Environmental Science and Pollution Research

, Volume 26, Issue 8, pp 7743–7751 | Cite as

Field experiment on the effects of sepiolite and biochar on the remediation of Cd- and Pb-polluted farmlands around a Pb–Zn mine in Yunnan Province, China

  • Fangdong Zhan
  • Wenzeng Zeng
  • Xingchao Yuan
  • Bo Li
  • Tianguo Li
  • Yanqun Zu
  • Ming JiangEmail author
  • Yuan LiEmail author
Research Article


The effects of sepiolite and biochar on the contents of available nutrients (N, P, and K); the chemical forms and available contents of Cd and Pb in soils; the biomass and growth of maize; and the contents of nutrients, Cd, and Pb in maize were studied in situ in Cd- and Pb-polluted farmlands around the Lanping Pb–Zn mine in Yunnan Province, China. Results demonstrated that sepiolite did not influence the contents of available nutrients in soils, although it significantly increased the pH value and decreased available Cd (CaCl2-extractable and exchangeable) contents and exchangeable and reducible Pb. Moreover, sepiolite increased the biomass in the aboveground part of maize, resulting in the reduction of Cd contents in maize plants and grains by 25.6–47.5%. Meanwhile, the biochar increased the contents of available nutrients in soils and decreased the contents of exchangeable Pb in soils and biomass in the aboveground part of maize plants and grains; decreased the Cd contents in maize stems and grains by 26.7% and 24.6%, respectively; and decreased the Pb content in roots by 16.2%. However, neither sepiolite nor biochar had considerable influence on the Pb content in maize grains. According to a correlation analysis, soil pH has extremely significant negative correlations with available Cd content in soils, which in turn have extremely significant positive correlation with the Cd content in maize plants and grains. These results revealed that sepiolite increases soil pH and decreases Cd bioavailability in farmland soils around the Pb–Zn mine. Furthermore, biochar increases the contents of available nutrients in farmland soils and the maize yield. Sepiolite and biochar both decrease the contents and transfer coefficients of Cd in maize plants and grains and are, thus, applicable to the immobilization remediation of Cd-polluted farmlands.


Passivation material Cadmium pollution Lead pollution Immobilization remediation Chemical forms Soil properties 


Funding information

This publication is supported by the National Natural Science Foundation of China (41461093, 31560163), the Key Research Development Project in Yunnan Province (2018BB017), the Reserve Talents Fund for Young and Middle-Aged Academic and Technological leaders in Yunnan Province (2018HB043), the Innovation Team for Farmland Non-pollution Production of Yunnan Province (2017HC015), and the Project on Soil Pollution Control and Remediation of Farmland in Lanping County, Yunnan Province (YNBY2016-002).


