Characterization of a di-n-butyl phthalate-degrading bacterial consortium and its application in contaminated soil

  • Jing Yang
  • Chuling Guo
  • Shasha Liu
  • Weiting Liu
  • Han Wang
  • Zhi Dang
  • Guining Lu
Research Article
  • 39 Downloads

Abstract

Dibutyl phthalate (DBP), as a plasticizer, is widely used in China, and it is easily released into diverse environments. In this study, we have obtained a stable bacterial consortium (B1) enriched from municipal sewage treatment plant activated sludge. The obtained bacterial consortium B1 was capable of degrading DBP and was mainly composed of Pandoraea sp. and Microbacterium sp. From the initial concentrations of 35–500 mg L−1, DBP was efficiently degraded by the consortium, with the degradation rates above 92% within 3 days. The optimal temperature for DBP degradation was 30 °C and consortium B1 could adapt to a wide range of pH (5.5–8.5). The analysis of Illumina sequencing further showed that the relative abundance of Pandoraea was increased at the beginning of the degradation, while Microbacterium was decreased. In the later stage of the degradation, the change of the relative abundance of Pandoraea and Microbacterium was opposite. Apart from DBP, consortium B1 could also utilize dimethyl phthalate (DMP), di-2-ethylhexyl phthalate (DEHP), and phthalic acid (PA) as the sole carbon. Moreover, adding B1 to DBP-contaminated soil could greatly improve the removal rate of DBP, suggesting that B1 has a great potential for the bioremediation of DBP-contaminated environments.

Keywords

Di-n-butyl phthalate Bacterial consortium Biodegradation Degradation pathway Illumina sequencing Soil bioremediation 

Supplementary material

11356_2018_1862_MOESM1_ESM.pdf (365 kb)
ESM 1 (PDF 343 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Jing Yang
    • 1
  • Chuling Guo
    • 1
    • 2
    • 3
  • Shasha Liu
    • 1
  • Weiting Liu
    • 1
  • Han Wang
    • 1
  • Zhi Dang
    • 1
    • 2
  • Guining Lu
    • 1
    • 2
  1. 1.School of Environment and EnergySouth China University of TechnologyGuangzhouPeople’s Republic of China
  2. 2.The Key Lab of Pollution Control and Ecosystem Restoration in Industry ClustersMinistry of EducationGuangzhouPeople’s Republic of China
  3. 3.Guangdong Provincial Engineering and Technology Research Center for Environmental Risk Prevention and Emergency DisposalSouth China University of TechnologyGuangzhouChina

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