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Environmental Science and Pollution Research

, Volume 25, Issue 30, pp 30191–30198 | Cite as

Dynamic transport of antibiotics and antibiotic resistance genes under different treatment processes in a typical pharmaceutical wastewater treatment plant

  • Linxuan Li
  • Changsheng Guo
  • Shisuo Fan
  • Jiapei Lv
  • Yan Zhang
  • Yan Xu
  • Jian Xu
Research Article
  • 107 Downloads

Abstract

The propagation of antibiotic resistance is a challenge for human health worldwide, which has drawn much attention on the reduction of the resistance genes. To understand their occurrence during different treatment processes, in this study, four classes of antibiotics (tetracyclines, sulfonamides, quinolones, and macrolides), eight antibiotic resistance genes (ARGs) (tetB, tetW, sul1, sul2, gyrA, qepA, ermB, and ermF), and two mobile elements (int1 and int2) were investigated in a typical pharmaceutical plant. The total concentrations of antibiotics were detected in the range of 2.6 × 102 to 2.5 × 103 ng/L in the treatment processes, and the high abundance of ARGs was detected in the biological treatment unit. The dynamic trend analysis showed that antibiotics were partially removed in the anaerobic/aerobic processes, where ARGs were proliferated. The abundance of tetB and gyrA genes was positively correlated with pH and EC (p < 0.05), and the tetW, sul1 and sul2 genes were significantly correlated with TOC, TN, and DO (p < 0.05), indicating the influence of physicochemical properties of the solution on the levels of ARG subtypes. The phylogenetic analysis showed that the tetW clones had high homology with some pathogenic microorganisms, such as Klebsiella pneumonia and Neisseria meningitides, which would threaten human health. Results indicated that the horizontal transfer acted as a major driver in the ARGs evolution.

Keywords

Antibiotics Antibiotic resistance genes Pharmaceutical plant Dynamic transport 

Notes

Acknowledgements

This work was supported by State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences.

