Environmental Science and Pollution Research

, Volume 26, Issue 10, pp 9851–9860 | Cite as

Effect of EDTA and NTA on cadmium distribution and translocation in Pennisetum purpureum Schum cv. Mott

  • Aekkacha Tananonchai
  • Pantawat SampanpanishEmail author
  • Penradee Chanpiwat
  • Somchai Tancharakorn
  • Usa Sukkha
Research Article


The primary objective of this research was to investigate the cadmium (Cd) distribution in Pennisetum purpurem (Napier grass) in the presence of 30 mg/L of Cd and different types and concentrations of chelating agents (ethylenediaminetetraacetic acid disodium dihydrate (EDTA), nitrilotriacetic acid (NTA), and EDTA-NTA mixtures). Plant samples were collected every 15 d during a 105-d experimental period. Accumulation of Cd in each part of the plant was determined using atomic absorption spectrometer (AAS), and the distribution of Cd was determined by laser ablation inductively coupled plasma mass spectrometer (LA-ICP-MS) and synchrotron radiation micro X-ray fluorescence (SR-micro-XRF). The highest concentrations of Cd accumulation of 889 ± 53 mg kg−1 in the underground part (roots) and 265 ± 26 mg kg−1 in the aboveground part (stems and leaves) in the presence of 1:1 M ratio of Cd:EDTA after 30 d of exposure were observed. Plants grown in the presence of either NTA or EDTA-NTA mixtures showed significant lower Cd accumulation levels. The LA-ICP-MS analysis showed that Cd was primarily accumulated in the aboveground part (stems and leaves), especially in the xylem and intercalary meristem. In addition, translocation factor was very low. Thus, P. purpurem could be considered as a candidate plant for cadmium phytostabilization.


Napier grass Mechanism Transportation Distribution Laser ablation inductively coupled plasma mass spectrometry Synchrotron X-ray fluorescence 



We would like to express our sincere thanks to the Environmental Research Institute (ERIC), the Center of Excellence on Hazardous Substance Management (HSM), Chulalongkorn University and the Synchrotron Light Research Institute (SLRI), for their invaluable support in terms of facilities and scientific equipment.

Funding information

This study received financial support from the Office of Higher Education Commission (OHEC) and the S&T Postgraduate Education and Research Development Office (PERDO) for the research program and the 90th Anniversary of Chulalongkorn University Fund (Ratchadaphiseksomphot Endowment Fund) and the Ratchadaphiseksomphot Endowment Fund, Chulalongkorn University, for the research unit.


