Environmental Science and Pollution Research

, Volume 25, Issue 11, pp 10317–10327 | Cite as

Eco-friendly and cost-effective Ag nanocrystals fabricated using the leaf extract of Habenaria plantaginea: toxicity on six mosquito vectors and four non-target species

  • Chinnadurai Aarthi
  • Marimuthu Govindarajan
  • Pichaimuthu Rajaraman
  • Naiyf S. Alharbi
  • Shine Kadaikunnan
  • Jamal M. Khaled
  • Ramzi A. Mothana
  • Nasir A. Siddiqui
  • Giovanni Benelli
Plant-borne compounds and nanoparticles: challenges for medicine, parasitology and entomology

Abstract

Recently, the biofabrication of metal nanoparticles has gained wide interest owing to its inherent features such as swift, simplicity, eco-friendliness, and cheaper costs. Different green-reducing agents led to the production of nanoparticles with varying toxicity on insects. In the current study, silver nanoparticles (AgNPs) were successfully synthesized using Habenaria plantaginea leaf extract. Ag nanoparticles were studied by UV–Vis spectroscopy (UV-Vis), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), scanning electron microscopy (SEM) coupled with energy-dispersive spectroscopy (EDS), and transmission electron microscopy (TEM). H. plantaginea extract and AgNPs were tested for mosquito larvicidal activity on Anopheles stephensi, Aedes aegypti, Culex quinquefasciatus, An. subpictus, Ae. albopictus, and Cx. tritaeniorhynchus. LC50 values were 102.51, 111.99, 123.47, 123.96, 136.56, 149.42 μg/ml and 12.23, 13.38, 14.78, 14.37, 15.39, 16.89 μg/ml, respectively. Moreover, H. plantaginea aqueous extract and AgNPs were tested against the non-target species Anisops bouvieri, Diplonychus indicus, Poecilia reticulata, and Gambusia affinis obtaining LC50 values ranging from 831.82 to 36,212.67 μg/ml. Overall, this study showed the effectiveness of H. plantaginea-fabricated nanoparticles on a wide range of important mosquito vectors, highlighting their scarce toxicity on four natural enemies predating mosquito larvae and pupae.

Keywords

Biofabrication Biosafety Biopesticide AFM, SEM, TEM Zika virus 

Notes

Acknowledgments

The authors extend their sincere appreciations to the Deanship of Scientific Research at King Saud University for funding the work through the research group project no. (RGP-073). The authors would like to thank the Principal and Head of the Department of Zoology, Thiru. Vi. Ka Government Arts College and the Professor and Head, Department of Zoology, Annamalai University for the laboratory facilities provided.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  1. 1.Department of ZoologyThiru. Vi. Ka. Government Arts CollegeTiruvarurIndia
  2. 2.Unit of Vector Control, Phytochemistry and Nanotechnology, Department of ZoologyAnnamalai UniversityAnnamalainagarIndia
  3. 3.Department of Botany and Microbiology, College of ScienceKing Saud UniversityRiyadhSaudi Arabia
  4. 4.Department of Pharmacognosy, College of PharmacyKing Saud UniversityRiyadhSaudi Arabia
  5. 5.Department of Agriculture, Food and EnvironmentUniversity of PisaPisaItaly

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