis of silver nanoparticles and their application in dye degradation. Mater Sci Eng B 195:90–97. CrossRefGoogle Scholar
  20. Khan T, Khan MA, Nadhman A (2015) Synthesis in plants and plant extracts of silver nanoparticles with 306 potent antimicrobial properties: current status and future prospects. Appl Microbiol Biotechnol 99:9923–9934. CrossRefGoogle Scholar
  21. Lai TL, Yong KF, Yu JW, Chen JH, Shu YY, Wang CB (2011) High efficiency degradation of 4-nitrophenol by microwave-enhanced catalytic method. J Hazard Mater 185:366–372. CrossRefGoogle Scholar
  22. Mahadevan S, Vijayakumar S, Arulmozhi P (2017) Green synthesis of nanoparticles from Atalantia monophylla (L) Correa leaf extract, their antimicrobial activity and sensing capability of H2O2. Microb Pathog 113:445–450. CrossRefGoogle Scholar
  23. Misha A, Kumari M, Pandey S, Chaudhry V, Gupta K, Nautiyal C (2014) Biocatalytic and antimicrobial activities of gold nanoparticles synthesized by Trichoderma sp. Bioressour technol 166:235–142. CrossRefGoogle Scholar
  24. Morales-Luckie RA, Lopezfuentes-Ruiz AA, Olea-Mejía OF, Liliana A-F, Sanchez-Mendieta V, Brostow W, Hinestroza JP (2016) Synthesis of silver nanoparticles using aqueous extracts of Heterotheca inuloides as reducing agent and natural fibers as templates: Agave lechuguilla and silk. Mater Sci Eng C 69:429–436. CrossRefGoogle Scholar
  25. Nag S, Pramanik A, Chattopadhyay D, Bhattacharyya M (2018) Green-fabrication of gold nanomaterials using Staphylococcus warneri from Sundarbans estuary: an effective recyclable nanocatalyst for degrading nitro aromatic pollutants. Environ Sci Pollut Res 25:2331–2349. CrossRefGoogle Scholar
  26. Naraginti S, Li Y (2017) Preliminary investigation of catalytic, antioxidant, anticancer and bactericidal activity of green synthesized silver and gold nanoparticles using Actinidia deliciosa. J Photochem Photobiol B 170:225–234. CrossRefGoogle Scholar
  27. Naraginti S, Sivakumar A (2014) Eco-friendly synthesis of silver and gold nanoparticles with enhanced bactericidal activity and study of silver catalyzed reduction of 4-nitrophenol. Spectrochim Acta A Mol Biomol Spectrosc 128:357–362. CrossRefGoogle Scholar
  28. Nguyen TD, Dang VS, Nguyen VH, Nguyen TMT, Dang CH (2018a) Synthesis and photophysical characterization of several 2,3-quinoxaline derivatives. An application of Pd(0)/PEG nanoparticle catalyst for Sonogashira coupling. Polycycl Aromat Compd 38:42–50. CrossRefGoogle Scholar
  29. Nguyen TD, Dang CH, Mai DT (2018b) Biosynthesized AgNP capped on novel nanocomposite 2-hydroxypropyl-β-cyclodextrin/alginate as a catalyst for degradation of pollutants. Carbohyd Polym 197:29–37. CrossRefGoogle Scholar
  30. Prakash P, Gnanaprakasam P, Emmanuel R, Arokiyaraj S, Saravanan M (2013) Green synthesis of silver nanoparticles from leaf extract of Mimusops elengi, Linn. for enhanced antibacterial activity against multi drug resistant clinical isolates. Colloids Surf B Biointerfaces 108:255–259. CrossRefGoogle Scholar
  31. Pramanik N, Bhattacharyya A, Kundu PP (2015) Spectroscopic analysis and catalytic application of biopolymer capped silver nanoparticles, an effective antimicrobial agent. J Appl Polym Sci 132(41495). Google Scholar
  32. Qu Y, You S, Zhang X, Pei X, Shen W, Li Z, Li S, Zhang Z (2018) Biosynthesis of gold nanoparticles using cell-free extracts of Magnusiomyces ingens LH-F1 for nitrophenols reduction. Bioprocess Biosyst Eng 41(3):359–367. CrossRefGoogle Scholar
  33. Rafique M, Sadaf I, Rafique MS, Tahir MB (2017) A review on green synthesis of silver nanoparticles and their applications. Artif Cells Nanomed Biotechnol 45:1272–1291. CrossRefGoogle Scholar