  1. Abad-Valle P, Álvarez-Ayuso E, Murciego A, Pellitero E (2016) Assessment of the use of sepiolite amendment to restore heavy metal polluted mine soil. Geoderma 280:57–66CrossRefGoogle Scholar
  2. Abid M, Danish S, Zafar-Ul-Hye M, Shaaban M, Iqbal MM, Rehim A, Qayyum MF, Naqqash MN (2017) Biochar increased photosynthetic and accessory pigments in tomato (Solanum lycopersicum L.) plants by reducing cadmium concentration under various irrigation waters. Environ Sci Pollut R 24:22111–22118CrossRefGoogle Scholar
  3. Ahmad M, Rajapaksha A, Lim J, Zhang M, Bolan N, Mohan D, Vithanage M, Lee S, Ok Y (2014) Biochar as a sorbent for contaminant management in soil and water: a review. Chemosphere 99:19–33CrossRefGoogle Scholar
  4. Ahmad M, Lee S, Lee S, Al-Wabel M, Tsang D, Ok Y (2017) Biochar-induced changes in soil properties affected immobilization/mobilization of metals/metalloids in contaminated soils. J Soils Sediments 17:717–730CrossRefGoogle Scholar
  5. Ahmad M, Usman A, Al-Faraj A, Ahmad M, Sallam A, Al-Wabel M (2018) Phosphorus-loaded biochar changes soil heavy metals availability and uptake potential of maize (Zea mays L.) plants. Chemosphere 194:327–339CrossRefGoogle Scholar
  6. Bao S (2000) Soil and agricultural chemistry analysis. China Agriculture Press, BeijingGoogle Scholar
  7. Bian R, Joseph S, Cui L, Pan G, Li L, Liu X, Zhang A, Rutlidge H, Wong S, Chia C, Marjo C, Gong B, Munroe P, Donne S (2014) A three-year experiment confirms continuous immobilization of cadmium and lead in contaminated paddy field with biochar amendment. J Hazard Mater 272:121–128CrossRefGoogle Scholar
  8. Bradl H (2004) Adsorption of heavy metal ions on soils and soils constituents. J Colloid Interface Sci 277:1–18CrossRefGoogle Scholar
  9. Chen H, Teng Y, Lu S, Wang Y, Wang J (2015) Contamination features and health risk of soil heavy metals in China. Sci Total Environ 512-513:143–153CrossRefGoogle Scholar
  10. Cheng J, Li Y, Gao W, Chen Y, Pan W, Lee X, Tang Y (2018) Effects of biochar on Cd and Pb mobility and microbial community composition in a calcareous soil planted with tobacco. Biol Fertil Soils 54:373–383CrossRefGoogle Scholar
  11. Cui X, Hao H, Zhang C, He Z, Yang X (2016a) Capacity and mechanisms of ammonium and cadmium sorption on different wetland-plant derived biochars. Sci Total Environ 539:566–575CrossRefGoogle Scholar
  12. Cui L, Pan G, Li L, Bian R, Liu X, Yan J, Quan G, Ding C, Chen T, Liu Y, Liu Y, Yin C, Wei C, Yang Y, Hussain Q (2016b) Continuous immobilization of cadmium and lead in biochar amended contaminated paddy soil: a five-year field experiment. Ecol Eng 93:1–8CrossRefGoogle Scholar
  13. Duan Q, Lee J, Liu Y, Chen H, Hu H (2016) Distribution of heavy metal pollution in surface soil samples in China: a graphical review. B Environ Contam Tox 97:303–309CrossRefGoogle Scholar
  14. Gwenzi W, Muzava M, Mapanda F, Tauro T (2016) Comparative short-term effects of sewage sludge and its biochar on soil properties, maize growth and uptake of nutrients on a tropical clay soil in Zimbabwe. J Integr Agric 15:1395–1406CrossRefGoogle Scholar
  15. Hu H, Jin Q, Kavan P (2014) A study of heavy metal pollution in China: current status, pollution-control policies and countermeasures. Sustainability 6:5820–5838CrossRefGoogle Scholar
  16. Huang L, Li Y, Zhao M, Chao Y, Qiu R, Yang Y, Wang S (2018) Potential of Cassia alata L. coupled with biochar for heavy metal stabilization in multi-metal mine tailings. Inter J Env Res Pub Heal 15:E494CrossRefGoogle Scholar
  17. Jones B, Galan E (1988) Sepiolite and palygorskite. Rev Mineral Geochem 19:631–674Google Scholar
