Biochemical changes in oxidative stress markers following endurance training and consumption of purslane seed in rats with hydrogen peroxide-induced toxicity
- 50 Downloads
The current study examined fluctuations in oxidative stress markers following endurance training (ET) and consumption of purslane seeds (Ps) in rats after receiving H2O2. Fifty-four adult male Wistar rats were assigned to nine experimental groups: (1) control (intoxicated-no treatment); (2) ET; (3) ET + Ps 50 mg/kg/day; (4) ET + Ps 200 mg/kg/day; (5) ET + Ps 400 mg/kg/day; (6) Ps 50 mg/kg/day; (7) Ps 200 mg/kg/day; (8) Ps 400 mg/kg/day; (9) control (non-intoxicated, intact). The first eight groups were given 100 mg/kg of H2O2 to induce oxidative stress. Groups 2–5 were given ET for a period of 8 weeks. Heart and lung tissues were then exposed to evaluate the oxidative stress markers. Catalase, glutathione peroxidase, malondialdehyde, and superoxide dismutase enzymes were measured using ELISA kits. A marked improvement in enzyme concentration was observed in both tissues. It was more pronounced in the groups receiving higher doses of Ps + ET. The findings provide evidence that purslane seed supplementation has antioxidant potential alongside endurance training and improved the ability to cope with oxidative stress.
KeywordsOxidative stress Purslane seed Endurance training Hydrogen peroxide
This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
Compliance with ethical standards
Conflict of interest
None of the authors have financial or other conflicts of interest.
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
For this type of study, formal consent is not required.
- 11.Georgakouli K, Manthou E, Fatouros IG, Georgoulias P, Deli CK, Koutedakis Y, Theodorakis Y, Jamurtas AZ (2017) Enhanced erythrocyte antioxidant status following an 8-week aerobic exercise training program in heavy drinkers. Alcohol, FayettevilleGoogle Scholar
- 21.Lipinski B (2011) Hydroxyl radical and its scavengers in health and disease. Oxid Med Cell Longev 809696:9Google Scholar
- 22.Arshadi S, Azarbayjani MA, Hajaghaalipor F, Yusof A, Peeri M, Bakhtiyari S, Stannard RS, Osman NA, Dehghan F (2015) Evaluation of Trigonella foenum-graecum extract in combination with swimming exercise compared to glibenclamide consumption on type 2 diabetic rodents. Food Nutr Res 59:29717CrossRefGoogle Scholar
- 25.Jouybari MF, Farzanegi P, Barari AR (2014) The effect of 8-week aerobic exercise with purslane supplementation consumption on peroxidant and antioxidants indicators in women with type 2 diabetes. J Shahid Sadoughi Univ Med Sci 22:928–939Google Scholar
- 26.Salehi A, Farzanegi P (2015) Effect of 8 weeks of resistance training with and without Portulacalo seeds on some of liver injury markers in women with diabetes type 2. Urmia Med J 25:968–978Google Scholar
- 27.Esmaillzadeh A, Zakizadeh E, Faghihimani E, Gohari M, Jazayeri S (2015) The effect of purslane seeds on glycemic status and lipid profiles of persons with type 2 diabetes: A randomized controlled cross-over clinical trial. J Res Med Sci 20:47–53Google Scholar
- 28.Ahmed OM, Hozayen WG, Sree HTA (2015) Effects of ethanolic purslane shoot and seed extracts on doxorubicin-induced hepatotoxicity in albino rats, vol 2. World Academy of Science, Engineering, IstanbulGoogle Scholar
- 29.El-Azime ASA, Hussein EM, Ashry OM (2014) Synergestic effect of aqueous purslane (Portulaca oleracea L.) extract and fish oil on radiation-induced damage in rats. Int J Radiat Biol 90:1184–1190Google Scholar
- 35.Pal S, Chaki B, Chattopadhyay S, Bandyopadhyay A (2017) High intensity exercise induced oxidative stress and skeletal muscle damage in post-pubertal boys and girls: a comparative study. J Strength Cond Res 32:1045–1052Google Scholar
- 41.Gül M, Atalay M, Hänninen O (2003) Endurance training and glutathione-dependent antioxidant defense mechanism in heart of the diabetic rats. J Sports Sci Med 2:52Google Scholar
- 45.Lu J, Borthwick F, Hassanali Z, Wang Y, Mangat R, Ruth M, Shi D, Jaeschke A, Russell JC, Field CJ, Proctor SD, Vine DF (2011) Chronic dietary n-3 PUFA intervention improves dyslipidaemia and subsequent cardiovascular complications in the JCR:LA-cp rat model of the metabolic syndrome. Br J Nutr 105:1572–1582CrossRefGoogle Scholar
- 48.Jimoh F, Oladiji A (2005) Preliminary studies on Piliostigma thonningii seeds: proximate analysis, mineral composition and phytochemical screening. Afr J Biotechnol 4(12):1439–1442Google Scholar
- 50.Miller T, Wing J, Huete A (1984) The agricultural potential of selected C4 plants in arid environments. J Arid Environ 7(3):275–286Google Scholar