3D Printed Loading Device for Inducing Cellular Mechanotransduction via Matrix Deformation
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This manuscript details the design, fabrication, characterization, and application of a 3D printed loading device for the investigation of cellular mechanotransduction pathways activated by matrix deformation. The device, which works as a screw jack, applies out-of-plane substrate distention to a thin polymer membrane via platen displacement. Load induces a strain gradient on the top surface of the membrane where cells are cultured. A high performance poly-lactic acid 3D filament was used for printing, resulting in a compact, cost-effective device that is fully autoclavable and compatible with standard laboratory incubators. The device was customized to accommodate a loadable polydimethylsiloxane chip developed in our lab for culturing MLO-Y4 osteocytes; however, the design can be easily adapted to load any mechanosensitive cells grown on an elastomeric membrane. Using finite element analysis, we demonstrated that the device can generate a range of strains to induce a variety of responses by the osteocytes. Cell viability data demonstrated that these ranges had the ability to engender load-induced apoptotic differences.
KeywordsMechanotransduction Matrix deformation Osteocytes Finite element analysis 3D printing
The authors would like to thank Mr. Stephen Paterson for his help with 3D printing. This work was supported by the National Science Foundation under Grant Nos. (CBET 1060990 and EBMS 1700299). Also, this material is based upon work supported by the National Science Foundation Graduate Research Fellowship Program under Grant No. (2018250692). Any opinion, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation.
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