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Environmental Science and Pollution Research

, Volume 25, Issue 23, pp 22437–22445 | Cite as

Application of protein typing in molecular epidemiological investigation of nosocomial infection outbreak of aminoglycoside-resistant Pseudomonas aeruginosa

  • Min Song
  • Min Tang
  • Yinghuan Ding
  • Zecai Wu
  • Chengyu Xiang
  • Kui Yang
  • Zhang Zhang
  • Baolin Li
  • Zhenghua Deng
  • Jinbo Liu
Interface Effect of Ultrafine Mineral Particles and Microorganisms
  • 69 Downloads

Abstract

Pseudomonas aeruginosan has emerged as an important pathogen elated to serious infections and nosocomial outbreaks worldwide. This study was conducted to understand the prevalence of aminoglycoside (AMG)-resistant P. aeruginosa in our hospital and to provide a scientific basis for control measures against nosocomial infections. Eighty-two strains of P. aeruginosa were isolated from clinical departments and divided into AMG-resistant strains and AMG-sensitive strains based on susceptibility test results. AMG-resistant strains were typed by drug resistance gene typing (DRGT) and protein typing. Five kinds of aminoglycoside-modifying enzyme (AME) genes were detected in the AMG-resistant group. AMG-resistant P. aeruginosa strains were classified into three types and six subtypes by DRGT. Four protein peaks, namely, 9900.02, 7600.04, 9101.25 and 10,372.87 Da, were significantly and differentially expressed between the two groups. AMG-resistant P. aeruginosa strains were also categorised into three types and six subtypes at the distance level of 10 by protein typing. AMG-resistant P. aeruginosa was cloned spread in our hospital; the timely implementation of nosocomial infection prevention and control strategies were needed in preventing outbreaks and epidemic of AMG-resistant P. aeruginosa. SELDI-TOF MS technology can be used for bacterial typing, which provides a new method of clinical epidemiological survey and nosocomial infection control.

Keywords

Pseudomonas aeruginosa Aminoglycoside-modifying enzymes Surface-enhanced laser desorption/ionization-time of flight mass spectrometry Resistance Molecular typing Nosocomial infection 

Abbreviations

AMG

Aminoglycoside

DRGT

Drug resistance gene typing

AME

Aminoglycoside-modifying enzyme

SELDI-TOF MS

Surface-enhanced laser desorption/ionization-time of flight mass spectrometry

MDR

Multidrug-resistant

APH

Aminoglycoside phosphoryltransferase

AAC

Aminoglycoside acetyltransferase

ANT

Aminoglycoside nucleotidyl transferase

AFLP

Amplified fragment length polymorphism

RFLP

Restriction fragment length polymorphism

REP-PCR

Repetitive extragenic palindromic polymerase chain reaction

PFGE

Pulsed-field gel electrophoresis

MLST

Multilocus sequence typing

CLSI

Clinical Laboratory Standards Institute

MICs

Minimal inhibitory concentrations

ACTH

Adrenocorticotropic hormone

MALDI

Matrix-assisted laser desorption and ionization

Notes

Funding Information

This research was funded by the National Natural Fund Project of China (No. 41472046), the Key Program of National Natural Science Project of China (No. 41130746), the Science and Technology Project of Sichuan Province, China (No. 2016JY0045), the Strategic Cooperation Project of the Luzhou People’s Government and Sichuan University, China (No. 2013CDLZ-S15) and the Fund Project of the Science and Technology Leader of Sichuan Province (No. 15031).

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  • Min Song
    • 1
  • Min Tang
    • 1
  • Yinghuan Ding
    • 1
  • Zecai Wu
    • 1
  • Chengyu Xiang
    • 1
  • Kui Yang
    • 1
  • Zhang Zhang
    • 1
  • Baolin Li
    • 1
  • Zhenghua Deng
    • 1
  • Jinbo Liu
    • 1
  1. 1.Department of Laboratory Medicinethe Affiliated Hospital of Southwest Medical UniversityLuzhouChina

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