Environmental Science and Pollution Research

, Volume 25, Issue 30, pp 29901–29909 | Cite as

Inhibition of Phytophthora species, agents of cocoa black pod disease, by secondary metabolites of Trichoderma species

  • Gilles-Alex Pakora
  • Joseph Mpika
  • Daouda Kone
  • Michel Ducamp
  • Ismael Kebe
  • Bastien Nay
  • Didier BuissonEmail author
Chemistry, Activity and Impact of Plant Biocontrol products


Cocoa production is affected by the black pod disease caused by several Phytophthora species that bring, about each year, an estimated loss of 44% of world production. Chemical control remains expensive and poses an enormous risk of poisoning for the users and the environment. Biocontrol by using antagonistic microorganisms has become an alternative to the integrated control strategy against this disease. Trichoderma viride T7, T. harzanium T40, and T. asperellum T54, which showed in vivo and in vitro antagonistic activity against P. palmivora, were cultured and mycelia extracted. Inhibition activity of crude extracts was determined, and then organic compounds were isolated and characterized. The in vitro effect of each compound on the conidia germination and mycelia growth of four P. palmivora, two P. megakaria, and one P. capsici was evaluated. T. viride that displayed best activities produced two active metabolites, viridin and gliovirin, against P. palmivora and P. megakaria strains. However, no activity against P. capsici was observed. Besides being active separately, these two compounds have a synergistic effect for both inhibitions, mycelia growth and conidia germination. These results provide the basis for the development of a low-impact pesticide based on a mixture of viridin and gliovirine.


Cacao Phytophthora Trichoderma virens Viridin Gliovirin Synergistic effect 



The authors wish to thank C. Bance for technical assistance, L. Dubost for mass spectra, A. Deville for NMR spectra, and J. Mpika for providing the Trichoderma strains used in this study. The Government of Ivory Coast is acknowledged for the PhD fellowship to G.-A. P.).

Supplementary material

11356_2017_283_MOESM1_ESM.pptx (939 kb)
ESM 1 (PPTX 938 kb).


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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  1. 1.Sorbonne Universités, Muséum National d’Histoire Naturelle, Centre National de la Recherche Scientifique (CNRS UMR 7245)ParisFrance
  2. 2.Département de Biosciences, Laboratoire Pharmacodynamie BiochimiqueUniversité Felix Houphouët-BoignyAbidjanCôte d’Ivoire
  3. 3.Station de Recherche de Bimbresso, CNRAAbidjanCôte d’Ivoire
  4. 4.Laboratoire de Physiologie VégétaleUniversité de Cocody AbidjanAbidjanCôte d’Ivoire
  5. 5.UMR BGPI-CIRAD, TA A 54/K, Campus International de BaillarguetMontpellier Cedex 5France
  6. 6.Laboratoire de Phytopathologie, CNRADivoCôte d’Ivoire

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