References

  1. Bai Y, Meng W, Xu J, Zhang Y, Guo C (2014) Occurrence, distribution and bioaccumulation of antibiotics in Liao River basin in China. Environ Sci Proc Impacts 16(3):586CrossRefGoogle Scholar
  2. Batt AL, Bruce IB, Aga DS (2006) Evaluating the vulnerability of surface waters to antibiotic contamination from varying wastewater treatment plant discharges. Environ Pollut 142:295–302CrossRefGoogle Scholar
  3. Batt AL, Kim S, Aga DS (2007) Comparison of the occurrence of antibiotics in four full-scale wastewater treatment plants with varying designs and operations. Chemosphere 68:428–435CrossRefGoogle Scholar
  4. Ben W, Wang J, Cao R, Yang M, Zhang Y, Qiang Z (2017) Distribution of antibiotic resistance in the effluents of ten municipal wastewater treatment plants in China and the effect of treatment processes. Chemosphere 172:392–398CrossRefGoogle Scholar
  5. Bouki C, Venieri D, Diamadopoulos E (2013) Detection and fate of antibiotic resistant bacteria in wastewater treatment plants: a review. Ecotox Environ Safe 91:1–9CrossRefGoogle Scholar
  6. Brunsch AF, ter Laak TL, Rijnaarts H, Christoffels E (2018) Pharmaceutical concentration variability at sewage treatment plant outlets dominated by hydrology and other factors. Environ Pollut 235:615–624CrossRefGoogle Scholar
  7. Carstens A, Bartie C, Dennis R, Bezuidenhout C (2014) Antibiotic-resistant heterotrophic plate count bacteria and amoeba-resistant bacteria in aquifers of the Mooi River, north west province, South Africa. J Water Health 12:835–845CrossRefGoogle Scholar
  8. Chang XS, Meyer MT, Liu XY, Zhao Q, Chen H, Chen JA, Qiu ZQ, Yang L, Cao J, Shu WQ (2010) Determination of antibiotics in sewage from hospitals, nursery and slaughter house, wastewater treatment plant and source water in Chongqing region of Three Gorge Reservoir in China. Environ Pollut 158:1444–1450CrossRefGoogle Scholar
  9. Chen H, Zhang M (2013) Effects of advanced treatment systems on the removal of antibiotic resistance genes in wastewater treatment plants from Hangzhou, China. Environ Sci Technol 47:8157–8163Google Scholar
  10. Christgen B, Yang Y, Ahammad SZ, Li B, Rodriquez DC, Zhang T, Graham DW (2016) Metagenomics shows that low-energy anaerobic−aerobic treatment reactors reduce antibiotic resistance gene levels from domestic wastewater. Environ Sci Technol 49:2577–2584CrossRefGoogle Scholar
  11. Dang B, Mao D, Xu Y, Luo Y (2017) Conjugative multi-resistant plasmids in Haihe River and their impacts on the abundance and spatial distribution of antibiotic resistance genes. Water Res 111:81–91CrossRefGoogle Scholar
  12. Gao P, Munir M, Xagoraraki I (2012) Correlation of tetracycline and sulfonamide antibiotics with corresponding resistance genes and resistant bacteria in a conventional municipal wastewater treatment plant. Sci Total Environ 421:173–183CrossRefGoogle Scholar
  13. Guo X, Yan Z, Zhang Y, Kong X, Kong D, Shan Z, Wang N (2017) Removal mechanisms for extremely high-level fluoroquinolone antibiotics in pharmaceutical wastewater treatment plants. Environ Sci Pollut R 24:8769–8777CrossRefGoogle Scholar
  14. He LY, Liu YS, Su HC, Zhao JL, Liu SS, Chen J, Liu WR, Ying GG (2014) Dissemination of antibiotic resistance genes in representative broiler feedlots environments: identification of indicator ARGs and correlations with environmental variables. Environ Sci Technol 48:13120–13129CrossRefGoogle Scholar
  15. He LY, Ying GG, Liu YS, Su HC, Chen J, Liu SS, Zhao J-L (2016) Discharge of swine wastes risks water quality and food safety: antibiotics and antibiotic resistance genes from swine sources to the receiving environments. Environ Int 92:210–219CrossRefGoogle Scholar
  16. Hellstrm D, Jonsson L (2004) Evaluation of small wastewater treatment systems. Water Sci Technol 48:61–68CrossRefGoogle Scholar