  1. Abdel Salam MA (2012) Chemical and phytoremediation of clayey and sandy textured soils polluted with cadmium. Am Eurasian J Agric Environ Sci 12(6):689–693. CrossRefGoogle Scholar
  2. Abrantes S, Amaral ME, Costa AP, Duarte AP (2007) Hydrogen peroxide bleaching of Arundo donax L. kraft-anthraquinone pulp - effect of a chelating stage. Ind Crop Prod 25(3):288–293. CrossRefGoogle Scholar
  3. Aisien FA, Oboh IO, Aisien ET (2012) Phytotechnology-remediation of inorganic contaminants. In: Anjum NA, Pereira MA, Ahmad I, Duarte AC, Umar S, Khan NA (eds) Phytotechnology remediation of environmental contaminants. CRC Press, Boca Raton, pp 75–82CrossRefGoogle Scholar
  4. Akkajit P (2015) Review of the current situation of Cd contamination in agricultural field in the Mae Sot district, Tak province, northwestern Thailand. Appl Environ Res 37(1):71–82. CrossRefGoogle Scholar
  5. Alloway BJ (2011) Cadmium. Heavy metals in soils, Chapman & Hall, LondonGoogle Scholar
  6. Anjum NA, Pereira ME, Ahmad I, Duarte AC, Umar S, Khan NA (2013) Phytotechnologies: remediation of environmental contaminants. Int J Environ Anal Chem 93(14):1557–1558. CrossRefGoogle Scholar
  7. Assawadithalerd M, Siangliw M, Tongcumpou C (2014) Effects of organic fertilizer on Cd bioavailability and Cd accumulation in rice grown in contaminated paddy soil. Appl Environ Res 36(3):95–104. CrossRefGoogle Scholar
  8. Becker JS, Zoriy M, Matusch A, Wu B, Salber D, Palm C (2010) Bioimaging of metals by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Mass Spectrometry Review 29(1):156–175. CrossRefGoogle Scholar
  9. Brown JC, Von D, Jolley C, Lytle MC (1991) Comparative evaluation of iron solubilizing substances (phytosiderophores) released by oats and corn: iron-efficient and iron-inefficient plants. Plant Soil 130:157–163. CrossRefGoogle Scholar
  10. Cheng S (2003) Effects of heavy metals on plants and resistance mechanisms. Environ Sci Pollut Res 10(4):256–264. CrossRefGoogle Scholar
  11. Eissa MA (2016) Effect of sugarcane vinasse and EDTA on cadmium phytoextraction by two saltbush plants. Environ Sci Pollut Res 23(10):10247–10254. CrossRefGoogle Scholar
  12. Eissa MA (2017) Phytoextraction mechanism of Cd by Atriplex lentiformis using some mobilizing agents. Ecol Eng 108(2017):220–226. CrossRefGoogle Scholar
  13. Ensley BD (2000) Rationale for use of phytoremediation. In: Raskin I, Ensley BD (eds) Phytoremediation of toxic metals using plants to clean up the environment, vol 76. John Wiley and Sons, New York, pp 312–313. CrossRefGoogle Scholar
  14. Evangelou M, Ebel M, Schaeffer A (2007) Chelate assisted phytoextraction of heavy metals from soil. Effect, mechanism, toxicity and fate of chelating agents. Chemosphere 68(6):989–1003. CrossRefGoogle Scholar
  15. Fernandez JG (2012) Distribution and quantification in plants leaves by LA-ICP-MS. Dissertation, University of Oviedo and University of Pau and Pays de L’AdourGoogle Scholar
  16. Fukuda N, Hokura A, Kitajima N, Terada Y, Saito H, Abed T, Nakai I (2008) Micro x-ray fluorescence imaging and micro x-ray absorption spectroscopy of cadmium hyper-accumulating plant, Arabidopsis halleri ssp. gemmifera, using high-energy synchrotron radiation. J Anal At Spectrom 23:1068–1075. CrossRefGoogle Scholar
  17. Hamilton JS, Gorishek EL, Mach PM, Sturtevant D, Ladage ML, Suzuki N, Padilla PA, Mittler R, Chapman KD, Verbeck GF (2016) Evaluation of a custom single peltier-cooled ablation cell for elemental imaging of biological samples in laser ablation-inductively coupled plasma mass spectrometry (LA-ICP-MS). J Anal At Spectrom 31(4):1030–1033. CrossRefGoogle Scholar
  18. Hernandez AJ, Carlos G, Oihana B, Jose MB (2006) EDTA-induced heavy metal accumulation and phytotoxicity in cardoon plants. Environ Exp Bot 60(1):26–32. CrossRefGoogle Scholar
  19. Hirzel J, Retamal-Salgado J, Walter I, Matus I (2017) Cadmium accumulation and distribution in plants of three durum wheat cultivars under different agricultural environments in Chile. J Soil Water Conserv 72(1):77–88. CrossRefGoogle Scholar
