Environmental Science and Pollution Research

, Volume 26, Issue 10, pp 9916–9930 | Cite as

Using multi-integrated biomarker indexes approach to assess marine quality and health status of marine organism: a case study of Ruditapes philippinarum in Laizhou Bay, China

  • Rongwang Ji
  • Luqing PanEmail author
  • Ruiming Guo
  • Lei Zheng
  • Mengyu Zhang
Research Article


With the progress of technology and the deepening of understanding of biological monitoring, much more attention has been paid to the multiple evaluation of marine pollution monitoring. In view of this, our study aimed at establishing a multi-integrated biomarker indexes approach to evaluate marine condition systematically and comprehensively. In the current study, sampling was conducted in Laizhou Bay, China (S1, S2, and S3) in May, August, and October of 2015. And then, multi-integrated biomarker indexes approach was applied to assess marine PAHs pollution, select appropriate biomarkers, and evaluate marine environmental quality and health status of the clams of Ruditapes philippinarum. As the results showed, S2 was the most PAHs-polluted site while S1 was the least polluted site, and the levels of tPAHs in seawater and sediments ranged from 69.78 to 315.30 ng/L and 163.19 to 565.17 ng/g d.w., respectively. And all three sampling sites had different sources of PAHs. IBR represented DNA damage (F value), the expression of SOD, EROD activity, GST activity, and LPO could be served as biomarkers to monitor the PAHs pollution in Laizhou Bay. And MPI suggested the quality of all three sites: S1 was generally favorable, S2 was moderately polluted, and S3 was lightly polluted. BRI values showed that the order of health status of R. philippinarum was S1 > S3 > S2.


Polycyclic aromatic hydrocarbons Ruditapes philippinarum Laizhou Bay Biological monitoring Multi-integrated biomarker indexes approach 



The work was supported by the State Ocean Administration Special Public Project of China (201405010). The authors thank all staffs at the Key Laboratory of Environmental Physiology of Aquatic Animal for the help with sampling and conducting all the tests.

Supplementary material

11356_2018_4082_MOESM1_ESM.rar (5.7 mb)
ESM 1 (RAR 5818 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Rongwang Ji
    • 1
  • Luqing Pan
    • 1
    Email author
  • Ruiming Guo
    • 1
  • Lei Zheng
    • 1
  • Mengyu Zhang
    • 1
  1. 1.Key Laboratory of Mariculture, Ministry of EducationOcean University of ChinaQingdaoChina

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