  17. Hu J, Zhou J, Zhou S, Wu P, Tsang YF (2018) Occurrence and fate of antibiotics in a wastewater treatment plant and their biological effects on receiving waters in Guizhou. Process Saf Enviro 113:483–490CrossRefGoogle Scholar
  18. LaPara TM, Burch TR, McNamara PJ, Tan DT, Yan M, Eichmiller JJ (2011) Tertiary-treated municipal wastewater is a significant point source of antibiotic resistance genes into Duluth-Superior Harbor. Environ Sci Technol 45:9543–9549CrossRefGoogle Scholar
  19. Li D, Yang M, Hu JY, Zhang J, Liu RY, Gu X, Zhang Y, Wang ZY (2009) Antibiotic-resistance profile in environmental bacteria isolated from penicillin production wastewater treatment plant and the receiving river. Environ Microbio 11(6):1506–1517CrossRefGoogle Scholar
  20. Li D, Yu T, Zhang Y, Yang M, Li Z, Liu M, Qi R (2010) Antibiotic resistance characteristics of environmental bacteria from an oxytetracycline production wastewater treatment plant and the receiving river. Appl Environ Micro 76:3444–3451CrossRefGoogle Scholar
  21. Liang H, Gao M, Liu J, Wei Y, Guo X (2010) A novel integrated step-feed biofilm process for the treatment of decentralized domestic wastewater in rural areas of China. J Environ Sci 22:321–327CrossRefGoogle Scholar
  22. Liu M, Zhang Y, Yang M, Tian Z, Ren L, Zhang S (2012) Abundance and distribution of tetracycline resistance genes and mobile elements in an oxytetracycline production wastewater treatment system. Environ Sci Technol 46:7551–7557CrossRefGoogle Scholar
  23. Mao DQ, Yu S, Rysz M, Luo Y, Yang F, Li FX, Hou J, Mu QH, Alvarez PJ (2015) Prevalence and proliferation of antibiotic resistance genes in two municipal wastewater treatment plants. Water Res 85(6):458–466CrossRefGoogle Scholar
  24. Mu Q, Li J, Sun Y, Mao D, Wang Q, Luo Y (2015) Occurrence of sulfonamide-, tetracycline-, plasmid-mediated quinolone- and macrolide-resistance genes in livestock feedlots in northern China. Environ Sci Pollut R 22:6932–6940CrossRefGoogle Scholar
  25. Munir M, Wong K, Xagoraraki I (2011) Release of antibiotic resistant bacteria and genes in the effluent and biosolids of five wastewater utilities in Michigan. Water Res 45:681–693CrossRefGoogle Scholar
  26. Oh HK, Park JH (2009) Characteristics of antibiotic resistant bacteria in urban sewage and river. Korean Society of Environmental Engineering 31:232–239Google Scholar
  27. Pei R, Kim SC, Carlson KH, Pruden A (2006) Effect of river landscape on the sediment concentrations of antibiotics and corresponding antibiotic resistance genes (ARG). Water Res 40:2427–2435CrossRefGoogle Scholar
  28. Pérez S, Eichhorn P, Aga DS (2005) Evaluating the biodegradability of sulfamethazine, sulfamethoxazole, sulfathiazole, and trimethoprim at different stages of sewage treatment. Environ Toxical Chem 24:1361–1367CrossRefGoogle Scholar
  29. Pruden A, Arabi M, Storteboom HN (2012) Correlation between upstream human activities and riverine antibiotic resistance genes. Environ Sci Technol 46:11541–11549CrossRefGoogle Scholar
  30. Rousk J, Brookes PC, Bååth E (2010) Investigating the mechanisms for the opposing pH relationships of fungal and bacterial growth in soil. Soil Biol Biochem 42:926–934CrossRefGoogle Scholar
  31. Sköld O (2000) Sulfonamide resistance: mechanisms and trends. Drug Resist Updates 3:155–160CrossRefGoogle Scholar
  32. Su HC, Ying GG, Tao R, Zhang RQ, Zhao JL, Liu YS (2012) Class 1 and 2 integrons, sul resistance genes and antibiotic resistance in Escherichia coli isolated from Dongjiang River, South China. Environ Pollut 169:42–49CrossRefGoogle Scholar
  33. Su JQ, Wei B, Ou-Yang WY, Huang FY, Zhao Y, Xu HJ, Zhu YG (2015) Antibiotic Resistome and its association with bacterial communities during sewage sludge composting. Environ Sci Technol 49:7356–7363CrossRefGoogle Scholar
  34. Subbiah M, Mitchell SM, Ullman JL, Call DR (2011) β-Lactams and florfenicol antibiotics remain bioactive in soils while ciprofloxacin, neomycin, and tetracycline are neutralized. Appl Environ Micro 77:7255–7260CrossRefGoogle Scholar
