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Effects of cadmium exposure on antioxidant enzymes and histological changes in the mud shrimp Austinogebia edulis (Crustacea: Decapoda)

  • Shagnika Das
  • Li-Chun Tseng
  • Chi Chou
  • Lan Wang
  • Sami Souissi
  • Jiang-Shiou HwangEmail author
Research Article
  • 64 Downloads

Abstract

The trace metal cadmium (Cd) is a toxic pollutant known to induce oxidative stress and other sublethal to lethal effects on aquatic organisms. We exposed the marine mud shrimp Austinogebia edulis to Cd concentrations of 0, 0.5, and 5 mg/kg for up to 4 days (24, 48, 72, 96 h). We studied the activity of antioxidant enzymes in the hepatopancreas, gill, and muscle of A. edulis. Antioxidant enzymes (SOD, CAT, and GPx) decreased with increasing Cd concentration and extended exposure time in these three organs of the mud shrimp A. edulis. Increasing Cd concentration led to an increase in ROS and resulted ultimately in membrane lipid peroxidation at higher Cd concentrations. Significant damage of the hepatopancreas of A. edulis was noticed at higher concentrations of Cd, showing damages like the disappearance of epithelial cell boundaries, detachment of cells from the basal lamina, cellular swelling, necrosis, and reduction of glycogen. In conclusion, Cd caused oxidative damage by reducing the activities of antioxidant enzymes and by damaging the tissue structure in major organs of the mud shrimp A. edulis.

Keywords

Oxidative damage Cadmium Antioxidant enzyme Mud shrimp Austinogebia edulis Hepatopancreas Gill Muscle 

Notes

Acknowledgements

We thank the College of Life Science of Shanxi University, China, for providing the necessary facilities and equipment for carrying out this study. Finally, we thank Mr. Chih-Ming Lin and Dr. Shao-Hung Peng for their assistance during the field sampling. This paper is a contribution to the International Joint Laboratory between Lille University and Taiwan.

Funding information

We received from the Ministry of Science and Technology (MOST) of Taiwan financial support through grant nos. MOST 104-2611-M-019- 004, MOST 105-2621-M-019-001, MOST 105-2918-I-019-001 and MOST 106-2621-M-019- 001, and MOST 107-2621-M-019-001 to J.-S. Hwang, as well as the grant nos. MOST 104-2811-M-019-005, MOST 105-2811-M-019-008, MOST 106-2811-M-019-004, and MOST 107-2811-M-019-004 to L.-C. Tseng.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Shagnika Das
    • 1
    • 2
  • Li-Chun Tseng
    • 1
  • Chi Chou
    • 3
  • Lan Wang
    • 4
  • Sami Souissi
    • 2
  • Jiang-Shiou Hwang
    • 1
    • 5
    Email author
  1. 1.Institute of Marine BiologyNational Taiwan Ocean UniversityKeelungTaiwan
  2. 2.LOG, Laboratoire d’Océanologie et de GéosciencesUniversity of Lille, CNRS, Universite Littoral Cote d’Opale, UMR 8187WimereuxFrance
  3. 3.Department of PathologyMackay Memorial HospitalNew Taipei CityTaiwan
  4. 4.School of Life SciencesShanxi UniversityTaiyuanChina
  5. 5.Center of Excellence for the OceansNational Taiwan Ocean UniversityKeelungTaiwan

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