  20. Ishii Y, Ito K, Fukuyama K (2000) Effect of several cultivation factors on the overwintering ability of napier grass in the southern Kyushu. Jpn J Crop Sci 69(2):209–216. CrossRefGoogle Scholar
  21. Jalil A, Selles F, Clarke JM (1994) Effect of cadmium on growth and the uptake of cadmium and other elements by durum wheat. J Plant Nutr 17:1839–1858. CrossRefGoogle Scholar
  22. Jarvis SC, Jones LHP, Hopper MJ (1976) Cadmium uptake from solution by plants and its transport from roots to shoots. Plant Soil J 44:179–191. CrossRefGoogle Scholar
  23. Kolodynska D (2011) Chelating agents of a new generation as an alternative to conventional chelators for heavy metal ions removal from different waste waters, in: Robert YN (Ed) desalination, In Tech, pp 340-370.
  24. Krishna R (2008) Physical and chemical groundwater remediation technologies. In: Darnault CJG (ed) Overexploitation and contamination of shared groundwater resources. Springer Science, Netherlands, pp 257–274. CrossRefGoogle Scholar
  25. Lefevre I, Mikus KV, Jeromel L, Vavpetic P, Planchon S, Arcon I, Elteren JTV, Lepoint G, Gobert S, Renaut J, Pelicon P, Lutts S (2014) Differential cadmium and zinc distribution in relation to their physiological impact in the leaves of the accumulating Zygophyllum fabago L. Plant Cell Environ 2014(37):1299–1320. CrossRefGoogle Scholar
  26. Lu X, Kruatrachue M, Pokethitiyook P, Homyok K (2004) Removal of cadmium and zinc by water hyacinth Eichhornia crassipes. ScienceAsia 2004(30):93–103. CrossRefGoogle Scholar
  27. Muhammad D, Chen F, Zhao J, Zang G, Wu F (2009) Comparison of EDTA and citric acid enhanced phytoextraction of heavy metals in artificially metal contaminated soil by Typha angustifolia. International Journal of Phytoremediation 11(6):558–574. CrossRefGoogle Scholar
  28. National Environment Board (1994) Determine surface water quality standards [in Thai]. Announcement of the National Environment Board No. 8 in the Royal Thai Government Gazette, no. 111, part 16Google Scholar
  29. Natural Resources Infrastructure and Disasters Management Research Center (2017) The remediation of cadmium in soil and stream sediment in Mae Tao sub-district, Maesot district, Tak province [in Thai]. Accessed 26 July 2017
  30. Neugschwandtner RW, Tlustos P, Komarek M, Szakova J (2008) Phytoextraction of Pb and Cd from a contaminated agricultural soil using different EDTA application regimes: laboratory versus field scale measures of efficiency. Geoderma 144:446–454. CrossRefGoogle Scholar
  31. Pangta S (2009) Use of Ananas comosus (L.) Merr. as indicator for toxicity of chromium and lead in contaminated soil. Dissertation, Chulalongkorn UniversityGoogle Scholar
  32. Peer AW, Baxter IR, Richards EL, Freeman JL, Murphy AS (2007) Phytoremediation and hyperaccumulator plants. In molecular biology of metal homeostasis and detoxification. Springer Science 14:299–340. CrossRefGoogle Scholar
  33. Pendias KA, Pendias H (2001) Trace element in soils and plants, 3rd edn. CRC press, New YorkGoogle Scholar
  34. Pozebon D, Schefflera GL, Dresslerb VL (2017) Recent applications of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) for biological sample analysis: a follow-up review. J Anal At Spectrom 32(5):890–919. CrossRefGoogle Scholar
  35. Radulescu C, Stihi C, Popescu IV, Dulama ID, Chelarescu ED, Chilian A (2013) Heavy metal accumulation and translocation in different parts of Brassica oleracea L. Rom J Phys 58(9–10):1337–1354.
  36. Sabeen M, Mahmood Q, Muhammad I, Fareed I, Khan A, Farid U, Sobia T (2013) Cadmium phytoremediation by Arundo donax L. from contaminated soil and water. Biomed Res Int 9:1–9. CrossRefGoogle Scholar
  37. Salt DE, Prince RC, Pickering LJ, Raskin L (1995) Mechanisms of cadmium mobility and accumulation in Indian mustard. Plant Physiol 109:1427–1433. CrossRefGoogle Scholar
  38. Sampanpanish P (2015) Phytoremediation [in Thai], 1st edn. Chulalongkorn University, ThailandGoogle Scholar
  39. Schor-Fumbarov T, Goldsbrough PB, Adam Z, Tel-Or E (2005) Characterization and expression of a metallothionein gene in the aquatic fern Azolla filiculoides under heavy metal stress. Planta Journal 2005(223):69–76. CrossRefGoogle Scholar