  35. Tahrani L, Van LJ, Ben MH, Reyns T (2016) Occurrence of antibiotics in pharmaceutical industrial wastewater, wastewater treatment plant and sea waters in Tunisia. J Water Health 14:208–213CrossRefGoogle Scholar
  36. Tong J, Lu X, Zhang J, Sui Q, Wang R, Chen M, Wei Y (2017) Occurrence of antibiotic resistance genes and mobile genetic elements in enterococci and genomic DNA during anaerobic digestion of pharmaceutical waste sludge with different pretreatments. Bioresour Technol 235:316–324CrossRefGoogle Scholar
  37. Wang FH, Qiao M, Lv ZE, Guo GX, Jia Y, Su YH, Zhu YG (2014) Impact of reclaimed water irrigation on antibiotic resistance in public parks, Beijing, China. Environ Pollut 184:247–253CrossRefGoogle Scholar
  38. Wang J, Mao D, Mu Q, Luo Y (2015) Fate and proliferation of typical antibiotic resistance genes in five full-scale pharmaceutical wastewater treatment plants. Sci Total Environ 526:366–373CrossRefGoogle Scholar
  39. Wang XL, Yang FX, Zhao J, Xu Y, Mao DQ, Zhu X, Luo Y, Alvarez PJJ (2018) Bacterial exposure to ZnO nanoparticles facilitates horizontal transfer of antibiotic resistance genes. NanoImpact 10:61–67CrossRefGoogle Scholar
  40. Wu N, Qiao M, Zhang B, Cheng WD, Zhu YG (2010) Abundance and diversity of tetracycline resistance genes in soils adjacent to representative swine feedlots in China. Environ Sci Technol 44:6933–6939CrossRefGoogle Scholar
  41. Xu J, Xu Y, Wang HM, Guo CS, Qiu H, He Y (2015) Occurrence of antibiotics and antibiotic resistance genes in a sewage treatment plant and its effluent-receiving river. Chemosphere 119:1379–1385CrossRefGoogle Scholar
  42. Xu Y, Guo CS, Luo Y, Lv JP, Zhang Y, Lin HX, Xu J (2016) Occurrence and distribution of antibiotics, antibiotic resistance genes in the urban rivers in Beijing, China. Environ Pollut 213:833–840CrossRefGoogle Scholar
  43. Xu Y, Xu J, Mao D, Luo Y (2017) Effect of the selective pressure of sub-lethal level of heavy metals on the fate and distribution of ARGs in the catchment scale. Environ Pollut 220:900–908CrossRefGoogle Scholar
  44. Yang F, Huang L, Li L, Yang Y, Mao D, Luo Y (2017) Discharge of KPC-2 genes from the WWTPs contributed to their enriched abundance in the receiving river. Sci Total Environ 581-582:136–143CrossRefGoogle Scholar
  45. Zhai W, Yang F, Mao D, Luo Y (2016) Fate and removal of various antibiotic resistance genes in typical pharmaceutical wastewater treatment systems. Environ Sci Pollut R 23(12):12020–12038CrossRefGoogle Scholar
  46. Zhang T, Zhang M, Zhang X, Fang HH (2009a) Tetracycline resistance genes and tetracycline resistant lactose-fermenting Enterobacteriaceae in activated sludge of sewage treatment plants. Environ Sci Technol 43:3455–3460CrossRefGoogle Scholar
  47. Zhang XX, Wu B, Zhang Y, Zhang T, Yang LY, Fang HHP, Ford T, Cheng SP (2009b) Class 1 integronase gene and tetracycline resistance genes teta and tetc in different water environments of Jiangsu province, China. Ecotoxicology 18(6):652–660CrossRefGoogle Scholar
  48. Zhang QQ, Ying GG, Pan CG, Liu YS, Zhao JL (2015) Comprehensive evaluation of antibiotics emission and fate in the river basins of China: source analysis, multimedia modeling, and linkage to bacterial resistance. Environ Sci Technol 49(11):6772–6782CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Linxuan Li
    • 1
  • Changsheng Guo
    • 2
  • Shisuo Fan
    • 1
  • Jiapei Lv
    • 2
  • Yan Zhang
    • 2
  • Yan Xu
    • 2
    • 3
  • Jian Xu
    • 2
  1. 1.School of Resources and EnvironmentAnhui Agricultural UniversityHefeiChina
  2. 2.State Key Laboratory of Environmental Criteria and Risk AssessmentChinese Research Academy of Environmental SciencesBeijingChina
  3. 3.Agro-Environmental Protection InstituteMinistry of AgricultureTianjinChina

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