  34. Saratale RG, Shin HS, Kumar G, Benelli G, Ghodake GS, Jiang YY, Kim DS, Saratale GD (2018) Exploiting fruit byproducts for eco-friendly nanosynthesis: Citrus × clementina peel extract mediated fabrication of silver nanoparticles with high efficacy against microbial pathogens and rat glial tumor C6 cells. Environ Sci Pollut Res 25:10250–10263. CrossRefGoogle Scholar
  35. Sathishkumar M, Sneha K, Won S, Cho CW, Kim S, Yun YS (2009) Cinnamon zeylanicum bark extract and powder mediated green synthesis of nano-crystalline silver particles and its bactericidal activity. Colloids Surf B Biointerfaces 73:332–338. CrossRefGoogle Scholar
  36. Schrofel A, Kratosova G, Safarik I, Safarikova M, Raska I, Shor LM (2014) Applications of biosynthesized metallic nanoparticles – a review. Acta Biomater 10:4023–4042. CrossRefGoogle Scholar
  37. Sheny D, Mathew J, Philip D (2011) Phytosynthesis of Au, Ag and Au–Ag bimetallic nanoparticles using aqueous extract and dried leaf of Anacardium occidentale. Spectrochim Acta A Mol Biomol Spectrosc 79:254–262. CrossRefGoogle Scholar
  38. Singh P, Kim YJ, Zhang D, Yang DC (2016a) Biological synthesis of nanoparticles from plants and microorganisms. Trends Biotechnol 34:588–599. CrossRefGoogle Scholar
  39. Singh P, Kim YJ, Wang C, Mathiyalagan R, Yang DC (2016b) The development of a green approach for the biosynthesis of silver and gold nanoparticles by using Panax ginseng root extract, and their biological applications. Artif Cells Nanomed Biotechnol 44:1150–1157. CrossRefGoogle Scholar
  40. Singh P, Kim YJ, Yang DC (2015) A strategic approach for rapid synthesis of gold and silver nanoparticles by Panax ginseng leaves. Artif Cells Nanomed Biotechnol 44:1949–1957. CrossRefGoogle Scholar
  41. Tareq FK, Fayzunnesa M, Kabir MS (2017) Antimicrobial activity of plant-median synthesized silver nanoparticles against food and agricultural pathogens. Microb Pathog 109:228–232. CrossRefGoogle Scholar
  42. Tousch D, Bidel LPR, Cazals G, Ferrare K, Leroy J, Faucanie M, Chevassus H, Tournier M, Lajoix AD, Azay-Milhau J (2014) Chemical analysis and antihyperglycemic activity of an original extract from burdock root (Arctium lappa). J Agric Food Chem 62:7738–7745. CrossRefGoogle Scholar
  43. Umamaheswari C, Lakshmanan A, Nagarajan NS (2018) Green synthesis, characterization and catalytic degradation studies of gold nanoparticles against Congo red and methyl orange. J Photochem Photobiol B 178:33–39. CrossRefGoogle Scholar
  44. Wacławek S, Gončuková Z, Adach K, Fijałkowski M, Černík M (2018) Green synthesis of gold nanoparticles using Artemisia dracunculus extract: control of the shape and size by varying synthesis conditions. Environ Sci Pollut Res Int 25(24):24210–24219 CrossRefGoogle Scholar
  45. Zhang S, Tang Y, Vlahovic B (2016) A review on preparation and applications of silver-containing nanofibers. Nanoscale Res Lett, 11 11:80–87. CrossRefGoogle Scholar
  46. Zhao L, Shen G, Ma G, Yan X (2016) Engineering and delivery of nanocolloids of hydrophobic drugs. Adv Colloid Interf Sci 249:308–320. CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institute of Research and DevelopmentDuy Tan UniversityDa Nang CityVietnam
  2. 2.Institute of Chemical TechnologyVietnam Academy of Science and TechnologyHo Chi Minh CityVietnam
  3. 3.Graduate University of Science and TechnologyVietnam Academy of Science and TechnologyHanoiVietnam
  4. 4.School of BiotechnologyTan Tao UniversityLong An ProvinceVietnam
  5. 5.Department of Chemical TechnologyHo Chi Minh City University of Food IndustryHo Chi MinhVietnam

Personalised recommendations