  18. Kocaoba S (2009) Adsorption of Cd(II), Cr(III) and Mn(II) on natural sepiolite. Desalination 244:24–30CrossRefGoogle Scholar
  19. Krekeler M, Guggenheim S (2008) Defects in microstructure in palygorskite-sepiolite minerals: a transmission electron microscopy (TEM) study. Appl Clay Sci 39:98–105CrossRefGoogle Scholar
  20. Leach D, Song Y, Hou Z (2017) The world-class Jinding Zn–Pb deposit: ore formation in an evaporite dome, Lanping Basin, Yunnan, China. Mineral Deposita 52:281–296CrossRefGoogle Scholar
  21. Lehmann J (2007) Bio-energy in the black. Front Ecol Environ 5:381–387CrossRefGoogle Scholar
  22. Li J, Xu Y (2018) Effects of clay combined with moisture management on Cd immobilization and fertility index of polluted rice field. Ecotox Environ Safe 158:182–186CrossRefGoogle Scholar
  23. Li Z, Ma Z, van der Kuijp TJ, Yuan Z, Huang L (2014) A review of soil heavy metal pollution from mines in China: pollution and health risk assessment. Sci Total Environ 468:843–853CrossRefGoogle Scholar
  24. Liang X, Han J, Xu Y, Sun Y, Wang L, Tan X (2014) In situ field-scale remediation of cd polluted paddy soil using sepiolite and palygorskite. Geoderma 235-236:9–18CrossRefGoogle Scholar
  25. Liang X, Xu Y, Xu Y, Wang P, Wang L, Sun Y, Huang Q, HUnag R (2016) Two-year stability of immobilization effect of sepiolite on Cd contaminants in paddy soil. Environ Sci Pollut R 23:12922–12931CrossRefGoogle Scholar
  26. Liu L, Li W, Song W, Guo M (2018) Remediation techniques for heavy metal-contaminated soils: principles and applicability. Sci Total Environ 633:206–219CrossRefGoogle Scholar
  27. Loganathan P, Vigneswaran S, Kandasamy J, Naidu R (2012) Cadmium sorption and desorption in soils: a review. Crit Rev Environ Sci Technol 42:489–533CrossRefGoogle Scholar
  28. Lucchini P, Quilliam RS, Deluca TH, Vamerali T, Jones DL (2014) Does biochar application alter heavy metal dynamics in agricultural soil? Agric Ecosyst Environ 184:149–157CrossRefGoogle Scholar
  29. Mahar A, Wang P, Li R, Zhang Z (2015) Immobilization of lead and cadmium in contaminated soil using amendments: a review. Pedosphere 25:555–568CrossRefGoogle Scholar
  30. Nie C, Yang X, Niazi N, Xu X, Wen Y, Rinklebe J, Ok Y, Xu S, Wang H (2018) Impact of sugarcane bagasse-derived biochar on heavy metal availability and microbial activity: a field study. Chemosphere 200:274–282CrossRefGoogle Scholar
  31. O'Connor D, Peng T, Zhang J, Tsang D, Alessi D, Shen Z, Bolan N, Hou D (2018) Biochar application for the remediation of heavy metal polluted land: a review of in situ field trials. Sci Total Environ 619-620:815–826CrossRefGoogle Scholar
  32. Quevauviller P, Rauret G, Muntau H, Ure A, Rubio R, López-Sánchez J, Fiedler H, Griepink B (1994) Evaluation of a sequential extraction procedure for the determination of extractable trace metal contents in sediments. Fresenius J Anal Chem 349:808–814CrossRefGoogle Scholar
  33. Rizwan M, Ali S, Abbas T, Adrees M, Zia-Ur-Rehman M, Ibrahim M, Abbas F, Qayyum MF, Nawaz R (2018) Residual effects of biochar on growth, photosynthesis and cadmium uptake in rice (Oryza sativa L.) under Cd stress with different water conditions. J Environ Manag 206:676–683CrossRefGoogle Scholar
  34. Sui F, Zuo J, Li L, Pan G, Crowley d (2018) Biochar effects on uptake of cadmium and lead by wheat in relation to annual precipitation: a 3-year field study. Environ Sci Pollut R 25:3368–3377CrossRefGoogle Scholar
  35. Sun Y, Xu Y, Xu Y, Wang L, Liang X, Li Y (2016a) Reliability and stability of immobilization remediation of Cd polluted soils using sepiolite under pot and field trials. Environ Pollut 208:739–746CrossRefGoogle Scholar
