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Blocking C/EBP β protects vascular endothelial cells from injury induced by intermittent hypoxia

  • Yu Feng
  • Qingchu Li
  • Yinxiang Wu
  • Nana Zhao
  • Lu Li
  • Li LiEmail author
  • Liming ZhaoEmail author
Article
  • 19 Downloads

Abstract

Background

Intermittent hypoxia (IH) can damage endothelial cells and lead to apoptosis in obstructive sleep apnea-hypopnea syndrome (OSAHS). Hypoxia induces apoptosis in endothelial cells via upregulation of endothelin-1 (ET-1) and hypoxia inducible factor-1 alpha (HIF-1α) plays a key role in the hypoxic stress response.

Purpose

We investigated an approach to diminish the negative effect of HIF-1α while maintaining its protective effect.

Methods

Human umbilical vein endothelial cells (HUVECs) were subjected to sustained hypoxia (SH) or IH for 24 h, and the responses of HIF-1α, CCAAT/enhancer binding protein beta (C/EBP β), and endothelin-1 (ET-1) were assessed by western blotting. A luciferase reporter system was employed to verify the potential binding site (transcription factor binding site, TFBS) for C/EBP β in the ET-1 promoter. The specificity of regulation of ET-1 by HIF-1α via C/EBP β was evaluated by a lentiviral system. The effects of silencing of C/EBP β on IH-induced apoptosis, vascular endothelial growth factor (VEGF) protein levels, proliferation, and in vitro tube formation were studied.

Results

We found that IH significantly increased HIF-1α, C/EBP β, and ET-1 in HUVECs. Knockdown of HIF-1α or C/EBP β inhibited the upregulation of ET-1 induced by IH. Blocking C/EBP β impaired IH-induced apoptosis but did not affect VEGF expression, proliferation, or in vitro tube formation. C/EBP β was shown to mediate increased ET-1 transcription by HIF-1α through the TFBS, 5′-GTTGCCTGTTG-3′, in ET-1 promoter.

Conclusion

Silencing of C/EBP β can suppress apoptosis but does not affect the protective role of HIF-1α in the hypoxic stress response.

Keywords

OSAHS Intermittent hypoxia HIF-1α ET-1 C/EBP β 

Abbreviations

SH

Sustained hypoxia

IH

Intermittent hypoxia

OSAHS

Obstructive sleep apnea-hypopnea syndrome

HIF-1α

Hypoxia-inducible factor 1-alpha

C/EBP β

CCAAT/enhancer binding protein beta

HUVECs

Human umbilical vein endothelial cells

ET-1

Endothelin-1

VEGF

Vascular endothelial growth factor

TFBS

Transcription factor binding site

FBS

Fetal bovine serum

MOI

Multiplicity of infection

TBST

Tris-buffered saline with Tween 20

PVDF

Polyvinylidene fluoride

SDS-PAGE

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

Notes

Funding

The National Natural Science Foundation of China provided financial support in the form of national natural science funding (No. 81372529), the Natural Science Fund Project of Shanghai (13ZR1414400); the Innovation Fund Project of Education Commission in Shanghai (14ZZ079); and the Special issue of the Military Medical of Second Military Medical University (2012JS21). The sponsor had no role in the design or conduct of this research.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Department of Respiratory Medicine, Shanghai East HospitalTongji University School of MedicineShanghaiChina
  2. 2.Department of RadiologySecondary Affiliated Hospital of the Second Military Medical UniversityShanghaiChina
  3. 3.Department of Respiratory MedicineThird Affiliated Hospital of Second Military Medical UniversityShanghaiChina
  4. 4.Department of Teaching SupportSecond Military Medical UniversityShanghaiChina

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