Environmental Science and Pollution Research

, Volume 25, Issue 27, pp 26927–26938 | Cite as

Diesel oil removal by Serratia sp. W4-01 immobilized in chitosan-activated carbon beads

  • Chanokporn Muangchinda
  • Chalinee Chamcheun
  • Rajitpitch Sawatsing
  • Onruthai PinyakongEmail author
Research Article


Serratia sp. W4-01 was immobilized in chitosan-activated carbon beads and used for diesel oil removal. The type and concentration of chitosan, activated carbon content, and bead diameter were investigated as factors affecting diesel oil removal. The results showed that 2% (w/v) squid pen chitosan beads modified with 1% activated carbon (w/v) and with a 3-mm diameter had a good spherical shape and strength as well as diesel oil removal capability. The immobilized W4-01 cells removed more than 40% of diesel oil after 7 days when the initial diesel oil concentration was 100 to 400 mg L−1, whereas 29–36% of diesel oil was removed after 14 days when the initial concentration was 800 to 1000 mg L−1. Additionally, the immobilized cells maintained the ability to remove diesel oil over a pH range of 5–11. The addition of a biosurfactant increased the diesel oil removal from 62 to 75%. The reusability tests revealed that the ability of immobilized cells to remove diesel oil was enhanced after reuse, and 50–90% of diesel oil was removed during 2 to 12 reuse cycles. The stability and survival of W4-01 cells was confirmed by scanning electron microscopy and confocal laser scanning microscopy. The results of this study showed the potential use of W4-01 cells immobilized in chitosan-activated carbon beads for future applications in remediating diesel contamination.


Diesel oil Chitosan beads Activated carbon Immobilization Bioremediation 


Funding information

This research was funded by Chulalongkorn University (R/F_2557_019_08_23).

Supplementary material

11356_2018_2742_MOESM1_ESM.docx (207 kb)
ESM 1 (DOCX 206 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Microbial Technology for Marine Pollution Treatment Research Unit, Faculty of ScienceChulalongkorn UniversityBangkokThailand
  2. 2.Department of Microbiology, Faculty of ScienceChulalongkorn UniversityBangkokThailand
  3. 3.Research Program on Remediation Technologies for Petroleum ContaminationCenter of Excellence on Hazardous Substance Management (HSM)BangkokThailand

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