  36. Sun Y, Zhao D, Xu Y, Wang L, Liang X, Shen Y (2016b) Effects of sepiolite on stabilization remediation of heavy metal-contaminated soil and its ecological evaluation. Front Env Sci Eng 10:85–92CrossRefGoogle Scholar
  37. Sun Y, Sun G, Xu Y, Liu W, Liang X, Wang L (2016c) Evaluation of the effectiveness of sepiolite, bentonite, and phosphate amendments on the stabilization remediation of cadmium-contaminated soils. J Environ Manag 166:204–210CrossRefGoogle Scholar
  38. Taylor M, Mould S, Kristensen L, Rouillon M (2014) Environmental arsenic, cadmium and lead dust emissions from metal mine operations: implications for environmental management, monitoring and human health. Environ Res 135:296–303CrossRefGoogle Scholar
  39. Uchimiya M, Bannon DI (2013) Solubility of lead and copper in biochar-amended small arms range soils: influence of soil organic carbon and pH. J Agric Food Chem 61:7679–7688CrossRefGoogle Scholar
  40. Wang M, Zhu Y, Cheng L, Andserson B, Zhao X, Wang D, Ding A (2018) Review on utilization of biochar for metal-contaminated soil and sediment remediation. J Environ Sci 63:156–173CrossRefGoogle Scholar
  41. Wen H, Zhang Y, Cloquet C, Zhu C, Fan H, Luo C (2015) Tracing sources of pollution in soils from the Jinding Pb-Zn mining district in China using cadmium and lead isotopes. Appl Geochem 52:147–154CrossRefGoogle Scholar
  42. Xu Y, Liang X, Sun G, Sun Y, Qin X, Wang L (2009) Surface chemical characteristics of sepiolite and their mechanisms of adsorption of Pb2+, Cd2+, and Cu2+. J Agro-Environ Sci 28:2057–2063Google Scholar
  43. Xu Y, Liang X, Xu Y, Qin X, Huang Q, Wang L, Sun Y (2017) Remediation of heavy metal-polluted agricultural soils using clay minerals: a review. Pedosphere 27:193–204CrossRefGoogle Scholar
  44. Yang M, Xiao XY, Miao XF, Guo ZH, Wang FY (2012) Effect of amendments on growth and metal uptake of giant reed (Arundo donax L.) grown on soil contaminated by arsenic, cadmium and lead. T Nonferr Metal SOC 22:1462–1469CrossRefGoogle Scholar
  45. Yi Q, Dou X, Huang Q, Zhao X (2012) Pollution characteristics of Pb, Zn, As, Cd in the Bijiang river. Procedia Environ Sci 13:43–52CrossRefGoogle Scholar
  46. Yin D, Wang X, Chen C, Peng B, Tan C, Li H (2016) Varying effect of biochar on Cd, Pb and As mobility in a multi-metal contaminated paddy soil. Chemosphere 152:196–206CrossRefGoogle Scholar
  47. Yin X, Xu Y, Huang R, Huang Q, Xie Z, Cai Y, Liang X (2017) Remediation mechanisms for Cd-contaminated soil using natural sepiolite at the field scale. Environ Sci Proc Impacts 19:1563–1570CrossRefGoogle Scholar
  48. Zhang X, Yang L, Li Y, Li H, Wang W, Ye B (2012) Impacts of lead/zinc mining and smelting on the environment and human health in China. Environ Monit Assess 184:2261–2273CrossRefGoogle Scholar
  49. Zhao F, Ma Y, Zhu Y, Tang Z, McGrath S (2014) Soil contamination in China: current status and mitigation strategies. Environ Sci Technol 49:750–759CrossRefGoogle Scholar
  50. Zhou H, Jiao Y, Shi Z, Ming Q, Wang L, He L (2008) Magnetic analysis and assessment on heavy metal contamination in the farmland soil along Bijiang River in Yunnan Province. J Agro-Environ Sci 27:1586–1591Google Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Fangdong Zhan
    • 1
    • 2
  • Wenzeng Zeng
    • 1
    • 2
  • Xingchao Yuan
    • 1
    • 2
  • Bo Li
    • 1
    • 2
  • Tianguo Li
    • 1
    • 2
  • Yanqun Zu
    • 1
    • 2
  • Ming Jiang
    • 1
    • 2
    Email author
  • Yuan Li
    • 1
    • 2
    Email author
  1. 1.College of Resources and EnvironmentYunnan Agricultural UniversityKunmingChina
  2. 2.Yunnan Engineering Laboratory for Agro-environment Pollution Control and Eco-remediationYunnan Agricultural UniversityKunmingChina

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