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Aerobic and oxygen-limited enrichment of BTEX-degrading biofilm bacteria: dominance of Malikia versus Acidovorax species

  • Tibor Benedek
  • Flóra Szentgyörgyi
  • István Szabó
  • Balázs Kriszt
  • Fruzsina Révész
  • Júlia Radó
  • Gergely Maróti
  • András Táncsics
Research Article

Abstract

Due to their high resistance against environmental challenges, bacterial biofilms are ubiquitous and are frequently associated with undesired phenomena in environmental industry (e. g. biofouling). However, because of the high phylogenetic and functional diversity, bacterial biofilms are important sources of biotechnologically relevant microorganisms, e.g. those showing bioremediation potential. In our previous work, the high phylogenetic and metabolic diversity of a clogging biofilm, developed in a simple aromatic hydrocarbon (BTEX)-contaminated groundwater well was uncovered. The determination of relationships between different groups of biofilm bacteria and certain metabolic traits has been omitted so far. Therefore, by setting up new biofilm-based enrichment microcosms, the research goal of the present study was to identify the aerobic/hypoxic BTEX-degrading and/or prolific biofilm-forming bacteria. The initial bacterial community composition as well as temporal dynamics due to the selective enrichment has been determined. The obtained results indicated that the concentration of dissolved oxygen may be a strong selective force on the evolution and final structure of microbial communities, developed in hydrocarbon-contaminated environments. Accordingly, members of the genus Malikia proved to be the most dominant community members of the aerobic BTEX-degrading enrichments. Acidovorax spp. dominated the oxygen-limited/hypoxic setup. During the study, a strain collection of 23 different bacterial species was obtained. Non-pathogenic members of this strain collection, with outstanding biodegradation (e.g. Pseudomonas, Variovorax isolates) and biofilm-forming potential (e.g. Rhizobium), may potentially be applied in the development of biofilm-based semipermeable reactive biobarriers.

Keywords

Biodegradation Biobarriers Biofilm BTEX Catechol 2,3-dioxygenases Oxygen-limited 

Notes

Funding information

This work was supported by the ÚNKP-17-4 New National Excellence Program of the Ministry of Human Capacities; by the Economic Development and Innovation Operational Program GINOP-2.1.1-15-2015-00630; and by the Higher Education Institutional Excellence Program (1783-3/2018/FEKUTSTRAT) awarded by the Ministry of Human Capacities within the framework of water related researches of Szent István University.

Supplementary material

11356_2018_3096_MOESM1_ESM.docx (1.3 mb)
ESM 1 (DOCX 1370 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Regional University Centre of Excellence in Environmental IndustrySzent István UniversityGödöllőHungary
  2. 2.Department of Environmental Safety and EcotoxicologySzent István UniversityGödöllőHungary
  3. 3.Institute of Plant BiologyBiological Research Centre of the Hungarian Academy of SciencesSzegedHungary
  4. 4.Faculty of Agricultural and Economics Studies, Tessedik CampusSzent István UniversitySzarvasHungary

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