Metal release and sequestration from black slate mediated by a laccase of Schizophyllum commune

  • Julia Kirtzel
  • Eric Leon Scherwietes
  • Dirk Merten
  • Katrin Krause
  • Erika Kothe
New Toxic Emerging Contaminants: Beyond the Toxicological effects


Schizophyllum commune is a filamentous basidiomycete which can degrade complex organic macromolecules like lignin by the secretion of a large repertoire of enzymes. One of these white rot enzymes, laccase, exhibits a broad substrate specificity and is able to oxidize a variety of substances including carbonaceous rocks. To investigate the role of laccase in bioweathering, laccase gene lcc2 was overexpressed, and the influence on weathering of black slate, originating from a former alum mine in Schmiedefeld, Germany, was examined. The metal release from the rock material was enhanced, associated with a partial metal accumulation into the mycelium. A sequestration of metals could be shown with fluorescent staining methods, and an accumulation of Zn, Cd, and Pb was visualized in different cell organelles. Additionally, we could show an increased metal resistance of the laccase overexpressing strain.


Schizophyllum commune Multicopper oxidases Metal release Stress resistance Black slate Bioweathering 



This project was supported by the Deutsche Forschungsgesellschaft through GRK 1257 and JSMC.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11356_2018_2568_MOESM1_ESM.pdf (88 kb)
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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institute of Microbiology, Microbial CommunicationFriedrich Schiller UniversityJenaGermany
  2. 2.Institute of Geosciences, Applied GeologyFriedrich Schiller UniversityJenaGermany

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