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Environmental Science and Pollution Research

, Volume 25, Issue 30, pp 29880–29888 | Cite as

Oviposition deterrent activity of basil plants and their essentials oils against Tuta absoluta (Lepidoptera: Gelechiidae)

  • Boni Barthélémy Yarou
  • Thomas Bawin
  • Antoine Boullis
  • Stéphanie Heukin
  • Georges Lognay
  • François Jean Verheggen
  • Frédéric Francis
Chemistry, Activity and Impact of Plant Biocontrol products

Abstract

The leafminer Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) is one of the most important pests of tomato, reducing crop yields by up to 100% in greenhouses and fields, in several countries globally. Because synthetic insecticides lead to resistance and have adverse effects on natural enemies and the health of producers, alternative control methods are needed. In this study, we assessed the oviposition-deterring effect of basil plants, Ocimum gratissimum L. and O. basilicum L. (Lamiaceae), using dual-choice behavioural assays performed in flight tunnels. We found that both plants significantly reduced T. absoluta oviposition behaviour on a tomato plant located nearby. To evaluate the potential effect of basil volatile organic compounds, we formulated essential oils of both plant species in paraffin oil, and observed a similar oviposition-deterring effect. Gas chromatography analyses detected 18 constituents in these essential oils which the major constituents included thymol (33.3%), p-cymene (20.4%), γ-terpinene (16.9%), myrcene (3.9%) in O. gratissimum and estragol (73.8%), linalool (8.6%), β-elemene (2.9%) and E-β-ocimene (2.6%) in O. basilicum. Twenty and 33 compounds were identified of the volatiles collected on O. gratissimum and O. basilicum plants, respectively. The main components include the following: p-cymene (33.5%), γ-terpinene (23.6%), α-terpinene (7.2%), α-thujene (6.7%) and E-α-bergamotene (38.9%) in O. gratissimum, and methyl eugenol (26.1%), E-β-ocimene (17.7%), and linalool (9.4%) in O. basilicum. Four compounds (α-pinene, β-pinene, Myrcene, Limonene) were common in essential oils and plants. Our results suggest the valuable potential of basil and associated essential oils as a component of integrated management strategies against the tomato leafminer.

Keywords

O. basilicum O. gratissimum Essential oil Behaviour Oviposition Tuta absoluta Integrated management 

Notes

Acknowledgements

This research was funded by Erasmus Mundus program. Tuta absoluta facilities were funded by the Service Public de Wallonie (SPW–DGO3. D31-1344). The authors thank Frédéric Dresen for technical support in tomato plant cultures and insect rearing; Danny Trisman and Franck Michels for technical support in GC-MS analyses.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Boni Barthélémy Yarou
    • 1
  • Thomas Bawin
    • 1
  • Antoine Boullis
    • 1
  • Stéphanie Heukin
    • 2
  • Georges Lognay
    • 2
  • François Jean Verheggen
    • 1
  • Frédéric Francis
    • 1
  1. 1.Functional and Evolutionary Entomology, Agro Biochem Department, Gembloux Agro-bio TechUniversity of Liege (ULg)GemblouxBelgium
  2. 2.Analytical Chemistry, Agro Biochem Department, Gembloux Agro-bio TechUniversity of Liege (ULg)GemblouxBelgium

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