Microalgae cultivation is a promising approach to remove ambient CO2 via photosynthesis process. This paper investigates the impact of high CO2 concentrations (6, 12, and 16%) on algae growth, CO2 biofixation, lipid and carbohydrate contents, and nutrient removal of newly isolated microalgae, Coelastrum sp. SM. In addition, the ability of microalgae to produce biodiesel at optimal condition was studied. The microalgae were cultivated in wastewater using an airlift photobioreactor. Under 12% CO2, the maximum biomass productivity and CO2 fixation rate were 0.267 g L−1 day−1 and 0.302 g L−1 h−1, respectively. Total Kjeldahl nitrogen (TKN), total phosphorous (TP), nitrate, and sCOD removal efficiency were 84.01, 100, 86.811, and 73.084%, respectively. Under 12% CO2 and at the same condition for cell growth, the highest lipid and carbohydrate contents were 3 7.91 and 58.45%, respectively. The composition of fatty acids methyl ester (FAME) of the microalga lipid was defined. Based on the obtained results and FAME profile, Coelastrum sp. SM was a suitable feedstock for biodiesel production and also, the organism had a great potential for CO2 biofixation, which is also more suitable than any other reported strains in other related studies.
Microalgae CO2 bio-fixation Coelastrum sp. SM Biodiesel Lipid Carbohydrate Nutrient removal
This is a preview of subscription content, log in to check access.
The authors are grateful to Biotechnology Research Lab., Babol Noshirvani University of Technology, for the facilities provided to conduct present research.
This research project was financially support by Iran National Gas Company Mazandaran province under contract no. 11226.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Dineshkumar R, Dash SK, Sen R (2015) Process integration for microalgal lutein and biodiesel production with concomitant flue gas CO2 sequestration: a biorefinery model for healthcare, energy and environment. RSC Adv 5:73381–73394. https://doi.org/10.1039/C5RA09306FCrossRefGoogle Scholar
Dwivedi G, Verma P, Sharma MP (2016) Impact of oil and biodiesel on engine operation in cold climatic condition. J Mater Environ Sci 12:4540–4555Google Scholar
Ho S-H, Chen Y-D, Chang C-Y, Lai YY, Chen CY, Kondo A, Ren NQ, Chang JS (2017) Feasibility of CO2 mitigation and carbohydrate production by microalga Scenedesmus obliquus CNW-N used for bioethanol fermentation under outdoor conditions: effects of seasonal changes. Biotechnol Biofuels 10:27. https://doi.org/10.1186/s13068-017-0712-5CrossRefGoogle Scholar
Lee Y-H, Weng T-C (2018) Development of synthetic perfluorinated photobioreactor system for simultaneous carbon dioxide separation and promotion of microalgae growth and productions. J Chem Technol Biotechnol 93:1065–1074. https://doi.org/10.1002/jctb.5463CrossRefGoogle Scholar
Ruangsomboon S, Prachom N, Sornchai P (2017) Enhanced growth and hydrocarbon production of Botryococcus braunii KMITL 2 by optimum carbon dioxide concentration and concentration-dependent effects on its biochemical composition and biodiesel properties. Bioresour Technol 244:1358–1366. https://doi.org/10.1016/j.biortech.2017.06.042CrossRefGoogle Scholar
Talebi AF, Mohtashami SK, Tabatabaei M, Tohidfar M, Bagheri A, Zeinalabedini M, Hadavand Mirzaei H, Mirzajanzadeh M, Malekzadeh Shafaroudi S, Bakhtiari S (2013) Fatty acids profiling: a selective criterion for screening microalgae strains for biodiesel production. Algal Res 2:258–267. https://doi.org/10.1016/j.algal.2013.04.003CrossRefGoogle Scholar
Wu K-c, Ho K-c, Tang C-c, Yau Y-h (2018) The potential of foodwaste leachate as a phycoremediation substrate for microalgal CO2 fixation and biodiesel production. Environ Sci Pollut Res. https://doi.org/10.1007/s11356-018-1242-9