  40. Song Y, Jin L, Wang XJ (2017) Cadmium absorption and transportation pathways in plants. Int J Phytoremediation 19(2):133–141. CrossRefGoogle Scholar
  41. Sun Y, Zhou Q, Liu W, An J, Xu Z, Wang L (2009) Joint effects of arsenic andcadmium on plant growth and metal bioaccumulation: a potential Cd-hyperaccumulator and As-excluder Bidens pilosa L. J Hazard Mater 166(1–3):1023–1028. CrossRefGoogle Scholar
  42. Tananonchai A, Sampanpanish P (2014) Effect of EDTA and DTPA on cadmium removal from contaminated soil with water hyacinth. Appl Environ Res 36(3):65–76. CrossRefGoogle Scholar
  43. Tananonchai A, Sampanpanish P (2018) Phytotolerance, phytotoxicity and phytoremediation of Cd and EDTA mixtures with Napier grass. EnvironmentAsia 11(1):157–167. CrossRefGoogle Scholar
  44. Tian S, Lu L, Labavitch J, Yang X, He Z, Hu H, Sarangi R, Newville M, Commisso J, Brown P (2011) Cellular sequestration of cadmium in the hyperaccumulator plant species Sedum alfredii. Plant Physiol 157(4):1914–1925. CrossRefGoogle Scholar
  45. Trebolazabala J, Maguregui M, Morillas H, Diego AD, Madariaga JM (2017) Evaluation of metals distribution in Solanum lycopersicum plants located in a coastal environment using micro-energy dispersive x-ray fluorescence imaging. Microchem J 131:137–144. CrossRefGoogle Scholar
  46. Uera RB, Paz-Alberto AM, Sigua GC (2007) Phytoremediation potentials of selected tropical plants for ethidium bromide. Environ Sci Pollut Res 14(7):505–509. CrossRefGoogle Scholar
  47. USEPA (1996) Microwave assisted acid digestion of siliceous and organically based matrices (method 3052). Washington D.C., United States of AmericaGoogle Scholar
  48. USEPA (2000) Introduction to phytoremediation. National Risk Management Research Laboratory. Office of Research and Development, United States of America, Ohio, USAGoogle Scholar
  49. Wang YX, Specht WJ (2011) Stable isotope labeling and zinc distribution in grains studied by LA-ICP-MS in an ear culture system reveals zinc transport barriers during grain filling in wheat. New Phytol 189(2):428–437. CrossRefGoogle Scholar
  50. Wei SH, Zhou QX (2005) Phytoremediation of cadmium-contaminated soils by Rorippa globosa using two phase planting. Environ Sci Pollut Res 13(3):151–155. CrossRefGoogle Scholar
  51. Wiangkham N, Prapagdee B (2018) Potential of Napier grass with cadmium-resistant bacterial inoculation on cadmium phytoremediation and its possibility to use as biomass fuel. Chemosphere 201:511–518. CrossRefGoogle Scholar
  52. Wu LH, Luo YM, Xing XR, Christie P (2004) EDTA-enhanced phytoremediation of heavy metal contaminated soil with Indian mustard and associated potential leaching risk. Agriculture Ecosystem and Environment 102(3):307–318. CrossRefGoogle Scholar
  53. Xu WD, Lu GN, Dang Z, Liao CJ, Chen QP, Yi XY (2013) Uptake and distribution of cd in sweet maize grown on contaminated soils: a field-scale study. Bioinorg Chem Appl 2013:1–8. CrossRefGoogle Scholar
  54. Yang X, Baligar VC, Martens DC, Clark RB (1995) Influx, transport, and accumulation of cadmium in plant species grown at different Cd2+ activities. J Environ Sci Health 19(3–4):569–583. CrossRefGoogle Scholar
  55. Zaier H, Ghnaya T, Ghabriche R, Chmingui W, Lakhdar A, Lutts S, Abdelly C (2014) EDTA-enhanced phytoremediation of lead-contaminated soil by the halophyte Sesuvium portulacastrum. Environ Sci Pollut Res 21(12):7607–7615. CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Interdisciplinary Program in Environmental Science, Graduate SchoolChulalongkorn UniversityBangkokThailand
  2. 2.Environmental Research InstituteChulalongkorn UniversityBangkokThailand
  3. 3.Research Program of Toxic Substance Management in the Mining IndustryCenter of Excellence on Hazardous Substance ManagementBangkokThailand
  4. 4.Research Unit of Green Mining ManagementChulalongkorn UniversityBangkokThailand
  5. 5.Synchrotron Light Research InstituteNakhon RatchasimaThailand

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